• 한국항해항만학회지
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• 제27권4호
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• pp.389-395
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• 2003

본 연구는 산업계 구조변화와 그에 따른 새로운 비즈니스 형태를 체계적으로 분류하고 해석하기 위한 이론과 개념을 수립하고 그것을 토대로 e-business를 포함한 새로운 비즈니스의 분류 프레임워크를 제안하는 것을 목적으로 한다. 제안한 모델은 비즈니스의 계층성에 주목한 비즈니스 계층(BL)의 개념, 업계를 연계하는 가치사슬(VC)의 개념, 그리고 실세계(혹은 실 주체)와 가상공간 세계(혹은 가상주체)의 개념을 상징 하는 R/V(Real/Virtual)의 개념을 기본 프레임으로 하여 구성되었으며, 이러한 BL-VC-R/V의 조합을 통해 이론상 해당업계에서 존재할 수 있는 모든 비즈니스의 분류가 가능하다는 점을 보였다. 한편, 본 연구에서는 제안된 프레임워크의 개념을 토대로 e-business의 새로운 정의를 제시하였고, 산업에 본 모델을 적용하여 공간시장화에 따른 구조변화와 그에 따른 비즈니스 분류를 행하였다. 그리고, 비즈니스차원의 관점에서 최근에 거론되고 있는 e-Logistics를 새롭게 정의하였다.

• Journal of Periodontal and Implant Science
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• 제52권2호
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• pp.141-154
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• 2022

Purpose: C57BL/6 mice, which are among the most common backgrounds for genetically engineered mice, are resistant to the induction of periodontitis by oral infection with periodontal pathogens. This study aimed to develop a periodontitis model in C57BL/6 mice using coaggregation between human pathogens and the mouse oral commensal Streptococcus danieliae (Sd). Methods: The abilities of Porphyromonas gingivalis ATCC 33277 (Pg33277), P. gingivalis ATCC 49417 (Pg49417), P. gingivalis KUMC-P4 (PgP4), Fusobacterium nucleatum subsp. nucleatum ATCC 25586 (Fnn), and F. nucleatum subsp. animalis KCOM 1280 (Fna) to coaggregate with Sd were tested by a sedimentation assay. The Sd-noncoaggregating Pg33277 and 2 Sd-coaggregating strains, PgP4 and Fna, were chosen for animal experiments. Eighty C57BL/6 mice received oral gavage with Sd once and subsequently received vehicle alone (sham), Fna, Pg33277, PgP4, or Fna+PgP4 6 times at 2-day intervals. Mice were evaluated at 5 or 8 weeks after the first gavage of human strains. Results: Fnn, Fna, and PgP4 efficiently coaggregated with Sd, but Pg33277 and Pg49417 did not. Alveolar bone loss was significantly higher in the PgP4 group at both time points (weeks 5 and 8) and in all experimental groups at week 8 compared with the sham group. The PgP4 group presented greater alveolar bone loss than the other experimental groups at both time points. A higher degree of alveolar bone loss accompanied higher bacterial loads in the oral cavity, the invasion of not only PgP4 but also Sd and Fna, and the serum antibody responses to these bacteria. Conclusions: Periodontitis was successfully induced in C57BL/6 mice by oral infection with a P. gingivalis strain that persists in the oral cavity through coaggregation with a mouse oral commensal bacterium. This new model will be useful for studying the role of human oral bacteria-host interactions in periodontitis using genetically engineered mice.

• 수산해양기술연구
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• 제31권1호
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• pp.74-83
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• 1995

