Establishment of Quantitative Evaluation Method for Screening Testicular Toxicity in Rats: 2-Bromopropane as an Example
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Cha Shin-Woo
(Korea Institute of Toxicology, KRICT)
Bae Joo-Hyun (Korea Institute of Toxicology, KRICT) Son Woo-Chan (Huntingdon Life Sciences) Shin Jin-Young (College of Veterinary Medicine, Chonnam National University) Shin Dong-Ho (College of Veterinary Medicine, Chonnam National University) Kim Sung-Ho (College of Veterinary Medicine, Chonnam National University) Park Seung-Chun (College of Veterinary Medicine, Kyungpook National University) Kim Jong-Choon (College of Veterinary Medicine, Chonnam National University) |
1 | Chun, M. K. and J. C. Kim. 2000. Principles and methods for the reproductive-toxicological evaluation of new drug candidates. J. Toxicol. Pub. Health 16, 229-238 |
2 | Sharpe, R. M. 1994. Regulation of spermatogenesis. In: The Physiology of Reproduction. Second Edition. Knobil E and Neil JD (eds), pp. 1363-1434, Raven Press, New York |
3 | Son, H. Y, Y. B. Kim, B. H. Kang, S. W. Cho, C. S. Ha and J. K. Roh. 1999. Effects of 2-bromopropane on spermatogenesis in the Sprague-Dawley rat. Reprod. Toxicol. 13, 179-187 DOI ScienceOn |
4 | Son, W. C., J. C. Kim and I. J. Yu. 2003. The recommended approaches for the evaluation of testicular toxicity with awareness of the spermatogenic cycle and quantitative testicular toxicity evaluation methods. J. Toxicol. Pub. Health 19, 83-90 |
5 | Takayama, S., M. Akaike, K. Kawashima, M. Takahashi and Y. Kurokawa. 1995. A collaborative study in Japan on optimal treatment period and parameters for detection of male fertility disorders induced by drugs in rats. J. Am. Coll. Toxiciol. 14, 266-292 DOI |
6 | Takahashi, O. and S. Oishi. 2003. Testicular toxicity of dietarily or parenterally administered bisphenol A in rats and mice. Food Chem. Toxicol. 41, 1035-1044 DOI ScienceOn |
7 | Zenick, H. and E. Clegg. 1989. Assessment of male reproductive toxicity: a risk assessment approach. In Principles and Methods of Toxicology (Hayes AW, ed), Second Edition, pp. 275-309, Raven Press, New York |
8 |
Zhao, L. X., E. K. Kim, H. T. Lim, Y. S. Moon, N. H. Kim, T. H. Kim, H Choi, W. Chae, T. C. Jeong and E. S. Lee. 2002. Synthesis, characterization and in vitro identification of |
9 | Kim, J. C., D. H. Shin, S. H. Kim, J. K. Kim, S. C. Park, W. C. Son, H. S. Lee, J. E. Suh, C. Y. Kim, C. S. Ha and M. K. Chung. 2004b. Subacute toxicity study of a new camptothecin anticancer agent CKD-602 administered by intravenous injection to rats. Regul. Toxicol. Pharmacol. 40, 356-369 |
10 | Matsui, H. and M. Takahashi. 1999. A novel quantitative morphometry of germ cells for the histopathological evaluation of rat testicular toxicity. J. Toxicol. Sci. 24, 17-25 DOI ScienceOn |
11 | Omura, M., Y. Romero, M. Zhao and N. Inoue. 1999. Histopathological evidence that spermatogonia are the target cells of 2-bromopropane. Toxicol. Lett. 104, 19-26 DOI ScienceOn |
12 | Riecke, K. and R. Stahimann. 2000. Test systems to identify reproductive toxicants. Andrologia 32, 209-218 DOI ScienceOn |
13 | Robb, G. W., R. P. Amann and G. J. Killian. 1978. Daily sperm production and epididymal sperm reserves of pubertal and adult rats. J. Reprod. Fertil. 54, 103-107 DOI ScienceOn |
14 | Russel, J. D., R. A. EttIin, A. P. Sinha Hikim and E. D. Clegg. 1990. Histological and Histopathological Evaluation of The Testis. pp. 1-286, Cache River Press, Oearwater, Florida |
