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http://dx.doi.org/10.5423/RPD.2015.21.1.036

Stem Rot on Ligularia fischeri Caused by Sclerotium rolfsii in Korea  

Moon, Youn-Gi (Division of Agricultural Environment Research, Gangwon-do Agricultural Research and Extension Services)
Kim, Se-Won (Speciality Crops Research Institute Wild Vegetable Research Branch, Gangwon-do ARES)
Choi, Jun-Keun (Division of Agricultural Environment Research, Gangwon-do Agricultural Research and Extension Services)
Kwon, Soon-Bae (Division of Agricultural Environment Research, Gangwon-do Agricultural Research and Extension Services)
Shim, Hong-Sik (Crop Protection Division, National Academy of Agricultural Science, Rural Development Administration)
Ju, Ho-Jong (Department of Agricultural Biology, Chonbuk National University)
Choi, In-Young (Division of Climate Change Response, Jeollabuk-do Agricultural Research and Extension Services)
Publication Information
Research in Plant Disease / v.21, no.1, 2015 , pp. 36-39 More about this Journal
Abstract
In June 2012 and 2013, a destructive stem rot symptoms of Ligularia fischeri occurred sporadically in Hoengseong-gun and Pyeongchang-gun Gangwon-do, Korea. The typical symptom included water-soaking on the main stem, rotting, wilting and blighting, which eventually leads to death of the plant. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were white to brown, spherical or irregular, 1-3 mm in size on potato dextrose agar (PDA), The optimum temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was $4-10{\mu}m$. The typical clamp connections were observed in the hyphae of the fungus grown on PDA. The resulting sequence of 695 bp was deposited in GenBank. A BLAST search revealed that sequences of the this isolates showed >99% identity with those of Sclerotium rolfsii. On the basis of the morphological characteristics and phylogenetic analyses of molecular markers ITS rDNA, the fungi were identified as S. rolfsii. A pathogenicity test was carried out to fulfill Koch's postulates. To our knowledge, this is the first report of S. rolfsii on Ligularia fischeri in Korea.
Keywords
Ligularia fischeri; Sclerotium rolfsii; Stem rot;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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