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Qualitative and Quantitative Analysis of Genetically Modified Pepper  

Song, Hee-Sung (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University)
Kim, Jae-Hwan (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University)
Kim, Dong-Hern (Division of Biosafety, National Institute of Agricultural Biotechnology)
Kim, Hae-Yeong (Institute of Life Science & Resources and Graduate School of Biotechnology, Kyung Hee University)
Publication Information
Journal of Microbiology and Biotechnology / v.17, no.2, 2007 , pp. 335-341 More about this Journal
Abstract
For the development of qualitative and quantitative PCR methods of genetically modified (GM) pepper developed in Korea, a capsanthin-capsorubin synthase (CCS) gene was used as the endogenous reference gene. The primer pair ccs-F/R amplifying the pepper endogenous gene gave rise to an amplicon of 102 bp. No amplified product was observed when DNA samples from 16 different plants were used as templates. The construct-specific primer pairs amplifying the junction region of the bar gene and Ti7 introduced in GM pepper gave rise to an amplicon of 182 bp. Quantitative PCR assay was performed using a TaqMan probe and a standard plasmid as a reference molecule, which contained both an endogenous and event-specific sequence. For the validation of this method, the test samples containing 0.1, 1, 3, 5, and 10% GM pepper were quantified.
Keywords
Capsanthin-capsorubin synthase; GMO detection; GM pepper; real-time PCR;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
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