모형 그물에 대한 어군행동의 수치모델링에 관한 연구의 일환으로서 어군행동의 모델링을 위하여 어군행동을 나타내는 운동방정식을 구성하는 각종 힘들의 parameter를 추정하기 위한 기초자료를 제공하고자, 모형 그물을 설치하지 않은 상태에서의 유속 변화에 따른 무지개송어의 유영행동을 화성처리하여 각 개체 위치좌표로부터 계산된 유영속도, 유영깊이, 수조의 변과 개체 사이의 거리, 개체상호간의 최근접거리, 어구의 3차원적 구조 등의 유역특성을 조사 분석한 결과는 다음과 같다. 유영궤적으로부터 무지개송어의 유영행동을 살펴보면, 정수상태에서는 수종 중앙부에서 유영하는 빈도가 높았으며, 수조 벽면으로부터 상당히 떨어져서 유회운동을 하는 것으로 나타났다. 정수상태에서의 평균 유영속도는 21.6~23.6cm/sec(2.5~2.9BL cm/sec)로서 개체수가 많을수록 체장당 유영속도가 빠른 것으로 나타났으며, 유속의 변화에 따른 어군의 평균유영속도는 유속이 증가할수록 유영속도도 증가하여 유속이 25cm/sec인 경우에는 유영속도가 30.6 cm/secd(3.8BLcm/sec)에 달하였다. 어군의 평균 유영깊이는 17~38cm로서 주로 중층의 깊이에서 유영하였으며, 유속이 빨라질수록 유영 깊이가 깊어지고 유영 깊이의 변화가 적어졌다. 수조의 벽과 개체 사이의 평균 거리는 17.6~21.4cm였으며, 유속이 빨라질수록 수조의 벽과 개체사이의 거리가 다소 멀어지는 경향을 나타내었다. 개체상호간의 최근거리는 개체수가 많을수록 가까워지며, 유속의 변화에 따른 개체상호간의 최근접거리 평균치는 3.0~5.9cm(0.4~0.7BL cm)로 나타났다. 어군의 3차원적 구조는 군을 형성하는 개체수가 증가함에 따라 군의 형상이 길이, 폭 및 깊이의 모든 방향으로 넓어지며, 유속이 빨라질수록 길이방향과 폭 방향의 크기가 깊이 방향에 비해 상대적으로 커져 각 방향의 평균 상태비는 2.8 : 2.7 : 1이었다.

• International Journal of Contents
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• 제8권4호
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• pp.64-69
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• 2012

The heart is a major dose-limiting organ for radiotherapy of cancer in the thoracic region. The purpose of this study was to examine the protective effect of tranilast on the radiation-induced heart fibrosis model using the C57BL/6 murine strain. A significant reduction in the expression of TGF-${\beta}1$, collagen type I and collagen type III was observed in the radiation plus tranilast group. The authors also suggest the use of tranilast in a clinical trial for the prevention of radiation-induced heart fibrosis.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.426-427
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• 2010

• 한방비만학회지
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• 제16권1호
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• pp.19-26
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• 2016

Objectives: The prevalence of obesity continues rise and obesity and metabolic syndrome is a major problem in global health care. Animal models are used in the drug discovery of novel treatment for obesity. One of common models of obesity is a high fat diet induced obesity in a C5BL/6 mouse, and the development of obesity and glucose tolerance in mouse model is different according to period of diet. Therefore, this study was performed to observe the development of obesity and glucose tolerance during a high fat diet (HFD). Methods: Male C57BL/6 mice, 5 weeks of age, were fed on a standard chow diet as a normal diet (18 kcal% fat) or a HFD (60 kcal% fat) for up to 16 weeks. The various factors related with obesity and insulin resistance were measured at 8, 12, and 16 weeks. Results: The weights of body and epididymal fat were gradually increased for 8~16 weeks, however the change of hyperglycaemia and glucose tolerance have shown different with that of body weight. Blood glucose, oral glucose tolerance and insulin tolerance were increased more clearly at week 12 and 16 than week 8. Lipid accumulation of liver and body temperature were also significantly increased at week 16, compared with normal group. Conclusions: The developments of obesity and related factors were different by a HFD period in a C57BL/6 obese mice. This result suggests that the development of obesity with glucose tolerance and liver lipid may induce clearly by a HFD for 16 weeks.

• Journal of Acupuncture Research
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• 제26권4호
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• pp.49-58
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• 2009

목적 : 파킨슨 병은 기저핵 흑질의 치밀부에서 도파민성 신경세포의 퇴행으로 인하여 발생하는 질병으로 신경 염증이 주요 병인으로 밝혀져 있다. 이 연구는 MPTP 유발 파킨슨 병 동물 모델에서 신수혈($BL_{23}$)에 대한 봉독 약침의 항염증 효과 및 그 기전을 확인하기 위해 시행되었다. 방법 : $C57_{BL}$/6쥐를 무처치군, MPTP+saline군, MPTP+BVA(0.06mg/kg)군, MPTP+BVA(0.6mg/kg)군의 4군으로 나눈 뒤 무처치군을 제외한 모든 그룹에 총 8시간 동안 2시간 간격으로 MPTP-HCl(20mg/kg per dose$\times$4)을 복강내로 주입하였다. MPTP+BVA 군에서 봉독약침은 마지막 MPTP 주입 2시간 후부터 48시간 간격으로 신수혈($BL_{23}$)에 양측으로 각 20${\mu}\ell$씩 주입하였고 MPTP+saline군에서는 봉독약침 대신 Saline을 주입하였다. 마지막 MPTP 주입 후 7일째에 쥐의 뇌를 적출한 후 면역조직화학법을 시행하였다. 결과 : MPTP 유발 파킨슨 병 동물 모델에서 신수혈에 대한 봉독약침은 농도 의존적으로 TH-Immunoreactivity neuron의 감소와 microglial activation을 억제하였다. HSP70-IR neuron은 모든 군에서 나타나지 않았다. 결론 : 봉독약침이 용량의존적으로 microglial activation을 억제하는 효과를 통해 도파민성 신경세포의 파괴를 억제함으로써 항염 효과를 나타냄을 알 수 있었다. 이 결과는 봉독약침이 microglial activation 억제를 통해 임상적으로 파킨슨 병과 같은 신경 퇴행성 질병에 있어 유용한 치료수단이 될 수 있음을 시사한다.

