Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Analysis and Application of a Recombinant Monooxygenase Library as a Biocatalyst for the Baeyer- Villiger Reaction

  • Park, Ji-Yeoun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Dong-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Su-Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jin-Hee (Korea Research Institute of Bioscience and Biotechnology) ;
  • Bae, Ki-Hwan (College of Pharmacy, Chungnam National University) ;
  • Lee, Choong-Hwan (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2007.07.31
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Abstract

Because of their selectivity and catalytic efficiency, BVMOs are highly valuable biocatalysts for the chemoenzymatic synthesis of a broad range of useful compounds. In this study, we investigated the microbial Baeyer-Villiger oxidation and sulfoxidation of thioanisole and bicyclo[3.2.0]hept-2-en-6-one using whole Escherichia coli cells that recombined with each of the Baeyer-Villiger monooxygenases originated from Pseudomonas aeruginosa PAOl and two from Streptomyces coelicolor A3(2). The three BVMOs were identified in the microbial genome database by a recently described protein sequence motif; e.g., BVMO motif(FXGXXXHXXXW). The reaction products were identified as (R)-/(S)-sulfoxide and 2-oxabicyclo/3-oxabicyclo[3.3.0]oct-6-en-2-one by GC-MS analysis. Consequently, this study demonstrated that the three enzymes can indeed catalyze the Baeyer-Villiger reaction as a biocatalyst, and effective annotation tools can be efficiently exploited as a source of novel BVMOs.

Keywords

References

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