• Title/Summary/Keyword: B&BR

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Comparison of Aviary, Barn and Conventional Cage Raising of Chickens on Laying Performance and Egg Quality

  • Ahammed, M.;Chae, B.J.;Lohakare, J.;Keohavong, B.;Lee, M.H.;Lee, S.J.;Kim, D.M.;Lee, J.Y.;Ohh, S.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.8
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    • pp.1196-1203
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    • 2014
  • This study intended to compare the productive performance of three different layer raising systems; conventional cage (CC), barn (BR) and aviary (AV). The AV is welfare bestowed housing that allows free locomotion for birds within the BR. The BR allows bird's free locomotion inside BR but without multilevel structures. Both pullets and cockerels were housed together in both AV and BR, but only pullets in CC. Seventeen weeks old Lohmann Brown Lite (n = 800) pullets were housed in AV during this study. The same age layer pullets were simultaneously assigned to either at CC or BR to compare egg production performance with AV. The duration of experiment was 40 weeks (from 21st to 60th week). There were no remarkable differences in egg production, hen day egg production (HDEP) and average egg weight among three rearing systems. First 20 weeks (phase-1) average HDEP (%) of AV, CC, and BR were 85.9, 88.8, 87.1 and average egg weights (g) were 57.5, 59.9, and 56.9 respectively. Those of the remaining 20 weeks (phase-2) were 87.1, 87.9, 85.5 and 64.2, 63.0 62.1, respectively. Daily feed intakes (122 g, 110 g, 125 g); feed conversion ratio (2.4, 2.1, 2.5) and daily egg mass (53.9 g, 54.4 g, 52.8 g) data from AV, CC, and BR were not influenced significantly by the respective raising systems. Daily feed intake of layers in both AV (124 g) and BR (127 g) tended to be higher than that in CC (113 g) during phase-2. Overall, exterior egg quality (dirty and cracked eggs) in both phases was superior in BR compared with AV and CC, whereas CC generated intermediate results. This study indicated that the HDEP per se in AV and BR were not significantly different from that in CC. The study implied that the facility depreciation cost for AV and cost for increased feed intake in AV compared to CC are believed to be critical to evaluate the cost effectiveness of egg production in AV.

Enhancement of cadmium resistance by overexpression of BrMT3 in Arabidopsis (BrMT3 고발현에 의한 애기장대의 카드뮴 저항성 증진)

  • Kim, Sun-Ha;Song, Won-Yong;Ahn, Young-Ock;Lee, Haeng-Soon;Kwak, Sang-Soo;Choi, Kwan-Sam
    • Journal of Plant Biotechnology
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    • v.36 no.1
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    • pp.68-74
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    • 2009
  • We have previously demonstrated that overexpression and characterization of Brassica rapa type-l metallothionein gene (BrMT1) in Arabidopsis which showed enhanced resistance to cadmium and ROS. Here, we present the consistent study of our previous report about BrMTs. BrMT3 expressing DTY167 cells showed resistance to Zn and Pb as well as Cd. Thus, we have developed the BrMT3 overexpression Arabidopsis to enhance capacity for metal stresses. Successful expression and localization were achieved using the rubisco transit peptides of RbcS-BrMT3-GFP protein, which was confirmed by western blot analysis with the GFP antibody and green fluorescence signal from the chloroplast. BrMT3 overexpression Arabidopsis plants exhibited a higher resistance to cadmium compared to control plants. This result indicates that BrMT3 would be applicable to the development of plants with enhanced resistance against heavy metal stresses.

Study on the Isomerization and Dehydrobromination Mechanism of Brominated Butyl Rubber (브롬화된 부틸고무의 탈브롬화 밑 이성질체화 메커니즘에 대한 연구)

  • Wu, Yibo;Guo, Wenli;Li, Shuxin;Gong, Liangfa;Shang, Yuwei
    • Polymer(Korea)
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    • v.34 no.1
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    • pp.69-73
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    • 2010
  • Effects of reaction time and temperature on the isomerization and dehydrobromination reactions of brominated butyl rubber were investigated. The structural composition of brominated butyl rubber was determined by Fourier transform infrared spectroscopy (FT-IR) and proton nuclear magnetic resonance spectroscopy($^1H$-NMR), Density functional theory (DFT) was used to study on the isomerization and dehydrobromination mechanisms of model compounds. The geometries for model compounds of 3-bromo-5,5,7,7-tetramethyl-2(2',2',4',4'-tetramethyl)pentyl-1-octylene (3BrOE), 1-bromo-5,5,7,7-tetramethyl-2(2',2',4',4'-tetramethyl)pentyl-2-octylene (1Br2OE) and 5,5,7,7-tetramethyl-2(2',2', 4',4'-tetramethyl)pentyl-1,3-octadiene (CD) had been optimized by using density functional theory at B3LYP/3-21G and B3LYP/6-31G levels. The predicted energy of 3BrOE lies higher than that of 1Br2OE which suggests that 1Br2OE configuration is more stable than the 3BrOE configuration. Compared with the energy barrier, the pathway of dehydrobromination is less competitive than that of isomerization. This is qualitatively consistent with the experimental results.

