• 한국식품영양과학회지
• /
• 제30권1호
• /
• pp.127-132
• /
• 2001

오미자 에탄올 추출물의 항미생물 활성을 측정하기 위하여 세균, 효모, 곰팡이가 포함되는 12종류의 미생물에 대한 항미생물 활성을 측정하였다. 추출물은 세균의 생육을 억제하지만 효모와 곰팡이의 생육은 억제하지 못하였다. B. subtilis와 S. aureus에 대한 최소치사농도는 1.6~3.2 mg/mL이었으며, E. coli를 비롯한 gram음성 세균의 최소치사농도는 6.3~12.5 mg/mL이였다. B. subtilis와 S. aureus의 생육은 각 각 900 ppm과 300 ppm에서 억제되었으며 gram음성 세균의 생육은 2,000 ppm 첨가시 4시간 정도 생육이 억제되었다. 오미자 추출물의 항균활성은 열처리에 안정하였으며 부위에 따른 추출물의 항균활성은 오미자 과육의 추출물이 주로 항균력을 나타내는 것으로 나타났다.

• International Journal of Oral Biology
• /
• 제41권2호
• /
• pp.75-81
• /
• 2016

Human mouth environment is known to include a variety bacteria, including Streptococcus spp., Staphylococcus spp., Actinomyces spp., Lactobacillus spp., Candida spp., Enterobacteriaceae, et al. Human oral microorganisms can cause dental caries, gingivitis, periodontitis, respiratory tract infection, and cardiovascular disease. Thus, right denture cleaning is essential to oral and general human health. The aim of this study was to evaluate the bactericidal effect of a sodium dichloroisocyanurate-based effervescent tablet (Aos Denti Germ, Aos Company, Chungbuk, Korea) against oral microorganisms. A total of 5 species Streptococcus spp. (Streptococcus anginosus, Streptococcus mitis, Streptococcus mutans, Streptococcus oralis, and Streptococcus sobrinus), Actinomyces oris, Candida albicans, and Escherichia coli were used in this study. All strains were exposed to the distilled water prepared with effervescent tablet. After the exposure, the mixture of strains and effervescent tablet was inoculated onto blood agar or MacConkey agar plate and cultured at $36^{\circ}C$. All strains were killed immediately on exposure to effervescent tablet. The results suggested that effervescent tablet could be used as an effective denture cleanser for dental hygiene.

• International Journal of Oral Biology
• /
• 제33권4호
• /
• pp.213-216
• /
• 2008

Continentalic acid (CA, (-)-pimara-8(14), 15-diene-19-oic acid) was isolated from the roots of Aralia cordata (Araliaceae) using bioassay-guided fractionation of a crude chloroform extract. The antibacterial activity of CA against Enterococcus faecalis and Enterococcus gallinarium was estimated by determining minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs). CA exhibited potent activity against standard vancomycin-resistant enterococci (VRE) and vancomycin-susceptible enterococci (VSE), with MICs and MBCs values between 4 and $8{\mu}g/mL$ and 4 and $16{\mu}g/mL$, respectively. This compound exhibited potent activity against strains of VRE, which are highly resistant to clinically useful antibiotics. These findings suggest that continentalic acid may be useful in controlling enterococcal infection.

• Journal of Microbiology and Biotechnology
• /
• 제17권4호
• /
• pp.663-667
• /
• 2007

Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. The bacteriocin (6,400 AU/ml) was inoculated with L. monocytogenes V7 and incubated for 0.5h, 1h, 3h, and 6h. The bacteriocin was found to destroy most of the cell wall and released most of the inclusions in the cells after 6 h of incubation. These results suggest that the bactericidal effect of the bacteriocin was due to bacterial lysis.

• 한국식품과학회지
• /
• 제31권3호
• /
• pp.864-869
• /
• 1999

식용식물 섬바디에서 분리한 falcarindiol과 monoglyceride (MG)와의 상승효과를 실험하였다. Falcarindiol 30 ppm 및 10 ppm과 MG 25 ppm, 10 ppm을 각각 배지에 첨가하여 Lm 5균주에 대한 혼탁도를 비교한 결과 4개의 처리구에서 시험된 모든 균에 대하여 혼탁도가 나타나지 않았다. 이 결과로 falcarindiol과 monoglyceride의 혼합물의 살균효과가 기대되어, Tryptic soy broth에 혼탁도 비교시 사용한 동일 농도를 첨가한 후, Lm 2균주(ATCC 19111, ATCC 19114)를 배양시켜 24시간 간격으로 생균수를 확인한 결과 대조구는 초기 $10^5{\sim}10^6\;CFU/mL$에서 72시간 배양결과 2 log cycle 증가하는 반면, 처리구는 72시간 배양결과 colony를 발견할 수 없었다. 이 결과를 볼 살균효과가 인정되었다. 이상의 결과로 섬바디로부터 분리된 falcarindiol과 monogyleride와의 혼합물은 살균효과가 단독 사용시보다 상승하였고, 합성 항균제보다 우수한 천연 항균제로서의 가능성이 있다고 평가된다.

