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http://dx.doi.org/10.4014/jmb.1210.10074

Antiviral Activity of the Plant Extracts from Thuja orientalis, Aster spathulifolius, and Pinus thunbergii Against Influenza Virus A/PR/8/34  

Won, Ji-Na (Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Lee, Seo-Yong (Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Song, Dae-Sub (Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Poo, Haryoung (Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB))
Publication Information
Journal of Microbiology and Biotechnology / v.23, no.1, 2013 , pp. 125-130 More about this Journal
Abstract
Influenza viruses cause significant morbidity and mortality in humans through epidemics or pandemics. Currently, two classes of anti-influenza virus drugs, M2 ion-channel inhibitors (amantadin and rimantadine) and neuraminidase inhibitors (oseltamivir and zanamivir), have been used for the treatment of the influenza virus infection. Since the resistance to these drugs has been reported, the development of a new antiviral agent is necessary. In this study, we examined the antiviral efficacy of the plant extracts against the influenza A/PR/8/34 infection. In vitro, the antiviral activities of the plant extracts were investigated using the cell-based screening. Three plant extracts, Thuja orientalis, Aster spathulifolius, and Pinus thunbergii, were shown to induce a high cell viability rate after the infection with the influenza A/PR/8/34 virus. The antiviral activity of the plant extracts also increased as a function of the concentration of the extracts and these extracts significantly reduced the visible cytopathic effect caused by virus infections. Furthermore, the treatment with T. orientalis was shown to have a stronger inhibitory effect than that with A. spathulifolius or P. thunbergii. These results may suggest that T. orientalis has anti-influenza A/PR/8/34 activity.
Keywords
Plant extract; Influenza A virus; antiviral activity; cytopathic effect;
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  • Reference
1 CDC. 2009. Effectiveness of 2008-09 trivalent influenza vaccine against 2009 pandemic influenza A (H1N1) - United States, May-June 2009. Morb. Mortal. Wkly. Rep. 58.
2 Bright, R. A., D. K. Shay, B. Shu, N. J. Cox, and A. I. Klimov. 2006. Adamantane resistance among influenza A viruses isolated early during the 2005-2006 influenza season in the United States. JAMA 295: 891-894.   DOI   ScienceOn
3 Cassetti, M. C., R. Couch, J. Wood, and Y. Pervikov. 2005. Report of meeting on the development of influenza vaccines with broad spectrum and long-lasting immune responses, World Health Organization, Geneva, Switzerland, 26-27 February 2004. Vaccine 23: 1529-1533.   DOI   ScienceOn
4 Choi, H. J., J. H. Song, K. S. Park, and D. H. Kwon. 2009. Inhibitory effects of quercetin 3-rhamnoside on influenza A virus replication. Eur. J. Pharm. Sci. 37: 329-333.   DOI   ScienceOn
5 Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction. Anal. Biochem. 162: 156-159.
6 Condit, R. C. 2006. Fields virology, pp. 25-57. In D. M. Knipe and P. M. Howley (eds.). Principles of Virology, 5th Ed. Lippincott Williams and Wilkins, Philadelphia.
7 Couch, R. B. and G. G. Jackson. 1976. Antiviral agents in influenza - summary of Influenza Workshop VIII. J. Infect. Dis. 134: 516-527.   DOI   ScienceOn
8 De Clercq, E. 2004. Antiviral drugs in current clinical use. J. Clin. Virol. 30: 115-133.   DOI   ScienceOn
9 Furuta, Y., K. Takahashi, M. Kuno-Maekawa, H. Sangawa, S. Uehara, K. Kozaki, et al. 2005. Mechanism of action of T-705 against influenza virus. Antimicrob. Agents Chemother. 49: 981-986.   DOI   ScienceOn
10 Hui, E. K. and D. P. Nayak. 2001. Role of ATP in influenza virus budding. Virology 290: 329-341.   DOI   ScienceOn
11 Garten, R. J., C. T. Davis, C. A. Russell, B. Shu, S. Lindstrom, A. Balish, et al. 2009. Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science 325: 197-201.   DOI   ScienceOn
12 Glezen, W. P. 1996. Emerging infections: Pandemic influenza. Epidemiol. Rev. 18: 64-76.   DOI   ScienceOn
13 Hampson, A. W. 2002. Influenza virus antigens and 'antigenic drift', pp. 49-85. In C. W. Potter (ed.). Perspectives in Medical Virology, Vol. 7. Elsevier.
14 Irvine, J. D., L. Takahashi, K. Lockhart, J. Cheong, J. W. Tolan, H. E. Selick, and J. R. Grove. 1999. MDCK (Madin-Darby canine kidney) cells: A tool for membrane permeability screening. J. Pharm. Sci. 88: 28-33.   DOI   ScienceOn
15 Jackson, D. C. and L. E. Brown. 1991. A synthetic peptide of influenza virus hemagglutinin as a model antigen and immunogen. Pept. Res. 4: 114-124.
16 Kawaoka, Y. 2006. Influenza Virology: Current Topics. Caister Academic Press.
17 Kelly, H. and K. Grant. 2009. Interim analysis of pandemic influenza (H1N1) 2009 in Australia: Surveillance trends, age of infection and effectiveness of seasonal vaccination. Euro. Surveill. 14.
18 Kiso, M., K. Takahashi, Y. Sakai-Tagawa, K. Shinya, S. Sakabe, Q. M. Le, et al. 2010. T-705 (favipiravir) activity against lethal H5N1 influenza A viruses. Proc. Natl. Acad. Sci. USA 107: 882-887.   DOI   ScienceOn
19 Murris-Espin, M., A. Didier, S. Mezghani, L. Lacassagne, and P. Leophonte. 1999. Influenza and asthma. Rev. Mal. Res. 16: 9-15.
20 Noah, J. W., W. Severson, D. L. Noah, L. Rasmussen, E. L. White, and C. B. Jonsson. 2007. A cell-based luminescence assay is effective for high-throughput screening of potential influenza antivirals. Antiviral Res. 73: 50-59.   DOI   ScienceOn
21 Numazaki, Y., T. Oshima, A. Ohmi, A. Tanaka, Y. Oizumi, S. Komatsu, et al. 1987. A microplate method for isolation of viruses from infants and children with acute respiratory infections. Microbiol. Immunol. 31: 1085-1095.   DOI
22 Palese, S. M. 2007. Orthomyxoviridae: The viruses and their replication, pp. 1647-1689. In K. DM and H. PM (eds.). Fields Virology, 5th Ed. Lippincott Williams &Wilkins, Philadelphia.
23 Poland, G. A., R. M. Jacobson, and P. V. Targonski. 2007. Avian and pandemic influenza: An overview. Vaccine 25: 3057-3061.   DOI   ScienceOn
24 WHO. 2009. Summary report of a high-level consultation: New influenza A (H1N1). World Health Organization, Geneva.
25 Renaud, C., J. Kuypers, and J. A. Englund. 2011. Emerging oseltamivir resistance in seasonal and pandemic influenza A/ H1N1. J. Clin. Virol. 52: 70-78.   DOI   ScienceOn