Isolation and Structure of cis,fac -Dibromooxotris(2,6-dimethylphenyl isocyanide)molybdenum(IV), cis,fac-$[Mo(O)Br_2(CN-C_6H_3-2,6-Me_2)_3]$ (cis,fac-Dibromooxotris(2,6- dimethylphenyl isocyanide)molybdenum (IV), cis,fac-$[Mo(O)Br_2(CN-C_6H_3-2,6-Me_2)_3]$의 분리 및 구조)

  • 이범준;한원석;이순원
    • Korean Journal of Crystallography
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    • v.13 no.2
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    • pp.82-85
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    • 2002
  • From the reaction of cis,cis,trans- [MoBr/sub 2/(CO)/sub 2/(PPh/sub 3/)/sub 2/]with 2,6-dimethylphenyl isocyanide, a molybdenum oxohaloisocyanide compound cis,fac-[Mo(O)Br/sub 2/,(CN-C/sub 6/H/sub 3/,-2,6-Me/sub 2/)sub 3/] (1) was iso-lated. Compound 1 was characterized by spectroscopy (/sup 1/H-NMR, /sup 13/C{/sup 1/H}-NMR, IR) and X-ray diffraction. Crystallographic data for 1: triclinic space group P(equation omitted), a=9.172(2) (equation omitted), b = 11.550(3) (equation omitted), c = 15.106(3) (equation omitted), α = 100.44(2)°, β= 107.12(2)°, γ= 107.83(1)°, Z = 2, R(wR/sub 2/) = 0.0529(0.1344).

Characterization and Gene Co-expression Network Analysis of a Salt Tolerance-related Gene, BrSSR, in Brassica rapa (배추에서 염 저항성 관련 유전자, BrSSR의 기능 검정 및 발현 네트워크 분석)

  • Yu, Jae-Gyeong;Lee, Gi-Ho;Park, Ji-Hyun;Park, Young-Doo
    • Horticultural Science & Technology
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    • v.32 no.6
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    • pp.845-852
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    • 2014
  • Among various abiotic stress factors, soil salinity decreases the photosynthetic rate, growth, and yield of plants. Recently, many genes have been reported to enhance salt tolerance. The objective of this study was to characterize the Brassica rapa Salt Stress Resistance (BrSSR) gene, of which the function was unclear, although the full-length sequence was known. To characterize the role of BrSSR, a B. rapa Chinese cabbage inbred line ('CT001') was transformed with pSL94 vector containing the full length BrSSR cDNA. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that the expression of BrSSR in the transgenic line was 2.59-fold higher than that in the wild type. Analysis of phenotypic characteristics showed that plants overexpressing BrSSR were resistant to salinity stress and showed normal growth. Microarray analysis of BrSSR over-expressing plants confirmed that BrSSR was strongly associated with ERD15 (AT2G41430), a gene encoding a protein containing a PAM2 motif (AT4G14270), and GABA-T (AT3G22200), all of which have been associated with salt tolerance, in the co-expression network of genes related to salt stress. The results of this study indicate that BrSSR plays an important role in plant growth and tolerance to salinity.

An Experimental Study on Beat and Mass Transfer Characteristics of Helical Absorber (헬리컬 흡수기의 열ㆍ물질전달 특성에 관한 실험적 연구)

  • Kwon, Oh-Kyung;Yun, Jae-Ho;Yoon, Jung-In
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.28 no.1
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    • pp.81-88
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    • 2004
  • In this study, heat and mass transfer characteristics of five components solution (LiBr+Lil+LiNO$_3$+LiCl+$H_2O$) which could be substituted for commonly used LiBr solution are tested using a helical absorber. The arrangement of helical-typed heat exchangers allows to make the system more compact as compared to conventional one. The effects of experimental parameters, such as the solution flow rate, cooling water, solution temperature, solution concentration and surfactant have been investigated in view of the heat and mass transfer. The results of the experiment of heat and mass transfer performance show that five components solution should have 2% higher concentration fur equal absorption capacity of LiBr solution. But considering that five components solution have higher solubility than LiBr solution about 4% high concentration, five components solution could be applied to a small sized water cooled or air cooled absorption chiller/heater. The increase of heat and mass transfer coefficient by surfactant addition is about 25∼30% and 23∼40% respectively.