• Journal of Acupuncture Research
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• 제27권5호
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• pp.25-34
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• 2010

Objectives : The purpose of this study is to investigate that effect of Angelica gigas NAKAI pharmacopuncture(AGN-Ph) by concentration at $ST_{36}$(足三里) and $BL_{23}$(腎兪) in Freund's adjuvant rats. Methods : The experimental model of arthritis was induced by injection of Freund's adjuvant into Sprague Dawley(SD) rats. After arthritis was induced, AGN-Ph was injected by concentration at $ST_{36}$ and $BL_{23}$ of rats every other day for 6 times. Thereafter, edema rate, body weight, IFN-${\gamma}$, TNF-${\alpha}$, hematologic assay were measured. Results : The results were as follows, 1. After 3 times AGN-Ph treatment, the mean of edema rate was significantly decreased in AGN-Ph group 3 than control group. And after 6 times AGN-Ph treatment, the mean of edema rate was significantly decreased in AGN-Ph group 1, 2, 3 than control group. 2. The mean of body weight was significantly increased in AGN-Ph group 1, 3 than control group and saline group. 3. The mean of IFN-${\gamma}$ was significantly increased in AGN-Ph group 3 than control group. 4. The mean of TNF-${\alpha}$ was significantly increased in saline group than control group. But the mean of TNF-${\alpha}$ in AGN-Ph group 2, 3 showed no significance compared with control group's. 5. In hematologic assay, levels of WBC, RBC, Hemoglobin, Hematocrit showed no significance in all groups. Conclusions : These results are suggest that the Angelica gigas NAKAI pharmacopuncture(AGN-Ph) at $ST_{36}$ and $BL_{23}$ has a suppressing inflammation effect on Freund's adjuvant arthritis in rats.

• Journal of Veterinary Science
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• 제23권3호
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• pp.41.1-41.15
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• 2022

Background: Proliferative enteritis caused by Lawsonia intracellularis undermines the economic stability of the swine industry worldwide. The development of cost-effective animal models to study the pathophysiology of the disease will help develop strategies to counter this bacterium. Objectives: This study focused on establishing a model of gastrointestinal (GI) infection of L. intracellularis in C57BL/6 mice to evaluate the disease progression and lesions of proliferative enteropathy (PE) in murine GI tissue. Methods: We assessed the murine mucosal and cell-mediated immune responses generated in response to inoculation with L. intracellularis. Results: The mice developed characteristic lesions of the disease and shed L. intracellularis in the feces following oral inoculation with 5 × 10⁷ bacteria. An increase in L. intracellularis 16s rRNA and groEL copies in the intestine of infected mice indicated intestinal dissemination of the bacteria. The C57BL/6 mice appeared capable of modulating humoral and cell-mediated immune responses to L. intracellularis infection. Notably, the expression of genes for the vitamin B12 receptor and for secreted and membrane-bound mucins were downregulated in L. intracellularis -infected mice. Furthermore, L. intracellularis colonization of the mouse intestine was confirmed by the immunohistochemistry and western blot analyses. Conclusions: This is the first study demonstrating the contributions of bacterial chaperonin and host nutrient genes to PE using an immunocompetent mouse model. This mouse infection model may serve as a platform from which to study L. intracellularis infection and develop potential vaccination and therapeutic strategies to treat PE.

• Journal of Acupuncture Research
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• 제18권1호
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• pp.29-39
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• 2001

Purpose : It has been reported that Oyo-Tang has an effect on allergic asthma clinically. In order to investigate the herbal acupuncture solution of the Oyo-Tang on allergic asthma, the Oyo-Tang herbal-acupuncture(OTHA) was applied on BL13 and ST36 of allergic asthma model of the rats. Methods : Then the concentration of serum IgE and the ratio of CD4+ and CD8+ T-cells in peripheral blood were measured and compared with control group. Results : The results obtained as follows; 1. The concentration of serum IgE was decreased significantly in both of the OTHA on BL13 group and on ST36 group as compared to control group. 2. The ratio of CD4+ and CD8+ T-cells in peripheral blood was not different statistically between the normal, the control, the OTHA on BL13 and the OTHA on ST36 group.