• Transactions on Electrical and Electronic Materials
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• 제9권5호
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• pp.213-219
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• 2008

Pulse waves continuously change with respect to the characteristics and status of the cardiovascular system and in relation to the blood pressure (BP) and the pulse wave velocity (PWV). Monitoring the vascular condition by analyzing the variations in pulse waveforms has been used to diagnose vascular disorders and in drug treatment of arteriosclerosis and peripheral circulatory obstruction. In this paper, we investigated the vascular characteristic index with regard to the BP and classified by pulse wave signals. The pressure pulse wave and photoplethysmography (PPG) were measured simultaneously while subjects exercised, producing changes in the BP, to analyze the variation in the vascular characteristic index. We investigated the correlation between the BP and vascular characteristic index with regard to the classification methods of the pulse wave. The reflection index (RI) and vascular stiffness index were correlated with the diastolic BP, but no correlation was found between these parameters and the systolic BP. These results suggest the possibility of estimating BP through simple measurements of pulse waves.

• 한국수산과학회지
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• 제55권4호
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• pp.455-460
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• 2022

Oxybenzone (Benzophenone-3; BP-3) is a compound used in sunscreens and enters the oceans due to improper disposal. It is known to disrupt the endocrine signaling in marine organisms, leading to immune and reproductive abnormalities in corals, shellfish and fishes. In this study, we investigated the effects of different concentrations of BP-3 (0, 10, 100 and 1,000 ㎍/L) on the fertilized eggs and hatched larvae of Oplegnathus fasciatus. The morphological changes and hatching and survival rates during embryo development were assessed. In addition, the levels of triiodothyronine (T3) were also measured. The hatched larvae exposed to BP-3 at concentrations of 10 and 100 ㎍/L for 24 h displayed malformation of the tail. After 36 h of exposure to BP-3, spinal deformity was observed at all tested concentrations. The hatching rate was significantly low when exposed to 100 ㎍/L of BP-3. A high levels of T3 was observed when the larvae were exposed to BP-3 at a concentration of 1,000 ㎍/L for 96 h (the end of the experiment). This may be related to increased size of larvae at 1,000 ㎍/L BP-3. In conclusion, our results suggested that BP-3 may interfere with embryo development, resulting in a reduction in hatching rate and malformation of larvae.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.55-55
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• 2022

Corylopsis Siebold & Zucc. (Hamamelidaceae) is widely used for horticultural plant and comprise ca. 25 species in East Asia (1 species in Korea; 4 species in Japan; 20 species in China). Previous revisions have gone from 7 to more than 30 species, causing confusion in the nursery industry and public gardens. Due to morphological similarity within Corylopsis, molecular research is needed to distinguish it. In this study, the chloroplast genome of C. gotoana and C. pauciflora distributed in Japan was completed by using NGS (Next-Generation Sequencing) technique. The genome size of C. gotoana and C. pauciflora were 159,434 bp (large single-copy (LSC): 88,164 bp; small single-copy (SSC): 18,702 bp; inverted repeat regions (IRs): 26,284 bp) and 159,363 bp (LSC: 88,097 bp; SSC: 18,700 bp; IRs: 26,283 bp), respectively. In addition, we investigated the repeats, SNPs, and indels, and that could be used as DNA markers. Phylogenetic analysis demonstrated that C. pauciflora was sister to C. gotoana and C. spicata. The genus Corylopsis is a monophyletic group and Loropetalum is closely related to Corylopsis. The results of our study will provide the basic data necessary for the analysis of the species identification markers and genetic diversity within the genus Corylopsis in the future.

• Advances in concrete construction
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• 제17권3호
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• pp.167-177
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• 2024

Disposal of waste glass derived from bottle or packaging glass, flat glass, domestic glass is one of the major environmental defies. Moreover, the remnants of bricks resulting from the remnants of buildings are also considered an important factor in polluting the environment due to the difficulty of filling or getting rid it. The aim of this study is to valorize these wastes through chemical activation to be an environmentally friendly material. The Microstructure, compressive strength, setting time, drying shrinkage, water absorption of different pastes produced by clear glass (CG), green glass (GG) and brick waste (BP) activated were tested and recorded after curing for 3, 7, 28 and 365 days. Five samples of pastes were mixed in proportions represented by: 100% GP (GP), 100% GGP (GGP), 100% BP (BP), 90% GP + 10% BP (GPB) and 90% GGP + 10% BP (GGPB). Various parameters considered in this study include sodium hydroxide concentrations (10 mol/l); 0.4 as alkaline liquid to binder ratio; 2.5 as sodium silicate to sodium hydroxide ratio and cured at 60℃ for 24 hours. Experimental results revealed that the addition of 10% of BP resulted in an increased strength performance of geopolymer paste especially with GGPB compared to GGP in 365 days. In addition, the 10% amount of BP increases the absorption and shrinkage rate of geopolymer pastes (GPB and GGPB) by reducing the setting time. SEM results revealed that the addition of BP and GP resulted in a dense structure.