• 한국식품과학회지
• /
• 제29권6호
• /
• pp.1236-1240
• /
• 1997

상백피를 75% 에탄올로 추출하여 얻은 조 추출물을 다시 용매별로 순차 분획 후 Listeria monocytogenes 5균주에 대하여 증식저해 효과를 비교한 후 항균활성 물질을 확인하였다. 활성이 있는 획분을 1차 및 2차 column chromatography를 통하여 활성 물질을 순수 분리하여 활성 물질 획분(F-5')을 얻었고 이 획분을 tryptic soy broth에 100 및 300 ppm을 첨가하여 Listeria monocytogenes를 증식시킨 결과 배양시간에 따라 뚜렷한 균수의 감소가 관찰되어 높은 살균효과가 인정되었으며 처리된 균체의 세포적의 비정상적인 형태가 전자현미경을 통하여 관찰되었다.

• Journal of Microbiology and Biotechnology
• /
• 제17권5호
• /
• pp.774-783
• /
• 2007

In the present study, acidocin 1B, a bacteriocin produced by Lactobacillus acidophilus GP 1B, exhibited profound inhibitory activity against a variety of LAB and pathogens, including Gram-negative bacteria, and its mode of action was to destabilize the cell wall, thereby resulting in bactericidal lysis. Acidocin 1B was found to be heat stable, because it lost no activity when it was heated up to $95^{\circ}C$ for 60 min. It retained approximately 67% of the initial activity after storage for 30 days at $4^{\circ}C$, and 50% of its initial activity after 30 days at $25^{\circ}C$ and $37^{\circ}C$. The molecular mass of acidocin 1B was estimated to be 4,214.65 Da by mass spectrometry. Plasmid curing results indicated that a plasmid, designated as pLA1B, seemed to be responsible for both acidocin 1B production and host immunity, and that the pLA1B could be transformed into competent cells of L. acidophilus ATCC 43121 by electroporation. Our findings indicate that the acidocin 1B and its producer strain may have potential value as a biopreservative in food systems.

• Food Science and Biotechnology
• /
• 제17권6호
• /
• pp.1345-1348
• /
• 2008

Titanium dioxide ($TiO_2$) shows antibacterial effects when exposed to near ultra violet (UV) light. In this study, $TiO_2$ photocatalytic continuous reactor was designed and applied to food-borne pathogens such as Vibrio parahaemolyticus ATCC 17802, Salmonella choleraesuis ATCC 14028, and Listeria monocytogenes ATCC 15313. $TiO_2$ films were prepared by conventional sol-gel dip-coating method using titanium tetra iso-propoxide (TTIP). The antibacterial activity of photocatalytic reactor with various flow rates and UV-A illumination time showed effective bactericidal activity. As the UV-A illumination time increased, survival rates of those bacteria decreased. After 60 min of UV-A illumination, the survival rates of V. parahaemolyticus and S. choleraesuis were less than 0.1%. However, that of L. monocytogenes was about 5% at that time point. These results present an effective way to exclude pathogenic bacteria from aqueous foods.

• Food Science and Biotechnology
• /
• 제17권4호
• /
• pp.850-856
• /
• 2008

White, red, and extruded ginsengs were studied against 8 strains of food-borne pathogens and/or food spoilage microorganisms. The ginseng powders were extracted with different extractants and screened for antimicrobial activity using the disc diffusion and broth dilution techniques. The results showed that the yield of extraction was higher with increase of aqueous solution content and temperature. Preliminary screening revealed that the red ginseng extracts were most active, that has been found to be highly effective against all tested microbe except Listeria monocytogenes. Moreover, Bacillus subtilis has shown highly susceptible, which the diameters of inhibition zone values of 28 extracts were between 7 and 14 mm. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) recorded for the different crude ginseng extracts against microorganism using ranged from 6.25 to 100 mg/mL, indicated that the methanol extract of ginseng were more effective than ethanol and water extracts. The 60% methanol extract of red ginseng had the greatest effects against B. subtilis with MIC and MBC values at 6.25 mg/mL.

• Food Science and Biotechnology
• /
• 제17권4호
• /
• pp.842-845
• /
• 2008

This study was carried out to investigate the bactericidal efficacy of concentration (0.1, 0.2, and 0.4 ppm) and exposure time (10 and 30 min) of ozone on bacterial reduction rate of Salmonella typhimurium KCTC 2541 and Staphylococcus aureus ATCC 13515 in the distilled water (DW), and DW supplemented with 0.2% soluble starch (SS), and metal ion (MC) using argentums (Ag) and copper (Cu). The significant bactericidal differences of S. aureus were showed in the treatments of DW and SS, respectively, at the concentration of ozone above 0.1 ppm for 10 min, comparing the respective initial bacterial counts. The bacterial reduction of S. aureus was more sensitive than that of S. typhimurum at the same concentration of ozone. The bacterial reduction rate of SS treatment was slightly lower than that of DW treatment at the same concentration of ozone (p<0.05), however, the bacterial reduction rate of strains improved in the MC treatment compared to the DW treatment at the same concentration of ozone.