• Title/Summary/Keyword: BP test

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The correlation of bone probing, radiographic and histometric measurements (골탐침과 방사선학적 및 조직학적 계측의 상관관계)

  • Hwang, Sung-Joon;Kim, Chang-Sung;Lee, Doug-Youn;Lee, Yong-Keun;Cho, Kyoo-Sung;Chai, Jung-Kiu;Kim, Chong-Kwan;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.33 no.4
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    • pp.615-624
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    • 2003
  • The most accurate method to assess bone level is the histometric measurement. However it causes discomfort in patients and damage to the regenerated tissues. in the present study, we used 4 type regenerative therapies, The present study evaluated the clinical reliability and accuracy of bone probing measurements and radiographic bone level in the assessment of bone level by comparing those results with histometric confirmed bone level. Twentyfour(24) intrabony defects(4${\times}$4mm 1-wall intrabony defects) were surgically created in the mandibular second and fourth premolars of 6 beagle dogs. The control group underwent a conventional flap operation. Experimental group I was treated with calcium phosphate glass only, and while experimental group 2 was treated with GTR and experimental group 3 was treated with calcium phosphate glass and GTR. The subjects were sacrificed 8 weeks after the operation and a bone probing measurements, radiographic measurement and histometric measurement was performed. The correlation between bone probing measurements(BP) and histometric measurement(HL), and radiographic measurement(RL) and histometric measurement(HL) were analyzed with Spearman's rank correlation analysis and the statistical significance with respect to the type of regenerative therapies was analyzed with the Kruskal Wallis test. The coefficient of correlation to HL was 0.73 for RL and 0.90 for BP. The type of regenerative therapies had no significant effect on the difference between HL and other measurements. The results of this study suggests that bone probing measurements most closely represents actual bone level. So bone probing measurements may be a good clinical method for assessing the hone level following any type of periodontal regenerative therapies.

Analysis of Genetic Relationship of Entomogenous Fungi in Korea by Morphological Characteristics and RAPD (한국에서 채집한 Entomogenous fungi의 형태와 RAPD에 의한 유연관계 분석)

  • Choi, In-Young;Choi, Joung-Sik;Yu, Young-Jin;Lee, Wang-Hyu
    • The Korean Journal of Mycology
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    • v.29 no.1
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    • pp.34-40
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    • 2001
  • RAPD test and the observation of morphological, cultural characteristics of fourteen selected entomogenous fungi were conducted to investigate the analysis of their internal relationships. Paecilomyces tenuipes showed snow flower form attached to numerous white conidiophores, produced globular and semi-egg types on the club types of phialides. Cordyceps militaris formed globosely conidiophores, dark yellow fruiting body on pupa. The phialide as on Acremonium-type in global conidiophores. Beauveria bassiana covered with conidia was not formed fruiting body and adhered conidia on conidiophore of zigzag type. The PDA and SDAY medium were confirmed as an optimum growth of them. P. tenuipes showed to velvet and plane types in several media whereas C. militaris was belong to centrally raised and floccose in the morphological type. In contrast, B. bassiana covered with conidia on velvet shape. The size of amplified products were analyzed by RAPD using URP primer and were from 100 bp to 2.0 kb with $10{\sim}14$ geuomic DNA. Total similarities of two groups were by dendrogram of UPGMA analysis. The homology of P. tenuipes groups was 94.8 to 100%. It also showed 70.1 to 96.6% in C. militaris group and B. bassiana was higher similarity than any other. The internal change of C. militaris, produced telemorph fruiting body, was higher seperated in species than P. tenuipes and B. bassiana in the RAPD.

