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Morphological Changes Induced in Listeria monocytogenes V7 by a Bacteriocin Produced by Pediococcus acidilactici  

Heo, Seok (Department of Applied Biology and Chemistry, Konkuk University)
Lee, Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University)
Lee, Chi-Ho (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Min, Sang-Gi (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Park, Jong-Seok (Harzard Substances Analysis Team, Gyeongin Regional KFDA)
Kim, Hee-Yun (Harzard Substances Analysis Team, Gyeongin Regional KFDA)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.4, 2007 , pp. 663-667 More about this Journal
Abstract
Pediococcus acidilactici produces bacteriocin, which kills Listeria monocytogenes. The bactericidal mode of action of the bacteriocin against L. monocytogenes V7 was investigated by transmission electron microscopy. The bacteriocin was purified partially from the cell-free extract using Micro-Cel and cation-exchange chromatography, and the specific activity was increased 1,791 fold. The bacteriocin (6,400 AU/ml) was inoculated with L. monocytogenes V7 and incubated for 0.5h, 1h, 3h, and 6h. The bacteriocin was found to destroy most of the cell wall and released most of the inclusions in the cells after 6 h of incubation. These results suggest that the bactericidal effect of the bacteriocin was due to bacterial lysis.
Keywords
Bacteriocin; Pediococcus acidilactici; Listeria monocytogenes; transmission electron microscopy;
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Times Cited By KSCI : 7  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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