• 생명과학회지
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• 제16권3호
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• pp.382-386
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• 2006

고양이의 혈통 등록을 위한 개체식별 및 친자판정을 목적으로 microsatellite DNA다형을 조사한 결과 다음과 같은 성적을 얻었다. 고양이 20두를 대상으로 microsatellite DNA다형의 대립유전자를 조사한 본 연구에서는 관찰된 대립유전자의 수는 $3{\sim}8$개 (평균 5.5개)이며 marker별 대립유전자는 FCA005 142bp (0.3750), FCA026 148 bp (0.5500), FCA075 136 bp (0.5000), FCA105 191 bp (0.4250), FCA224 156 bp (0.7750), FCA229 166 bp (0.6500), FCA240 163 bp (0.3000), FCA293 185 bp (0.5000), FCA453 186 bp (0.5500), FCA651 134 bp (0.6750) 대립유전자가 높은 빈도로 관찰되었다. Expected heterozygosity와 PIC는 각각 $0.390{\sim}0.827$(평균 0.639), $0.357{\sim}0.780$(평균 0.581)으로 나타났고 FCA240의 marker는 PIC value가 0.70 이상이었다. 또한 PE는 $0.076{\sim}0.444$으로서 10개 marker를 조합시 total PE는 0.9441로 관찰되었다. 10개의 microsatellite DNA다형 좌위를 가지고 친자관계를 분석한 결과 8두 중에서 1두(12.50%)가 모순으로 판정되었다. 또한 국내에서 사육중인 고양이의 개체식별 및 친자판정에 microsatellite marker를 활용할 수 있을 것으로 사료된다.

• 생물정신의학
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• 제1권1호
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• pp.67-78
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• 1994

The author examined the relationship of two markers, D5S39(p105-153Ra), D5S76(p105-599Ha) of chromosome 5 and $D_2$, $D_3$ receptor genes in a Korean schizophrenic pedigree using polymerase chain reaction(PCR). The results were as follows : 1) On D5S39 locus, 5 different alleles(224/226 bp : 4 cases, 218/226 bp : 3 cases, 222/226 bp : 3 cases, 218/230 bp : 1 case, 222/224 bp : 1 case) were produced. 2) On D5S76 locus, 5 different alleles(102/112 bp : 4 cases, 94/112 bp : 3 cases, 108/112 bp 3 cases, 94/94 bp : 1 case, 102/108 bp 1 case) were produced. 3) On $D_2$ receptor gene, 3 different alleles($A_1A_2$ : 8 cases, $A_1A_1$ : 2 cases, $A_2A_2$ : cases) were produced. 4) On $D_3$ receptor gene, 2 different alleles(1/2 : 7 cases, 1/1 : 5 cases) were produced. The author had not find any specific alleles on all four loci in all pedigree nor any specific alleles in the schizophrenic patients. Though the author has not found absolute relationship between the four loci and the onset of schizophrenia, there still remains the possibilities if the more detailed and elaborated pedigree studies are done.

• 한국자원식물학회지
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• 제24권5호
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• pp.584-590
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• 2011

의성종 마늘의 유묘로부터 상처(wound)에 특이적으로 발현되는 cytochrome P450 유전자군의 하나인 P450-Esg cDNA를 탐색하였다. P450-Esg는 1,419 bp의 open reading frame(ORF)을 가지고 473개의 아미노산을 가진 polypeptide를 코딩하는 것으로 나타났다. 국내와 몽골로부터 수집한 12개의 재배종으로 부터 P450-Esg 유사 유전자의 염기서열을 비교한 결과 시작코돈(ATG)에서 472~510 bp 및 1,210~1,240 bp 부위의 염기에서 재배종간에 차이를 보이는 염기서열을 다수 확인하였다. cDNA 1,210~1,240 bp의 부위는 P450 유전자에서 공통적으로 알려진 heme binding domain으로, 각 지역에서 수집된 재배종은 염기서열뿐만 아니라 아미노산 서열에 있어서도 특이적인 변이를 보였다. cDNA 472~510 bp 부위에서 코딩하는 13개 아미노산의 서열은 12개 재배종에서 모두 동일하였으나, 13개 아미노산 중 7개에서 재배종 마다 각각 다른 DNA 염기로 코딩하는 단일 염기다형성(single nucleotide polymorphism)을 보이는 서열을 확인하였다. 이 결과는 다양하게 존재하는 국내외 마늘 재배종을 구분하는 marker로 사용될 것이며, 외국산 마늘에 대한 유전적 우선권을 확보하는 수단으로 사용될 것이다.