An Experimental Study on Flow and Heat / Mass Transfer Characteristics of $LiBr-H_2O$ Solution Flowing over a Cooled Horizontal Tube (수평 냉각관 외부를 흘러내리는 $LiBr-H_2O$ 수용액의 유동 및 열/물질 전달 특성에 관한 실험적 연구)

  • Seal, Sin-Su;Lee, Sang-Yang
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.24 no.8
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    • pp.1085-1096
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    • 2000
  • An experimental study was performed to examine the heat and mass transfer characteristics of $LiBr-H_2O$ solution flowing over a single horizontal tube with the water vapor absorption. Effects of the flow rate and the temperature of the solution at the top of the tube, the absorber pressure and the drainage pattern were considered. The absorption rate depends highly on the absorber pressure at the low flow rate condition while on the solution inlet temperature at the high flow rate condition. Also, when the flow rate is low, the absorption performance with the sheet flow drainage appeared to be higher than that with the dripping/jet drainage. However, at the high flow rate condition, the case became reversed. The liquid film became wavy with the higher absorption rate. The waves were more probable to form with the lower flow rate and temperature of the solution, and with the higher absorber pressure.

Cell proliferation and migration mechanism of caffeoylserotonin and serotonin via serotonin 2B receptor in human keratinocyte HaCaT cells

  • Kim, Hye-Eun;Cho, Hyejoung;Ishihara, Atsushi;Kim, Byungkuk;Kim, Okjoon
    • BMB Reports
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    • v.51 no.4
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    • pp.188-193
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    • 2018
  • Caffeoylserotonin (CaS), one derivative of serotonin (5-HT), is a secondary metabolite produced in pepper fruits with strong antioxidant activities. In this study, we investigated the effect of CaS on proliferation and migration of human keratinocyte HaCaT cells compared to that of 5-HT. CaS enhanced keratinocyte proliferation even under serum deficient condition. This effect of CaS was mediated by serotonin 2B receptor (5-HT2BR) related to the cell proliferation effect of 5-HT. We also confirmed that both CaS and 5-HT induced G1 progression via 5-HT2BR/ERK pathway in HaCaT cells. However, Akt pathway was additionally involved in upregulated expression levels of cyclin D1 and cyclin E induced by CaS by activating 5-HT2BR. Moreover, CaS and 5-HT induced cell migration in HaCaT cells via 5-HT2BR. However, 5-HT regulated cell migration only through ERK/AP-1/MMP9 pathway while additional Akt/NF-${\kappa}B$/MMP9 pathway was involved in the cell migration effect of CaS. These results suggest that CaS can enhance keratinocyte proliferation and migration. It might have potential as a reagent beneficial for wound closing and cell regeneration.

Cloning of 17S-Ribosomal RNA Gene from the Hygromycin Resistant Tetrahymena thermophila (Hygromycin내성 Tetrahymena thermophila의 17S-Ribosomal RNA유전자의 Cloning)

  • 홍용기
    • Microbiology and Biotechnology Letters
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    • v.14 no.2
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    • pp.133-137
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    • 1986
  • 17S-ribosomal RNA gene from the hygromycin resistant protozoan Tetrahymena thermophila hmr 3 was cloned on E. coli vector pBR 322 as part of study to work the 17S-rRNA structure and the mechanism of hygromycin resistance. The 17S-rDNA was inserted into the Hind 111 site of pBR 322. The clones having recombinant plasmid were selected by the method of colony hybridization with a 17S-rDNA probe of wild type B1868. The orientation of 17S-rDNA insert was located near the tetracycline resistant gene of pBR 322 in a clone 5-19 with the recombinant plasmid.

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An Experimental Study on the Effect of Low Fin Tube Geometry on Pool Boiling of a LiBr Solution (낮은 핀관의 형상이 LiBr 수용액의 풀 비등에 미치는 영향에 대한 실험적 연구)

  • Kim, Nae-Hyun
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.39 no.7
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    • pp.591-598
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    • 2015
  • Pool boiling heat transfer coefficients of a LiBr solution were obtained for seven low fin tubes having different fin pitch and fin height. The test range covered saturation pressure from 7.38kPa to 101.3kPa, heat flux from $20kW/m^2$ to $40 kW/m^2$ and LiBr concentration from 0% to 50%. The optimum fin geometry for the present experimental range turned out to be 26 fpi with 0.18 mm fin height.The advantage of added heat transfer area and the disadvantage of slower bubble growth and departure appear to have yielded an optimum fin pitch. The heat transfer coefficient decreased as saturation pressure decreased and Libr concentration increased. The reason may be attributed to the low saturation pressure, which increased the bubble departure diameter and decreased the bubble departure frequency. As the LiBr concenreation increased, the saturation temperature increased and the mass diffusion rate decreased, which resulted in a reduced heat transfer coefficient. The heat transfer coefficients of the low fin tube were greater than those of the smooth tube. Correlations were developed based on the present data.