• Laboraroty Animal Research
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• v.34 no.4
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• pp.295-301
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• 2018

Nucleotide-binding domain 1 (Nod1) is a cytosolic receptor that is responsible for the recognition of a bacterial peptidoglycan motif containing meso-diaminophimelic acid. In this study, we sought to identify the role of Nod1 in host defense in vivo against pulmonary infection by multidrug resistant Acinetobacter baumannii. Wildtype (WT) and Nod1-deficient mice were intranasally infected with $3{\times}10^7CFU$ of A. baumannii and sacrificed at 1 and 3 days post-infection (dpi). Bacterial CFUs, cytokines production, histopathology, and mouse ${\beta}$-defensins (mBD) in the lungs of infected mice were evaluated. The production of cytokines in response to A. baumannii was also measured in WT and Nod1-deficient macrophages. The bacterial clearance in the lungs was not affected by Nod1 deficiency. Levels of IL-6, $TNF-{\alpha}$, and $IL-1{\beta}$ in the lung homogenates were comparable at days 1 and 3 between WT and Nod1-deficient mice, except the $TNF-{\alpha}$ level at day 3, which was higher in Nod1-deficient mice. There was no significant difference in lung pathology and expression of mBDs (mBD1, 2, 3, and 4) between WT and Nod1-deficient mice infected with A. baumannii. The production of IL-6, $TNF-{\alpha}$, and NO by macrophages in response to A. baumannii was also comparable in WT and Nod1-deficient mice. Our results indicated that Nod1 does not play an important role in host immune responses against A. baumannii infection.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.1
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• pp.69-85
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• 2019

BeiDou navigation satellite system (BDS) is one of the four main types of global navigation satellite systems. The current system has been widely used by the military and by the aerospace, transportation, and marine fields, among others. However, challenges still remain in the BeiDou system, which requires rapid responses for delay-sensitive devices. A differential positioning algorithm called the data center-based differential positioning (DCDP) method is widely used to avoid the influence of errors. In this method, the positioning information of multiple base stations is uploaded to the data center, and the positioning errors are calculated uniformly by the data center based on the minimum variance or a weighted average algorithm. However, the DCDP method has high delay and overload risk. To solve these problems, this paper introduces edge computing to relieve pressure on the data center. Instead of transmitting the positioning information to the data center, a novel method called edge computing-based differential positioning (ECDP) chooses the nearest reference station to perform edge computing and transmits the difference value to the mobile receiver directly. Simulation results and experiments demonstrate that the performance of the ECDP outperforms that of the DCDP method. The delay of the ECDP method is about 500ms less than that of the DCDP method. Moreover, in the range of allowable burst error, the median of the positioning accuracy of the ECDP method is 0.7923m while that of the DCDP method is 0.8028m.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.77-77
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• 2020

Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer mechanisms are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins (XIAP, cIAP-1, survivin), depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and -9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent.

• Journal of Advanced Navigation Technology
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• v.26 no.6
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• pp.418-426
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• 2022

Unlike OSR(observation space representation), the SSR(state space representation) augmentation system is suitable for a one-way broadcasting service because it provides the same corrections to all users in the service area. Due to this advantage, several GNSS(global navigation system) systems such as Galileo, BDS(beidou navigation satellite system), QZSS(quasi zenith satellite system) are establishing PPP (precise point positioning)/PPP-RTK precision positioning services based on SSR messages. Therefore, in this paper, we try to understand the trends of satellite-based PPP/PPP-RTK correction services by analyzing the system configurations, characteristics, and precise positioning performance of satellite-based SSR correction broadcasting services.