• 미생물학회지
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• 제41권2호
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• pp.125-128
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• 2005

S. chungbukensis DJ77로부터 16S rRNA유전자의 염기서열을 분식하였다. 염기서열은 총 1,502 bp로 2000 년에 등록된 부분 서열(1,435 bp)보다 5' 방향과 3' 방향으로 29 bp와 37 bp 길이만큼 각각 확장하였으며, 1 bp가 추가로 삽입되었다. E. coli의 16S rRNA유전자를 모델로 이차구조를 제작하였으며, 네 부위가 특이적임을 발견하였다. Sphnigomonas spp.의 16S rRNA 서열과 S. chungbukensis DJ77의 다중서열검색 결과, Sphingomonas종에서만 나타나는 보존부위와 가변부위를 발견할 수 있었다. 특히, Campylobacter jejuni에서만 나타나는 것으로 알려진 긴 stem loop구조가 서열은 조금 다르지만 구조적 일치를 보이는 유사한 구조를 S. chungbukensis DJ77에서도 발견하였다. 결과적으로, 다중서열검색을 통해 제작한 계통수와 nucleotide signatures분석에 근거하여 S. chugukensis DJ77을 cluster II (Sphingobium)로 분류하였다.

• BMB Reports
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• 제48권7호
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• pp.407-412
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• 2015

The 12 kDa FK506-binding protein (FK506BP12), an immunosuppressor, modulates T cell activation via calcineurin inhibition. In this study, we investigated the ability of PEP-1-FK506BP12, consisting of FK506BP12 fused to the protein transduction domain PEP-1 peptide, to suppress catabolic responses in primary human chondrocytes and in a mouse carrageenan-induced paw arthritis model. Western blotting and immunofluorescence analysis showed that PEP-1-FK506BP12 efficiently penetrated chondrocytes and cartilage explants. In interleukin-1β (IL-1β)-treated chondrocytes, PEP-1-FK506BP12 significantly suppressed the expression of catabolic enzymes, including matrix metalloproteinases (MMPs)-1, -3, and -13 in addition to cyclooxygenase-2, at both the mRNA and protein levels, whereas FK506BP12 alone did not. In addition, PEP-1-FK506BP12 decreased IL-1β-induced phosphorylation of the mitogen-activated protein kinase (MAPK) complex (p38, JNK, and ERK) and the inhibitor kappa B alpha. In the mouse model of carrageenan-induced paw arthritis, PEP-1-FK506BP12 suppressed both carrageenan-induced MMP-13 production and paw inflammation. PEP-1-FK506BP12 may have therapeutic potential in the alleviation of OA progression. [BMB Reports 2015; 48(7): 407-412]

• Journal of Plant Biology
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• 제39권1호
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• pp.57-61
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• 1996

Thermal denaturation, reassociation kinectics 및 핵부피 측정 방법으로 고추 게놈의 염기 조성, kinetic component 및 게놈 크기를 조사하였다. 변성온도(Tm)에 근거한 고추 게놈의 염기 조성은 37% (G＋C)였으며, Cot 커브에 근거한 고추 게놈은 카피수가 10,754, 178 및 1이고 kinetic complexity가 $5.6{\times}10^{3}\;bp,\;1.9{\times}10^{6}\;bp\;및\;1.9{\times}10^{8}\;bp$인 3개의 성분이 게놈의 4.2%, 26% 및 65%를 차지하고 있었다. 이로부터 계산한 고추 게놈의 크기는 $1.25{\times}10^{9}\;bp/C$인데, 이는 핵부피($62.4\;\mu\textrm{m}^3/C$)로부터 계산한 $4.053{\times}10^{9}\;bp/C$의 약 33%에 해당한다.