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http://dx.doi.org/10.5012/jkcs.2011.55.4.620

Potentiometric Back Titration of Isoniazid in Pharmaceutical Dosage Forms Using Copper Based Mercury Film Electrode  

Gajendiran, M. (Department of Inorganic Chemistry, Guindy Campus, University of Madras)
Nazer, M.M. Abdul Kamal (Post-Graduate and Research Department of Chemistry, The New College)
Publication Information
Journal of the Korean Chemical Society / v.55, no.4, 2011 , pp. 620-625 More about this Journal
Abstract
A simple, rapid potentiometric back titration of Isoniazid (INH) in the presence of Rifampicin (RIF) in tablets and syrups is described. The method is based on the oxidation of INH by a known excess of copper (II) ion and the back titration of unreacted copper (II) ion potentiometrically with ascorbic acid using a lab-made Copper Based Mercury Film Electrode (CBMFE). The titration conditions have been optimized for the determination of 1.0-10.0 mg of INH in pure and dosage forms. The precision and accuracy of the method have been assessed by the application of lack-of-fit test and other statistical methods. Interference was not caused by RIF and other excipients present in dosage forms. Application of the method for INH assay in tablets and syrups was validated by comparison of the results of proposed method with that of the British Pharmacopoeia (BP) method using F- and t- statistical tests of significance.
Keywords
Potentiometric back titration; Isoniazid; Pharmaceutical analysis; Copper based mercury film electrode; Statistical analysis;
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