• 한국환경농학회지
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• 제37권4호
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• pp.312-316
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• 2018

BACKGROUND: Yeast biotechnology finds applications in various industries. Hence, we sought to explore the yeasts associated with the flower of Aster spathulifolius Maxim. This study aimed to isolate yeasts from the flower of the plant and screen for biosurfactant-producing yeasts. METHODS AND RESULTS: We collected flowers of Aster spathulifolius Maxim. and performed pure isolation using four types of media. In total, 117 strains belonging to 4 genera, namely, Cryptococcus (75 strains), Aureobasidium pullulans (30 strains), Candida (11 strains), and Rhodotorula (1 strain), were isolated and identified by ITS sequencing. Upon in-depth analysis, Cryptococcus, the most dominant genus (75 strains) was categorized into the 'Unknown group'. Upon in-depth analysis of A. pullulans, we discovered the 'Unknown group I' (27 strains) and the 'Unknown group II' (2 strains), which have not been reported previously. Two A. pullulans isolates with potent surfactant activity were selected via the screening procedure. CONCLUSION: In this study, a total of 117 strains were isolated from the flower of Aster spathulifolius Maxim. In addition, two biosurfactant-producing yeasts were identified from among the isolated yeasts.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.35-35
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• 2019

Aster spathulifolius Maxim. is belongs to the Asteraceae family which is distributed only in Korea and Japan. It is recognize as a traditionally medicinal plants and economically valuable in ornamental field. However, among the Asteraceae family, the Aster genus, which is lacks in genomic resources and information of molecular function. Therefore, we used high throughput RNA-sequencing transcriptome data of the A. spathulifolius to know molecular level function. DeNovo assembly produced 98,660 unigene with N50 value 1126 bp. Unigenes was performed to analyses the functional annotation against NCBI database like plant database of nucleotide (Nt) and non-redundant protein (Nr), Pfam, Uniprot, KEGG and Transcriptional factor (TF). In addition, Distribution of SSR markers also analyzed for future perfectives. Further, Comparing with other two Asteraceae family species like, Karelinia caspica and Chrysanthemum morifolium to the A. spathulifolius shows the number of gene that regulated in internal and external stress respectively salt-tolerant and heat and drought stress to understand the molecular basis related to the different environments stress.

• Archives of Pharmacal Research
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• 제29권10호
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• pp.845-848
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• 2006

Three new sesquiterpene hydroperoxides, 1-[3-(2-hydroperoxy-3-methylbut-3-en)-4-hydroxyphenyl]ethanone (2), $7{\beta}-hydroperoxy-eudesma-11-en-4-ol$ (3), and $7{\alpha}-hydroperoxymanool$ (4), together with three known compounds, germacrone (1), ent-germacra-4(15),5,10(14)-trien-$1{\alpha}$-ol (5) and teucdiol A (6) were isolated from the aerial parts of Aster spathulifolius (Compositae). Their structures were characterized using chemical and spectroscopic methods. The isolated compounds were tested for their cytotoxicity against five human tumor cell lines in vitro using a SRB method. The two new compounds, 3 and 4, showed moderate cytotoxicity against human cancer cells with $ED_{50}$ values ranging from 0.24 to $13.27\;{\mu}g/mL$.

• 한국약용작물학회지
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• 제14권6호
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• pp.347-353
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• 2006

This study was conducted to evaluate the vegetation structure and ordination of the Peucedanum japonicum Thunb. community by the Phytosociological method, floristic composition table on east coast of South Korea. The vegetation of Peucedanum japonicum Thunb. community was classified into 2 communities such as the Aster Spathulifolius community and the calystegia soldanella-Artemisia princeps community. Vegetation of the Peucedanum japonicum Thunb. community with the Aster spathulifolius community was shown southward, whereas vegetation of Peucedanum japonicum Thunb. community with the calystegia soldanella-Artemisia princeps community was shown northward from the base line of Pohang at North latitude of 36" 05'. Accordingly, the Peucedanum japonicum Thunb. community was grown commonly with the Aster spathulifolius community southward of the base line of Pohang, whereas it was grown commonly with the calystegia soldanella-Artemisia princeps community northward on the east coast. All environmental conditions of habitat taken together, the optimum habitat of Peucedanum japonicum Thunb. was at Ulsan geographically and the middle region of sea cliff topography, and was suitable for alkali. sandyloam.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.192.3-193
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• 2003

Aster species has been used in traditional chinese medicine for treatment of a bruies and asthma. On reviewing the literatures of this species, monoterpene glycosides, diterpenoids, triterpene glycosides, cyclic pentapeptides, oligopeptides and flavonoids 1) were isolated and some pharmacological activites were investigated 2). In continuation of our search for bioative components from Korean medicinal plants, we have examined Aster spathulifolius, collected from Jeju island on August 2001. (omitted)

• 한국환경복원기술학회지
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• 제13권1호
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• pp.24-31
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• 2010

This study was carried out to analyze vegetation and soil characteristic, and ordination of Scrophularia takesimensis population, Ulleung Island. The Scrophularia takesimensis population was classified into Aster spathulifolius for. oharai dominant population, Vitis amurensis dominant population and Scrophularia takesimensis typical population. The Scrophularia takesimensis population was located in elevation of 1m to 6m, in Ulleung Island. In the study sites, soil organic matter, nitrogen, available phosphorous, exchangeable potassium, exchangeable calcium, exchangeable magnesium and exchangeable sodium concentration, and cation exchange capacity, and soil pH were 9.1~19.1%, 0.19~0.52%, 87.1~196.7mg/kg, 2.0~2.8cmol+/kg, 5.4~5.9cmol+/kg, 5.9~8.8cmol+/kg, 4.4~4.8cmol+/kg, 20.3~26.7cmol+/kg, and 6.8~8.0, respectively. The Vitis amurensis dominant population was mainly found in the high percentage of total nitrogen, organic matter, CEC in comparison with Aster spathulifolius for. oharai dominant population.