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Genotypic Identification in Commercial Strains of Pleurotus ostreatus based on AFLP and VCGs (AFLP 분석 및 체세포 불화합성에 의한 느타리 유사품종의 확인)

  • Seo, Kyoung-In;Yoo, Young-Bok;Jang, Kab-Yeul;Shin, Pyung-Gyun;Oh, YounLee;Kim, Kwang-Ho;Kong, Won-Sik
    • The Korean Journal of Mycology
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    • v.41 no.1
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    • pp.14-20
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    • 2013
  • We already reported four groups which contains some similar strains based on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism (AFLP) and vegetative compatibility group (VCG). AFLP analysis showed no difference among these strains except ASI 2595 and 2183 in Weonhyeong group and ASI 2829 in Suhan group. They showed specific DNA bands only in the result of P + AG/M + AAG and P + GT/M + ATG primer combinations out of eight different combinations. The AFLP primers produced a total of 330 fragments between 80 and 1000 bp in length for 31 Pleurotus ostreatus strains. At a genetic similarity of 0.96, the UPGMA analysis separated the isolates into four distinct clusters. Each group was classified by similar strains. Confrontation test by vegetative compatibility groups (VCGs) also showed distinct line between strains from different groups, but no line between similar strains within the cluster. Our results indicate that most of similar strains was not distinctness. Thus, similar strains are considered to be very close on the genealogy of their parent or same strain with different name.

Cloning of Coat Protein Gene from Korean Isolate Potato Leafroll Virus (PLRV) and Introduction into Potato (Solanum tuberosum) (한국 분리주 감자 잎말림 바이러스 (PLRV) 외피 단백질 유전자의 클로닝 및 감자 내 도입)

  • Seo Hyo-Won;Yi Jung-Yoon;Park Young-Eun;Cho Ji-Hong;Hahm Young-Il;Cho Hyun-Mook
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.243-250
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    • 2005
  • The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV.

Stem Rot on Ligularia fischeri Caused by Sclerotium rolfsii in Korea (Sclerotium rolfsii에 의한 곰취 흰비단병)

  • Moon, Youn-Gi;Kim, Se-Won;Choi, Jun-Keun;Kwon, Soon-Bae;Shim, Hong-Sik;Ju, Ho-Jong;Choi, In-Young
    • Research in Plant Disease
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    • v.21 no.1
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    • pp.36-39
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    • 2015
  • In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was $4-10{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.

Nutrients and Salt Consumption of Hypertension Patients According to Treatment Status (고혈압환자에서 치료상태에 따른 영양소 및 나트륨 섭취 양상)

  • Yim Juneeun;Cho Miran;Yin Chanesik;Seo Byune Kwan;Koh Hweone Gyun;Choue Ryowon
    • Journal of Nutrition and Health
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    • v.38 no.9
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    • pp.706-716
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    • 2005
  • High blood pressure is an important determinant of the incidence of coronary heart disease, stroke, congestive heart failure, renal failure, and peripheral vascular disease. Recommendations for control of high blood pressure emphasize lifestyle modification, including weight control, reduced sodium intake, increased physical activity. Subjects who were normotensive (n=19, $47.2\pm9.0$ y, BP l16/81 mmHg) ,treatment hypertensive (n=33, $54.2\pm6.9$ y, BP 132/85 mmHg) and non-treatment hypertensive (n=14, $50.1\pm11.0$ y, 149/94 mmHg) recruited. Anthropometric assessment (height weight waist circumference, hip circumference, fat$\%$, fat mass, and lean body mass) and dietary assessments (using 3-days food records, daily nutrient intakes were inuysed by CAN PRO 2.0 were carried out. Blood and 24-hour urine were collected). Test of recognition for salt taste threshold were performed. In non-treatment hypertensive male subjects, weight, $\%$IBW, BMI, and waist circumference were significantly higher than those of normotensive and treatment hypertensive subjects (p<0.05) .Food habits were not significantly different among the three groups. Intakes of vitamin A, vitamin B,, and vitamin B, were significantly higher in normotensive group (p<0.05). Intakes of sodium and salt taste recognition threshold were the highest in normotensive group and the lowest in treatment hypertensive group (p<0.05). Blood levels of lipids and minerals were not significantly different among the three groups. Urinary calcium level of normotensive group were significantly higher than that of treatment hypertensive and non-treatment hypertensive groups (p<0.05). These results indicate that continuous management of hypertension by drug and non-drug treatment affects salt taste recognition threshold and reduced the consumption of sodium. However, dietary sodium intake exceed recommended sodium intake to prevent and treat hypertension. It is necessary to develop the lifestyle modification program that may have beneficial effects on hypertension treatment.

