• Title/Summary/Keyword: B10

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The Effect of Sihosogansangagambang (SS) on Melanin Synthesis and gene expression in B16F10 Mouse Melanoma Cell (시호소간산가감방(柴胡疎肝散加減方)이 멜라닌 생성 및 유전자발현에 미치는 영향)

  • Kim, Ju-Young;Lim, Hyun-Jung;Shin, Sun-Mi;Yoo, Dong-Youl
    • The Journal of Korean Obstetrics and Gynecology
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    • v.22 no.1
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    • pp.95-109
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    • 2009
  • Purpose: This study was performed to determine the inhibitory effect of Sihosogansangagambang (SS) on melanin synthesis in B16F10 melanoma cells (B16F10). Methods: The inhibitory effects of Sihosogansangagambang on melanin synthesis were used by in vitro assay. To elucidate inhibitory effects of SS on melanin synthesis, we determined the melanin release in B16F10. And to investigate the mechanism of inhibitory effect of SS, we assessed the gene expression of tyrosinase, TRP-1, TRP-2 and ERK-1 in B16F10. Results: 1. SS decreased the release of melanin in B16F10 melanoma cells. 2. SS inhibited mushroom tyrosinase activity in vitro. 3. SS decreased the expression of tyrosinase, TRP-2 in B16F10 melanoma cells, but did not decreased the expression of TRP-1 in B16F10 melanoma cells. 4. SS decreased the expression of ERK-1 in B16F10 melanoma cells. Conclusion: From these results, it may be suggested that SS is possesed of the antimelanogenetic effects.

Genomics-based Sensitive and Specific Novel Primers for Simultaneous Detection of Burkholderia glumae and Burkholderia gladioli in Rice Seeds

  • Lee, Chaeyeong;Lee, Hyun-Hee;Mannaa, Mohamed;Kim, Namgyu;Park, Jungwook;Kim, Juyun;Seo, Young-Su
    • The Plant Pathology Journal
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    • v.34 no.6
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    • pp.490-498
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    • 2018
  • Panicle blight and seed rot disease caused mainly by Burkholderia glumae and Burkholderia gladioli is threatening rice cultivation worldwide. The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent cross-reactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and $3.86{\times}10^2cells$ for B. glumae and $5.85{\times}10^2cells$ for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with a detection limit as low as $1.86{\times}10^3cells$ per rice seed for B. glumae and $1.04{\times}10^4cells$ per rice seed of B. gladioli. The novel primers maybe valuable as a more sensitive, specific, and robust tool for the efficient simultaneous detection of B. glumae and B. gladioli on rice seeds, which is important in combating rice panicle blight and seed rot by early detection and confirmation of the dissemination of pathogen-free rice seeds.

Influential Factor of Life Satisfaction based on Types of Baby Boomer Generation (베이비부머세대의 유형별 삶의 만족 영향 요인)

  • Lee, Hyoung-Ha
    • Journal of Digital Convergence
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    • v.15 no.10
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    • pp.19-29
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    • 2017
  • The purpose of this study was to identify the multidimensional factors that affect the satisfaction of life by type of baby boomer generation. The subjects of this study were 1,895 households who were born from 1955 to 1963 among the respondents in the Korea welfare panel (the 10th year). The baby boomer generation was classified into four types such as gender, income status, male and female as socioeconomic status. The male type IV (B=.074, t=1.992, p<.05), health status (B=.233, t=7.268, p<.001), household income (B=.174, t=4.476, p<.001), depression (B=-.182, t=-5.242, p<.001), self-esteem (B=.255, t=7.027, p<.001), trust (B=.064, t=2.036, p<.05), etc. were found to have a significant effect on the life satisfaction of the male baby boomer generation. The female type IV (B=.100, t=2.598, p<.05), health status (B=.239, t=6.790, p<.001) (P<.001), depression (B=-.181, t=-5.142, p<.001), and self-esteem (B=.320, t=8.600, p<.001), etc. were found to have a significant effect on the life satisfaction of the female baby boomer generation.

