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http://dx.doi.org/10.5010/JPB.2005.32.4.243

Cloning of Coat Protein Gene from Korean Isolate Potato Leafroll Virus (PLRV) and Introduction into Potato (Solanum tuberosum)  

Seo Hyo-Won (National Institute of Highland Agriculture)
Yi Jung-Yoon (National Institute of Highland Agriculture)
Park Young-Eun (National Institute of Highland Agriculture)
Cho Ji-Hong (National Institute of Highland Agriculture)
Hahm Young-Il (National Institute of Highland Agriculture)
Cho Hyun-Mook (National Institute of Highland Agriculture)
Publication Information
Journal of Plant Biotechnology / v.32, no.4, 2005 , pp. 243-250 More about this Journal
Abstract
The coat protein gene (AF296280) of the Korean isolate Potato leafroll virus (PLRV) was cloned and the open reading frame (627 bp) was transformed into potato (Solanum tuberosum cv. Superior). Out of seventeen individual transgenic lines, five lines were identified to confer resistance to PLRV through the five generation's selection program in the greenhouse as well as isolated trial field. Successful introduction and genetic stability of coat protein gene in the genome of potato were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and northern blot hybridization. Some of the transgenic lines were highly resistant to PLRV but did not show any resistance to less homologous Potato virus Y (PVY). Our results suggest that the resistance to PLRV is due to homology dependent gene silencing by sense strand coat protein gene. In addition, the results of field test through five generations showed that there were no significant differences comparing to nontransgenic potatoes in the morphological aspect of shoot as well as tuber, Ho remarkable differences were also observed in the major agronomic characters and yields except for the resistance to PLRV.
Keywords
Coat protein; PLRV; Solanum tuberosum; transgenic potato;
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