A study of Growth Plate regression analysis using Tanner-Whitehouse 3 in hand AP of pediatrics (소아의 디지털 Hand 영상에서 TW3를 이용한 성장판의 회귀분석)

  • Lee, DongSeong;Jo, GuangSub;Lim, HanSub;Jeong, SeonKyoung;Jang, HwaYoung;Kim, SuHyun;Kang, SeSik;Kim, ChangSoo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2015.05a
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    • pp.391-394
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    • 2015
  • They evaluate the bone age using the GP-BP (Greulich-Pyle and Bayley-Pinneau) and TW3 (Tanner-Whitehouse 3) in clinical. The skeletal maturity in Hand AP is evaluated by clinical experience of physicians and this is qualitative evaluation not same in every physicians. In order to devise and evaluate new methods not using TW3 method in this situation. The study was conducted with 70 (Male 35, Female 35) children who visited Yangsan P Hospital from March 2014 to March 2015. The study measured the length of growth plate and distal proximal phalanx and conducted regression analysis for statistical significance test of bone age length difference. The study found average and standard deviation corresponding to certain ranges each bone age. The more bone age increase, the more the length of growth plate and distal proximal phalanx decreased. The girls have less average rather than the boys because bone grows fast. The girls have first period age of 12 to 14, it appears length variation significantly. The study conducted regression analysis and this has statistical significance.

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Antimicrobial Susceptibility and Biochemical Characteristics of Streptococcus suis Isolated from Diseased Pigs in Gyeongbuk Province (경북지방 환돈에서 분리한 Streptococcus suis의 생화학적 성상 및 약제감수성)

  • Choi, Seong-Kyoon;Kim, Seong-Guk;Kim, Young-Hoan;Choi, Jeong-Hye;Jo, Min-Hee;Cho, Gil-Jae
    • Journal of Life Science
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    • v.20 no.10
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    • pp.1549-1555
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    • 2010
  • Streptococcus suis is a worldwide pathogen of a variety of porcine infection and has also been described as a pathogen for humans. We studied biochemical characteristics, antimicrobial susceptibility, and identification of polymerase chain reaction (PCR) of S. suis isolated from diseased pigs in Gyeongbuk province from 2004 to 2009. Sixty-one isolates were identified as S. suis by biochemical characteristics and PCR from 40 farms. The biochemical characteristics of S. suis isolates were production of VP-negative, hippurate, esculin, and arginine decarboxylase-positive, and fermentation of carbohydrate was variable lactose, trehalose, inulin, and raffinose, which was typeable 11 phenotype. In an antimicrobial susceptibility test, the majority of isolates were highly susceptible to amoxicillin/clavulanic acid, ampicillin, cephalothin, cefoperazone and florfenicol, while being highly resistant to streptomycin, kanamycin, amikacin, neomycin, erythromycin, clindamycin, and tetracycline. The isolates were divided into 11 phenotypes of biochemistry. By using PCR, the 16S-rRNA gene DNA fragment was detected at 304 bp from all of isolates. These results may provide the basic information needed to establish strategies for the prevention of S. suis infection in pigs.