15 | Sakai, T., M. Takahashi, K. Mitsumori, K. Yasuhara, K. Kawashima, H. Mayahara and Y. Ohno. 2000. Collaborative work to evaluate toxicity on male reproductive organs by repeated dose studies in rats: overview of the studies. J. Toxicol. Sci. 25 (Suppl. 1), 1-21 |
16 | Seed, J., R. E. Chapin, E. D. Clegg, L. A. Dostal, R. H. Foote, et al. 1996. Methods for assessing sperm motility, morphology, and counts in the rat, rabbit, and dog: a consensus report. Reprod. Toxicol. 10, 237-244 DOI ScienceOn |
17 | Kim, J. C., K. H. Lim and M. K. Chung. 1999. Testicular cytotoxicity of DA-125, a new anthracycline anticancer agent, in rats. Reprod. Toxicol. 13, 391-397 DOI ScienceOn |
18 | Kim, J. C., S. H. Kim, D. H. Shin, T. H. Ahn, H. C. Kim, Y. B. Kim, C. Z. Jiang, J. Han and M. K. Chung. 2004a. Effects of prenatal exposure to the environmental pollutant 2-bromopropane on embryo-fetal development in rats. Toxicology 196, 77-86 DOI ScienceOn |
19 | Bailey, S. A., R. H. Zidell and R. W. Perry. 2004. Relationships between organ weight and body/brain weight in the rat: what is the best analytical endpoint? Toxicol. Pathol. 32, 448-466 DOI ScienceOn |
20 | Boekelheide, K., S. P. Darney, G. P. Daston, R. M. David, U. Luderer, A. F. Olshan, W. T. Sanderson, C. C. Willhite and S. Woskie. 2004. NTP-CERHR Expert Panel Report on the reproductive and developmental toxicity of 2-bromopropane. Reprod. Toxicol. 18, 189-217 DOI ScienceOn |
21 | Chung, M. K., S. J. Lee, Y. B. Kim, S. C. Park, D. H. Shin, S. H. Kim and J. C. Kim. 2005. Evaluation of spermatogenesis and fertility in F1 male rats after in utero and neonatal exposure to extremely low frequency electromagnetic fields. Asian J. Androl. 7, 189-194 DOI ScienceOn |
22 | Chun, Y. H., J. H. Han and J. J. Yu. 2001. Reproductive toxicity assessment on 2-bromopropane using spermatogenesis stage classification and Sertoli cell indices. J. Toxicol. Pub. Health. 17, 267-272 |
23 | Creasy, D. M. 1997. Evaluation of testicular toxicity in safety evaluation studies: the appropriate use of spermatogenic staging. Toxicol. Pathol. 25, 119-131 DOI ScienceOn |
24 | Dostal, J. A., C. K. Faber and J. Zandee. 1996. Sperm motion parameters in vas deferens and cauda epididymal rat sperm. Reprod. Toxicol. 10, 231-235 DOI ScienceOn |
25 | ICH Harmonized Tripartite Guideline. 1993. Detection of toxicity to reproduction for medicinal products |
26 | Ichihara, G., N. Asaeda, T. Kumazawa, T. Tagawa, M. Kamijima, X. Yu, H. Kondo, T. Nakajima, J. Kitoh, I. J. Yu, Y. H. Moon, N. Hisanaga and Y. Takeuchi. 1997. Testicular and haematopoietic toxicity of 2-bromopropane, a substitute for ozone layer-depleting chlorofluorocarbons. J. Occup. Health 39,57-63 DOI ScienceOn |
27 | Kim, J. C., H. S. Lee, H. I. Yun and M. K. Chung. 2000. Evaluation of the testicular toxicity caused by 2-bromopropane in rats. Korean J. Vet. Res. 40, 361-371 |
28 | Creasy, D. M. 2001. Pathogenesis of male reproductive toxicity. Toxicol. Pathol. 29, 64-76 DOI ScienceOn |
29 | Wu, X., A. S. Faqi, J. Yang, B. P. Pang, X. Ding, X. Jiang and I. Chahoud. 2002. 2-Bromopropane induces DNA damage, impairs functional antioxidant cellular defenses, and enhances the lipid peroxidation process in primary cultures of rat Leydig cells. Reprod. Toxicol. 16, 379-384 DOI ScienceOn |
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