• Plant Resources
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• 제8권3호
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• pp.244-249
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• 2005

The growth charateristics and karyotypes of Aster spathulifolius collected from 5 sites including coastal and island region on the Korean peninsula, were analysed. Several morphological characteristics of the plants such as leaf length, leaf width, top internode, medium internode, spike branching, flower diameter, number of petal, leaf color, leaf form, stem and leaf hair, viscosity, and serration of the plants were distinctly different depending on the native region from which they were collected. Karyotypic analysis showed that the chromosome number was all diploid (2n=18), with one pair of submetacentric satellite chromosomes. The chromosome composition included 7 pairs of metacentric chromosomes and 2 pairs of submetacentric chromosomes in all plants. However, chromosome order and the ranges of the chromosome lengths were a little different from plant to plant according to their native growing regions. The plants from Geoje-Do especially showed large differences in the chromosome lengths between the longest and the shortest compared to the plants from other places. This results provide important data to support the classification of the species into several sub-species.

• Journal of Microbiology and Biotechnology
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• 제23권1호
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• pp.125-130
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• 2013

Influenza viruses cause significant morbidity and mortality in humans through epidemics or pandemics. Currently, two classes of anti-influenza virus drugs, M2 ion-channel inhibitors (amantadin and rimantadine) and neuraminidase inhibitors (oseltamivir and zanamivir), have been used for the treatment of the influenza virus infection. Since the resistance to these drugs has been reported, the development of a new antiviral agent is necessary. In this study, we examined the antiviral efficacy of the plant extracts against the influenza A/PR/8/34 infection. In vitro, the antiviral activities of the plant extracts were investigated using the cell-based screening. Three plant extracts, Thuja orientalis, Aster spathulifolius, and Pinus thunbergii, were shown to induce a high cell viability rate after the infection with the influenza A/PR/8/34 virus. The antiviral activity of the plant extracts also increased as a function of the concentration of the extracts and these extracts significantly reduced the visible cytopathic effect caused by virus infections. Furthermore, the treatment with T. orientalis was shown to have a stronger inhibitory effect than that with A. spathulifolius or P. thunbergii. These results may suggest that T. orientalis has anti-influenza A/PR/8/34 activity.

• Journal of Species Research
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• 제6권1호
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• pp.76-90
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• 2017

Aster altaicus var. uchiyamae Kitam. is an endemic taxon of Korea and is protected by law as an endanger taxon. The genetic information of A. altaicus var. uchiyamae is unavailable in Genbank. Here we sequenced chloroplast genome of A. altaicus var. uchiyamae. The cp-genome of Aster altaicus var. uchiyamae was 152,446 bps in size: LSC was 84,240 bps, IR 25,005 bps, SSC 18,196 bps. The cp-genome contains 112 genes and 21 introns consisted of 79 protein coding genes(PCGs), 4 RNA genes, and 29 tRNA genes, with 20 group II introns and one group I intron. There were three pseudo-genes including ${\psi}$-ycf1, ${\psi}$-rps19, and ${\psi}$-trnT_GGU. Eighteen genes, five introns, and parts of two genes and an intron are found within the IR, which has two copies. The cp-DNA of Aster altaicus var. uchiyamae is distinguished from A. spathulifolius, only known cp-genome of the genus Aster, by 172 SNP in genic regions of 43 PCGs and 21 indels in 11 PCGs and SSU. The chloroplast genome sequence was deposited at GenBank (KX35265).

• 현장농수산연구지
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• 제20권2호
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• pp.31-38
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• 2018

본 연구에서는 보다 효율적인 도시녹화 및 정원식물 생산을 위해 도시녹화 및 정원에 이용되는 주요 초본류 가운데 하나인 해국 유묘에 대해 피트모스 대체재로 바이오차의 가능성을 살펴보고자 바이오차 처리 수준에 따른 해국 유묘의 생육차이를 조사하였다. 연구결과 바이오차를 혼합한 배양토 처리에 따른 해국 유묘의 생장은 피트모스, 버미큘라이트, 펄라이트(1:1:1, v/v), 바이오차, 피트모스, 버미큘라이트, 펄라이트(1:2:3:3, v/v) 처리구에서 우수한 경향을 보였다. 따라서 해국 재배 시 피트모스, 버미큘라이트, 펄라이트(1:1:1, v/v)의 인공용토에서 피트모스를 대체할 만한 생장 효과를 나타낸 처리 비율은 바이오차, 피트모스, 버미큘라이트, 펄라이트(1/3:2/3:1:1, v/v) 처리구로, 사용된 바이오차의 유형, 함께 사용된 상토의 재료, 혼합 비율 등이 영향을 주었을 것으로 사료된다.