Effects of changing position on cardiac output & on patient's discomforts after cardiac surgery (개심술을 받은 환자의 체위에 따른 심박출량 및 불편감에 관한 연구)

  • Yu Mi;Kwon Eun-Ok;Choi Yun-Kyoung;Kang Hyun-Ju;Oh Se-Eun
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.7 no.2
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    • pp.256-270
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    • 2000
  • Invasive hemodynamic monitoring has become a valuable assessment parameters in critical care nursing in patients undergoing open heart surgery patients. During cardiac surgery, the Swan Ganz catheter is placed in the pulmonary artery. Critical care nurses routinely obtain cardiac output, cardiac index, and pulmonary arterial pressure in these patients. Traditionally, patients are positioned flat and supine for cardiac output measurement. Numerous studies have dealt with the effects of changing position on the hemodynamic variables. However, there are a few studies dealing with patients who undergo cardiac surgery in Korea. Thus, the purpose of this study was to determine the effects of changing position on cardiac output, PAP, CVP, BP, HR and discomfort in patients after cardiac surgery. A sample of 21 adults who had CABG and/or valve replacement with Swan Ganz catheters in place was studied. The data were collected in the cardiac ICU of a university hospital in Seoul during the period from July 28, 1999 to August 30. 1999. In this study, the independent variable is patient position in the supine, 30 degree, and 45 degree angles. Dependent variables are C.O., C.I., CVP, PAP, MAP, HR and patients' perceived discomforts. Subject discomfort was measured subjectively by visual analogue scale. Other hemodynamic data where collected by the thermodilution method and by direct measurement. The data were analyzed by percentile, t-test, ANOVA, Linear regression analysis using SPSS-/WIN program. The results are as follows : 1) Changes in cardiac output were absent in different angle positions, 0, 30. 45 degrees(F=.070, P=.932). Changes in cardiac index were absent in different angle positions, 0. 30, 45 degrees(P>.05). 2) Changes in central venous pressure were absent in different angle positions, 0, 30, 45 degree(P>.05). 3) PAP had no change in different angle 0, 30, 45 degree positions; systolic PAP(P>.05), diastolic PAP(P>.05). 4) Changes in systolic blood pressure were absent in different angle positions, 0, 30, 45 degree(P>.05). 5) Changes in heart rates were absent in different angle positions, 0, 30, 45 degree(P>.05). 6) Patients' perceived discomfort was absent in different angle positions, 0, 30, 45 degree(p<.05). In conclusion, critical care nurses can measure C.O., C.I., PAP, BP, & CVP in cardiac surgery patients at 30 degree or 45 degree positions. This can improve the patients' comfort.

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The Effectiveness of the Dispersant Use during the "Deepwater Horizon" Incident -REVIEW of the Proceedings from 2011 International Oil Spill Conference- (미국 멕시코만 기름유출사고에서 본 유처리제 사용의 효용성 고찰)

  • Cho, Hyun-Jin;Ha, Chang-Woo
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.18 no.1
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    • pp.61-65
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    • 2012
  • Once oil has spilled, oil spill responders use a variety of countermeasures to reduce the adverse effects of spilled oil on the environment. Mechanical methods of containment and recovery are preferred as the first response when the use of other methods fail or are ineffective. In these cases, the application of oil dispersants shall be use only as a last resort. While effectiveness of dispersants in removing oil form the sea surface is proven, the use of dispersants is controlled in almost all countries due to the toxicity of their active agents and the dispersed oil on the marine environment. However, according to reports, after dispersant application, no significant toxicity to fish or shrimp was observed in the field-collected samples. Moreover, the results also indicate that dispersant-oil mixtures are generally no more toxic to the aquatic test species than oil alone. During the Deepwater Horizon Incident, dispersants were applied to floating oil and injected into the oil plume at depth. These decisions were carefully considered by state and federal agencies, as well as BP, to prevent as much oil as possible from reaching sensitive shoreline habitats. Net Environmental Benefit Analysis for dispersant use assumed that dispersants appear to prevent long-term contamination resulting absence of oil in the substrate and will benefit marine wildlife by decreasing the risk of significant contamination to feathers or fur. Further study to use dispersants with scientific baseline is needed for our maritime environment which consistently threaten huge oil spill incidents occurrence.