• 전기학회논문지
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• 제57권12호
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• pp.2313-2321
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• 2008

In order to develop reliable on-site partial discharge(PD) pattern recognition algorithm, the fuzzy neural network based on fuzzy set(FNN) and the polynomial network pattern classifier based on fuzzy Inference(PNC) were investigated and designed. Using PD data measured from laboratory defect models, these algorithms were learned and tested. Considering on-site situation where it is not easy to obtain voltage phases in PRPDA(Phase Resolved Partial Discharge Analysis), the measured PD data were artificially changed with shifted voltage phases for the test of the proposed algorithms. As input vectors of the algorithms, PRPD data themselves were adopted instead of using statistical parameters such as skewness and kurtotis, to improve uncertainty of statistical parameters, even though the number of input vectors were considerably increased. Also, results of the proposed neuro-fuzzy algorithms were compared with that of conventional BP-NN(Back Propagation Neural Networks) algorithm using the same data. The FNN and PNC algorithms proposed in this study were appeared to have better performance than BP-NN algorithm.

• 대한화학회지
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• 제55권4호
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• pp.620-625
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• 2011

구리수은막 전극(CBMFE)으로 전위차 역적정함으로써 이소니아자이드(INH)를 정량하는 간단하고 빠른 방법이다. 순수한 형태와 투약형태에 대해서 1.0-10.0 mg 범위에서 정량 할 수 있도록 적정조건을 설정하였다. 방법의 정밀도와 정확도는 통계적인 방법으로 평가되었으며, 정제와 시럽속에 함유된 INH 정량법은 F-시험과 t-시험을 통하여 영국약전(BP) 방법과 비교하였다.

• Perspectives in Nursing Science
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• 제10권2호
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• pp.111-119
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• 2013

Purpose: The purpose of this study was to determine the effects of 6 months lifestyle coaching program for adults with hypertension. Methods: The study utilized the one-group pretest-posttest design. The subjects were recruited from a corporation group member of a private health maintenance organization. The data of 35 subjects who completed the program out of initial 114 subjects were analyzed for this study. The program included 6 times of monthly face-to-face visits and additional short messages by cellular phone. They were given education for self-management of hypertension and coaching for lifestyle modification. Dependent variables were measured at the beginning and at the end of 6months program and compared by paired t-test. Results: There were significant reductions in systolic and diastolic BP, BMI, total cholesterol, and fasting blood sugar. Among the variables in life style, significant changes were shown in alcohol consumption (frequency and quantity) and exercise (frequency and duration). Conclusion: This study showed that 6 months lifestyle coaching program has potential for positive outcomes on BP control and modifying lifestyle for adults with hypertension. However, additional study is needed to find sustainability of lifestyle and the long-term effect of the program with larger sample.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.335-341
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• 2007

For the development of qualitative and quantitative PCR methods of genetically modified (GM) pepper developed in Korea, a capsanthin-capsorubin synthase (CCS) gene was used as the endogenous reference gene. The primer pair ccs-F/R amplifying the pepper endogenous gene gave rise to an amplicon of 102 bp. No amplified product was observed when DNA samples from 16 different plants were used as templates. The construct-specific primer pairs amplifying the junction region of the bar gene and Ti7 introduced in GM pepper gave rise to an amplicon of 182 bp. Quantitative PCR assay was performed using a TaqMan probe and a standard plasmid as a reference molecule, which contained both an endogenous and event-specific sequence. For the validation of this method, the test samples containing 0.1, 1, 3, 5, and 10% GM pepper were quantified.

• 식물병연구
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• 제15권2호
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• pp.88-93
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• 2009

고추 유묘의 신초가 말라죽고 잎에 수침상점무의 증상으로부터 병원균을 분리하여 균총의 색깔과 형태, 포자의 모양과 크기를 관찰한 결과 균총의 색깔은 처음에는 핑크색 띄나 차츰 회색으로 변하였고, 분생포자는 방추형으로 크기가 $8.1-17.0{\times}2.0-3.8{\mu}m$이며 생장최적온도 $25-27^{\circ}C$이다. 종 특이적 프라이머를 이용하여 고추 유묘에서 분리한 균을 동정한 결과 C. acutatum에 특이적인 프라이머인 CaINT에서만 496 bp의 증폭산물을 얻었다. 이들 결과를 바탕으로 유묘에서 분리한 균은 Collectotrichum acutatum으로 동정하였다. 고추 유묘에서 분리한 균주를 고추묘 생육단계별로 병원성 검정한 결과 유묘 뿐 아니라 식물체 전 생육기에 잎 탄저병 증상을 나타내었으며 또한 고추 열매에서도 강한 병원성을 나타내었다.