• Journal of Cadastre & Land InformatiX
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• v.50 no.1
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• pp.107-123
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• 2020

With stabilization of the recent multi-GNSS infrastructure, and as multi-GNSS has been proven to be effective in improving the accuracy of the positioning performance in various industrial sectors. In this study, in view that SF(Single frequency) GNSS receivers are widely used due to the low costs, evaluate effectiveness of SF Real Time Point Positioning(SF-RT-PP) based on four multi-GNSS surveying methods with RTCM-SSR correction streams in static and kinematic modes, and also derive response challenges. Results of applying SSR correction streams, CNES presented good results compared to other SSR streams in 2D coordinate. Looking at the results of the SF-RT-PP surveying using SF signals from multi-GNSS, were able to identify the common cause of large deviations in the altitude components, as well as confirm the importance of signal bias correction according to combinations of different types of satellite signals and ionospheric delay compensation algorithm using undifferenced and uncombined observations. In addition, confirmed that the improvement of the infrastructure of Multi-GNSS allows SF-RT-SPP surveying with only one of the four GNSS satellites. In particular, in the case of code-based SF-RT-SPP measurements using SF signals from GPS satellites only, the difference in the application effect between broadcast ephemeris and SSR correction for satellite orbits/clocks was small, but in the case of ionospheric delay compensation, the use of SBAS correction information provided more than twice the accuracy compared to result of the Klobuchar model. With GPS and GLONASS, both the BDS and GALILEO constellations will be fully deployed in the end of 2020, and the greater benefits from the multi-GNSS integration can be expected. Specially, If RT-ionospheric correction services reflecting regional characteristics and SSR correction information reflecting atmospheric characteristics are carried out in real-time, expected that the utilization of SF-RT-PPP survey technology by multi-GNSS and various demands will be created in various industrial sectors.

• Journal of the Korean Society for Library and Information Science
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• v.57 no.3
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• pp.325-350
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• 2023

This study focuses on the impact of research data metadata quality evaluation index on the revitalization of K-BDS, a research data platform in the bio field, and examines the mediating effect of motivation factors for utilizing the platform. The investigation employs a structural equation model analysis and bootstrap analysis to explore the interrelationships among the three variables. The findings demonstrate that researchers who prioritize the quality of metadata display higher motivation to use the research data platform, leading to an intention to activate the platform. The study also confirms the mediating effect of motivation factors. Moreover, a comprehensive understanding of the sub-factors within each variable is attained through regression analysis and Sobel test. The results highlight that enhancing searchability is crucial to activate research data sharing in the bio field, while improving discoverability is vital for research data reuse. Interestingly, the study reveals that citationability does not significantly impact platform activation. As a conclusion, to foster platform activation, it is imperative to provide systematic support by enhancing metadata quality. This improvement can not only increase trust in the platform but also institutionally solidify the benefits of citation.

• Korean Journal of Plant Resources
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• v.33 no.4
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• pp.279-286
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• 2020

Purple loosestrife-Lythrum anceps (Koehne) Makino is a herbaceous perennial plant belonging to the Lythraceae family. It has been used for centuries in Korea and other Asian traditional medicine. It has been showed pharmacological effects, including anti-oxidant and anti-microbial effects. However, the mechanisms underlying its anti-cancer effect are not yet understood. In this study, we investigated the mechanism of apoptosis signaling pathways by ethanol extract of Lythrum anceps (Koehne) Makino (ELM) in human leukemia U937 cells. Treatment with ELM significantly inhibited cell growth in a dose-dependent manner by inducing apoptosis, as evidenced by the formation of apoptotic bodies (ApoBDs), DNA fragmentation and increased populations of sub-G1 ratio. Induction of apoptosis by ELM was connected with up-regulation of death receptor (DR) 4 and DR5, pro-apoptotic Bax protein expression and down-regulation of anti-apoptotic Bcl-2 protein, and inhibitor of apoptosis protein (IAP) family proteins, depending on dosage. This induction was associated with Bid truncation, mitochondrial dysfunction, proteolytic activation of caspases (-3, -8 and -9) and cleavage of poly(ADP-ribose) polymerase protein. Therefore, our data indicate that ELM suppresses U937 cell growth by activating the intrinsic and extrinsic apoptosis pathways, and thus may have applications as a potential source for an anti-leukemic chemotherapeutic agent.