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http://dx.doi.org/10.7732/kjpr.2011.24.5.584

Genetic Variation of Cytochrome P450 Genes in Garlic Cultivars  

Kwon, Soon-Tae (Department of Biological Resources, Andong Nat'l University)
Kamiya, Juli (Department of Horticulture, Purdue University)
Publication Information
Korean Journal of Plant Resources / v.24, no.5, 2011 , pp. 584-590 More about this Journal
Abstract
Wound inducible P450-Esg cDNA, one of cytochrome P450 gene family, was isolated from shoot of Euiseong garlic cultivar. P450-Esg cDNA possesses highly conserved heme-binding domain in the nucleotide sequence, and 1,419 bp of open reading frame (ORF) coding of 473 amino acids. Based on the nucleotide sequence analysis of P450-Esg homologous from twelve garlic cultivars, two domains, one domain between 472 to 510 bp, and the other between 1,210 to 1,249 bp from start codon (ATG), showed various nucleotide polymorphism among cultivars. Sequence of heme-binding domain in P450-Esg homologous, which is located at the domain between 1,210 to 1,240 bp from start codon, showed various nucleotide polymorphism as well as amino acid sequence polymorphism among twelve garlic cultivars. Anther domain, between 472 to 510 bp from start codon, showed exactly same amino acid sequence in the twelve garlic cultivars, but there were various single nucleotide polymorphism to the cultivars.
Keywords
cDNA; Cytochrome P450; Garlic cultivar; Polymorphism; Nucleotide sequence;
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1 Morita, A., T. Nakayama, N. Doba, S. Hinohara, T. Mizutani, M. Soma. 2007. Genotyping of triallelic SNPs using TaqMan PCR. Molecular and Cellular Probes 21:171-176.   DOI   ScienceOn
2 Nie, M. and W.H. Li. 1979. Methematical model for studying genetic variation in terms of restriction endonuclease. Proc. Natl. Acad. Soc. USA. 76:5269-5273.   DOI   ScienceOn
3 Ralston, L., S.T. Kwon, M. Schoenbeck, J. Ralston, D.J. Schnek, R.M. Coates and J. Chappell. 2001. Cloning, heterologous expression, and functional characterization of 5-epi-aristolochene-1,3-dihydroxylase from tobacco (Nicotiana tabacum). Arch. Biochem. Biophys. 393:222-235.   DOI   ScienceOn
4 Kwon S.T., J. Chappell. 1998. Elicitor-inducible 5-epiaristolochene hydroxylase in suspension cultures of tobacco (Nicotiana tabacum L.) Korean J. Plant Tiss. Cult. 25:141-146.
5 Kwon, S. T., I. J. Lee and J. Chappell. 1998. Cloning of elicitor-inducible 5-epi-aristolochene hydroxylase in tobacco cell suspension cultures. Korean J. Life Sci. 8:604-613.
6 Kwon, S.T. J.S. Kim, D.C. Jung and J.H. Jeong. 2003. Cloning of cytochrome P450 gene involved in the pathway of capsidiol biosynthesis in red pepper cells. Korean J. Life Sci. 13:879-888.   DOI
7 Kwon, S.T. and S.M. Oh. 1999. Genetic relationship among garlic cultivars based on RAPD analysis. Korean J. Life Sci. 9:671-676 (in Korean).
8 Lee, W.S. 1974. Studies on dormancy of Korean local garlics. J. Korean Soc. Hort. Sci. 15:257-264 (in Korean).
9 Harn, C., D. Choung and B. Kim. 1996. Studies on the karyotypes of Allium sativum. J. Korean Soc. Hort. Sci. 2:58-67.
10 Kollmann, F. and A. Shmida. 1977. Allium species of Mt. Hermon. I. Taxonomy. Israel J. Botany 26:128-148.
11 Hoshino, T., T. Yamaura, H. Imaishi, M. Chida, Y. Yoshizawa, K. Higashi, H. Ohkawa and J. Mizutani. 1995. 5-epi-Aristolochene 3-hydroxylase from green pepper. Phytochemistry 38:609-613.   DOI   ScienceOn
12 Hwang, J.M. 1993. Genetic divergence and classification of garlic cultivars by multivariate analysis. J. Korean Soc. Hort. Sci. 33:257-264 (in Korean).
13 Donaldson, R.P. and D.G. Luster. 1991. Multiple forms of plant cytochrome P450. Plant Physiol. 96:669-674.   DOI   ScienceOn
14 Cheshmedhiev, I. 1973. A cytotaxonomic investigation of the cultivated Allium species in Bulgaria. Genetikai Selektsiya 6:283-294.
15 Drabovich, A. P and S. Krylov. 2006. Identification of base pairs in single-nucleotide polymorphisms by MutS protein-mediated capillary electrophoresis. Anal. Chem. 78:2035-2038.   DOI   ScienceOn
16 Etoh, T. and H. Ogura. 1981. Peroxidase isozymes in the leaves of various clones of garlic, Allium sativum L. Mem. Fac. Agri. Kagoshima Univ. 17:71-77.
17 Bae, S.K., E.A. Jung and S.T. Kwon. 2010. Genetic variation and identification of RAPD markers from some garlic cultivars in Korea and Mongolia. Korean J. Plant Res. 23:458-464 (in Korean).