• 전기전자학회논문지
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• 제26권3호
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• pp.396-400
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• 2022

본 연구에서는 해저에서 발견한 인체를 해수면까지 운반하는 인양백을 기반으로 수중구조를 위한 ICT 기반 수중통신 모니터링 장치를 구현한다. 인양백은 에어를 주입하는 리프트백, 사이드백, CO₂ 카트리지 등으로 패키지화 되어 있다. 모니터링 시스템은 휴대하기 용이한 구조로 모바일 방식으로 개발한다. 수중 초음파 센서 신호는 USB 포트를 사용하여 공급되며, O/S는 리눅스로 구성한다. 수중통신동시험은 동해안에서 수심 6m~40m까지 실시간으로 측정 시험을 진행한다. 초음파 음향센서는 2,400bps로 변환되어 2원화에 따라 전송오차를 검증한다. 센서에서 모니터링까지 통신속도는 115,200bps, 제어기에서 수신부까지 통신속도는 2,400bps를 사용한다. 리프팅 장치의 상용화 단계에서 로우엔드 타입 개발이 용이하고 호환성이 넓도록 한다.

• 대한임상검사과학회지
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• 제47권2호
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• pp.83-89
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• 2015

결핵(Mycobacterium tuberculosis, MTB) 감염은 아직까지 전 세계에서 높은 유병률과 사망의 주요 원인이 되고 있으며 비정형 결핵(nontuberculous mycobacteria, NTM)은 최근 후천성 면역결핍증(AIDS)이나, 종양, 이식 등으로 면역력이 저하된 환자들의 임상 검체에서 분리빈도가 증가함에 따라 분리 균주의 임상적 의의가 중요시 되고 있다. 이에 항산균 염색을 이용한 객담 도말검사는 결핵균과 비정형 결핵균을 구별할 수 없다는 제한점이 있고, 균 분리배양검사는 시간이 오래 걸린다는 단점이 있어, 본 연구에서는 이러한 제한점을 가진 기존의 검사법을 대신하여 분자생물학적 방법인 중합효소 연쇄반응(polymerase chain reaction, PCR)을 이용하여 균 분리배양 검사와 비교하여 보았다. Mycobacteria를 동정하는데 항산균 염색과 3% ogawa 배지를 이용하였고, 균 분리배양 후 M. tuberculosis를 확인하기 위해서 niacin test를 실시한 결과 집락의 DNA를 추출하여 PCR후 동정된 M. tuberculosis와 niacin 양성이 일치함을 확인할 수 있었다. 또, 이 실험에서 항산성균 염색이 2+ 이상인 객담검체와 집락검체 각각에서 DNA를 추출하여 결핵균을 동정하는 방법으로 M. tuberculosis complex에 특징적으로 존재하는 insertion sequence (IS) 6110의 특정부위인 547 bp와 285 bp 부분을 증폭한 two-tube nested polymerase chain reaction을 시행하였고, 비정형 결핵균 동정법으로는 mycobacteria에 공통적으로 존재하는 rpoB 유전자 중 일부인 360 bp 부분을 증폭한 후, 제한효소 Msp I을 첨가 PCR-restriction fragment length polymorphism (RFLP)을 시행하였으며, 결핵균과 비정형 결핵균 모두 동정율에 거의 차이가 없었다. 1+인 경우는 객담검체에서 PCR한 결과가 31.2%에서 집락검체의 PCR한 결과 93.7%까지 결핵균과 비정형결핵균의 동정율이 높아졌고, trace인 경우 객담검체에서 PCR 결과가 2%에서 집락검체에서 PCR한 결과가 97.9%까지 결핵균과 비정형 결핵균의 동정율을 높일 수 있었다. 이 실험에서 항산성균 염색 1+이하 일때 객담검체와 집락검체로 PCR을 실시하면 결핵균과 비정형 결핵균의 동정율에 차이가 있고, 배양만으로는 결핵균의 동정은 가능하지만 비정형 결핵균의 동정이 가능하지 않으므로 배양검사와 PCR 검사 모두를 병행하므로써 보다 신속하고 정확한 검사결과를 내는데 도움 될 것이다.

• 생명과학회지
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• 제15권3호
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• pp.387-396
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• 2005

The aims of the study were to establish a short-term screening test for detecting testicular toxicity of chemicals in rats and to determine whether a 2-week administration period is sufficient to detect testicular toxicity of 2-bromopropane (2-BP) as an example. Male Sprague-Dawley rats were subcutaneously administered with 1000 mg/kg/day of 2-BP or its vehicle for 2 weeks. Ten male rats each were sacrificed on days 3, 7 and 14 after the initiation of treatment. Parameters of testicular toxicity included genital organ weights, testicular sperm head counts, epididymal sperm counts, motility and morphology, and qualitative and quantitative histopathologic examinations. The early histopathological changes observed on day 3 of treatment included degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, and decreased number of spermatogonia in stages II and V. On day 7 of treatment, atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, multinuclear giant cells, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in stages VII and XII. On day 14 after treatment, a significant decrease in the weights of testes and seminal vesicles was found. Atrophy of seminiferous tubules, exfoliation of germ cells, degeneration of spermatogonia and spermatocytes, mature spermatid retention, vacuolization of Sertoli cells, decreased number of spermatogonia in stages II and V, and decreased number of spermatocytes in all spermatogenic stages were also observed. In addition, a slight non-significant decrease in testicular sperm head counts, daily sperm production rate and epididymal sperm counts was found. The results showed that 2 weeks of treatment is sufficient to detect the adverse effects of 2-BP on male reproductive organs. It is considered that the short-term testicular toxicity study established in this study can be a useful tool for screening the testicular toxic potential of new drug candidates in rats.