'$B_{6\sigma}$ 수명' 척도의 성질

  • Kim Cheol
    • Proceedings of the Korean Reliability Society Conference
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    • 2005.06a
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    • pp.141-145
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    • 2005
  • Nowadays, most industries take $B_{6\sigma}$ quality level as a goal of the ultimate quality level of their products. On the other hand, $B_{10}$ to life indicates the time that $10\%$ of products are failed. There is no relation between the $B_{6\sigma}$ quality level and the $B_{10}$ to life. Therefore, some industries perform their quality control activities and reliability engineering activities separately. So I propose one measure which can express quality and reliability level simultaneously for the products to pursue quality and reliability activities together in the industry.

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Phase Transformation and Reversible Shape Memory Effect of Ti-Ni-Cu Alloys (Ti-Ni-Cu 합금의 상변태 및 가역형상기억효과)

  • Hong, S.W.;Lee, O.Y.;Kim, D.K.
    • Journal of the Korean Society for Heat Treatment
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    • v.5 no.3
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    • pp.149-156
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    • 1992
  • Transformation behavior and reversible shape memory effct of Ti-Ni-Cu alloys with various Cu content has been investigated by means of electrical resistivity measurement, differential scanning calorimetry. X-ray diffraction and strain gage sensor. The transformation sequence in Ti-Ni-Cu alloys substituted by Cu for Ni up to 5at.% occurs to $B2{\leftrightarrow}B19^{\prime}$ and it proceeds in two stages by addition of 10 at.%Cu. i.e. $B2{\leftrightarrow}B19{\leftrightarrow}B19^{\prime}$. But the content of Cu increases up to 20at.%, it has been transformed in one stage ; $B2{\leftrightarrow}B19$. The shape change of Ti-40Ni-10Cu alloy which was constrain aged in circular form bended in $B2{\leftrightarrow}B19$ transformation but it spreaded out in $B19{\leftrightarrow}B19^{\prime}$ transformation. The amount of reversible shape change (${\Delta}{\varepsilon}$) of Ti-47Ni-3Cu alloy constrain aged at $400^{\circ}C$ after solution treatment has a maximum value of about $5.6{\times}10^{-3}$, but that of cold rolled and constrain aged specimens exhibits a little value independent of Cu concentrations.

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$MgB_2$ Sheets using Mixture of Mg and B Powders by Powder Roll Compaction (Mg과 B 혼합분말을 이용하여 분말압연 공정으로 제조된 $MgB_2$ 초전도 판재연구)

  • Chung, K.C.;Chang, S.H.;Sinha, B.B.;Kim, J.H.;Dou, S.X.
    • Progress in Superconductivity
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    • v.13 no.3
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    • pp.184-188
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    • 2012
  • $MgB_2$ superconducting sheets have been fabricated by powder rolling method using mixture of Mg and B powders. Sheet-type $MgB_2$ bulk samples of ~10 mm width and 50-100 mm long were squeezed out after compacted by two rotating rolls of 130 mm diameter with gap distance of 0.5 mm and speed of ~40 cm/min (~1 rpm). The nominal composition of Mg, which is ductile metal, was added up to 30% to facilitate forming the $MgB_2$ sheets. The annealed samples at $900^{\circ}C$ and 3 hrs showed superconducting transition temperature of ~32 K and critical current densities at zero fields were ${\sim}10^5A/cm^2$ at 5 K and ${\sim}5{\times}10^4A/cm^2$ at 20 K.

Upregulation of NF-κB upon differentiation of mouse embryonic stem cells

  • Kim, Young-Eun;Kang, Ho-Bum;Park, Jeong-A;Nam, Ki-Hoan;Kwon, Hyung-Joo;Lee, Young-Hee
    • BMB Reports
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    • v.41 no.10
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    • pp.705-709
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    • 2008
  • NF-${\kappa}B$ is a transcriptional regulator involved in many biological processes including proliferation, survival, and differentiation. Recently, we reported that expression and activity of NF-${\kappa}B$ is comparatively low in undifferentiated human embryonic stem (ES) cells, but increases during differentiation. Here, we found a lower expression of NF-${\kappa}B$ p65 protein in mouse ES cells when compared with mouse embryonic fibroblast cells. Protein levels of NF-${\kappa}B$ p65 and relB were clearly enhanced during retinoic acid-induced differentiation. Furthermore, increased DNA binding activity of NF-${\kappa}B$ in response to TNF-$\alpha$, an agonist of NF-${\kappa}B$ signaling, was seen in differentiated but not undifferentiated mouse ES cells. Taken together with our previous data in human ES cells, it is likely that NF-${\kappa}B$ expression and activity of the NF-${\kappa}B$ signaling pathway is comparatively low in undifferentiated ES cells, but increases during differentiation of ES cells in general.