• Journal of Microbiology
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• 제45권2호
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• pp.158-167
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• 2007

In this study, we have cloned a novel cDNA encoding for a papain-family cysteine protease from the Uni-ZAP XR cDNA library of the polychaete, Periserrula leucophryna. This gene was expressed in Escherichia coli using the T7 promoter system, and the protease was characterized after partial purification. First, the partial DNA fragment (498 bp) was amplified from the total RNA via RT-PCR using degenerated primers derived from the conserved region of cysteine protease. The full-length cDNA of cysteine protease (PLCP) was prepared via the screening of the Uni-ZAP XR cDNA library using the $^{32}P-labeled$ partial DNA fragment. As a result, the PLCP gene was determined to consist of a 2591 bp nucleotide sequence (CDS: 173-1024 bp) which encodes for a 283-amino acid polypeptide, which is itself composed of an 59-residue signal sequence, a 6-residue propeptide, a 218-residue mature protein, and a long 3'-noncoding region encompassing 1564 bp. The predicted molecular weights of the preproprotein and the mature protein were calculated as 31.8 kDa and 25 kDa, respectively. The results of sequence analysis and alignment revealed a significant degree of sequence similarity with other eukaryotic cysteine proteases, including the conserved catalytic triad of the $Cys^{90},\;His^{226},\;and\;Asn^{250}$ residues which characterize the C1 family of papain-like cysteine protease. The nucleotide and amino acid sequences of the novel gene were deposited into the GenBank database under the accession numbers, AY390282 and AAR27011, respectively. The results of Northern blot analysis revealed the 2.5 kb size of the transcript and ubiquitous expression throughout the entirety of the body, head, gut, and skin, which suggested that the PLCP may be grouped within the cathepsin F-like proteases. The region encoding for the mature form of the protease was then subcloned into the pT7-7 expression vector following PCR amplification using the designed primers, including the initiation and termination codons. The recombinant cysteine proteases were generated in a range of 6.3 % to 12.5 % of the total cell proteins in the E. coli BL21(DE3) strain for 8 transformants. The results of SDS-PAGE and Western blot analysis indicated that a cysteine protease of approximately 25 kDa (mature form) was generated. The optimal pH and temperature of the enzyme were determined to be approximately 9.5 and $35^{\circ}C$, respectively, thereby indicating that the cysteine protease is a member of the alkaline protease group. The evaluation of substrate specificity indicated that the purified protease was more active towards Arg-X or Lys-X and did not efficiently cleave the substrates with non-polar amino acids at the P1 site. The PLCP evidenced fibrinolytic activity on the plasminogen-free fibrin plate test.

• 생명과학회지
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• 제22권7호
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• pp.912-919
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• 2012

Carboxymethylcellulose (CM-cellulose)와 Beechwood xylan을 각각 기질로 사용하여 trypan blue를 첨가하여 제작한 Agar-LB 배지 상에서 명확한 활성환을 형성하는 균주를 cellulase와 xylanase 생산 균주로 단리하였다. 단리한 균주 유래의 16S rRNA 유전자 및 API 50 kit를 분석한 결과 Bacillus subtilis와 약 99.5%의 높은 상동성을 보였기에 본 균주를 Bacillus subtilis로 동정하여 B. subtilis NC1로 명명하였다. B. subtilis NC1 유래 cellulase와 xylanase는 CM-cellulose와 Beechwood xylan에 대하여 각각 높은 효소 활성을 보였으며, 두 효소 모두 pH 5.0과 $50^{\circ}C$의 조건하에서 가장 높은 효소 활성을 보였다. B. subtilis NC1 균주 유래 cellulase와 xylanase 유전자를 cloning하기 위하여 shot-gun cloning 방법을 이용하여 B. subtilis NC1 염색체 DNA로부터 효소 유전자를 cloning하여 유전자 배열을 규명한 결과 cellulase 유전자는 아미노산 499개를 암호화하는 1,500 bp의 open reading frame (ORF)으로 이루어져 있었으며, 아미노산 배열로부터 추정되는 분자량은 55,251 Da 이었다. 그리고, xylanase에 대한 유전자는 아미노산 422개를 암호화하는 1,269 bp의 ORF로 이루어져 있었으며 유전자 유래 아미노산 배열로부터 추정되는 단백질 분자량은 47,423 Da 이었다. 두 효소의 아미노산 배열을 이용하여 상동성을 검토한 결과 cellulase는 glycoside hydrolase family (GH) 5에 속하는 cellulase와 xylanase는 GH30에 속하는 xylanase와 높은 상동성을 나타내었다.