De novo transcriptome sequencing and gene expression profiling with/without B-chromosome plants of Lilium amabile

  • Park, Doori;Kim, Jong-Hwa;Kim, Nam-Soo
    • Genomics & Informatics
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    • v.17 no.3
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    • pp.27.1-27.9
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    • 2019
  • Supernumerary B chromosomes were found in Lilium amabile (2n = 2x = 24), an endemic Korean lily that grows in the wild throughout the Korean Peninsula. The extra B chromosomes do not affect the host-plant morphology; therefore, whole transcriptome analysis was performed in 0B and 1B plants to identify differentially expressed genes. A total of 154,810 transcripts were obtained from over 10 Gbp data by de novo assembly. By mapping the raw reads to the de novo transcripts, we identified 7,852 differentially expressed genes (log2FC > |10|), in which 4,059 and 3,794 were up-and down-regulated, respectively, in 1B plants compared to 0B plants. Functional enrichment analysis revealed that various differentially expressed genes were involved in cellular processes including the cell cycle, chromosome breakage and repair, and microtubule formation; all of which may be related to the occurrence and maintenance of B chromosomes. Our data provide insight into transcriptomic changes and evolution of plant B chromosomes and deliver an informative database for future study of B chromosome transcriptomes in the Korean lily.

A Design of Ultra Wide Band Single-to-Differential Gain Controlled Low Noise Amplifier Using 0.18 um CMOS (0.18 um CMOS 공정을 이용한 UWB 단일 입력-차동 출력 이득 제어 저잡음 증폭기 설계)

  • Jeong, Moo-Il;Choi, Yong-Yeol;Lee, Chang-Suk
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.3
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    • pp.358-365
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    • 2008
  • A differential-gain-controlled LNA is designed and implemented in 0.18 um CMOS technology for $3.1{\sim}4.8GHz$ UWB system. In high gain mode, measurements show a differential power gain of $14.1{\sim}15.8dB,\;13.3{\sim}15dB$, respectably, an input return loss higher then 10dB, an input IP3 of -19.3 dBm, a noise figure of $4.85{\sim}5.09dB$, while consuming only 19.8 mW of power from a 1.8V DC supply. In low gain mode, measurements show a differential power gain of $-6.1{\sim}-4.2dB,\;-7.6{\sim}-5.6dB$, respectably, an input return loss higher then 10dB, an input IP3 of -1.45 dBm, a noise figure of $8.8{\sim}10.3dB$, while consuming only 5.4mW of power from a 1.8V DC supply.

Nucleolar translocalization of GRA10 of Toxoplasma gondii transfectionally expressed in HeLa cells

  • Ahn, Hye-Jin;Kim, Sehra;Nam, Ho-Woo
    • Parasites, Hosts and Diseases
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    • v.45 no.3
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    • pp.165-174
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    • 2007
  • Toxoplasma gondii GRA10 expressed as a GFP-GRA10 fusion protein in HeLa cells moved to the nucleoli within the nucleus rapidly and entirely. GRA10 was concentrated specifically in the dense fibrillar component of the nucleolus morphologically by the overlap of GFP-GRA10 transfection image with IFA images by monoclonal antibodies against GRA10 (Tg378), B23 (nucleophosmin) and C23 (nucleolin). The nucleolar translocalization of GRA10 was caused by a putative nucleolar localizing sequence (NoLS) of GRA10. Interaction of GRA10 with TATA-binding protein associated factor 1B (TAF1B) in the yeast two-hybrid technique was confirmed by GST pull-down assay and immunoprecipitation assay. GRA10 and TAF1B were also co-localized in the nucleolus after co-transfection. The nucleolar condensation of GRA10 was affected by actinomycin D. Expressed GFP-GRA10 was evenly distributed over the nucleoplasm and the nucleolar locations remained as hollows in the nucleoplasm under a low dose of actinomycin D. Nucleolar localizing and interacting of GRA10 with TAF1B suggested the participation of GRA10 in rRNA synthesis of host cells to favor the parasitism of T. gondii.