• Asian-Australasian Journal of Animal Sciences
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• 제22권8호
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• pp.1091-1101
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• 2009

In an attempt to understand the biochemical mechanism for the synthesis of the anabolic steroid, 19-nortestosterone, produced by prepubertal boar testes and its physiological role, normalized complementary DNA (cDNA) from boar testes was generated. DNA sequencing of 2,016 randomly selected clones yielded 794,116 base pairs of high quality nucleotide sequence. Computer-assisted assembly of the nucleotide sequence of each clone resulted in 423 contigs and 403 singletons including several genes for steroidogenic enzymes and molecules related to steroid metabolism. Analysis of gene expression pattern by use of the presently-fabricated cDNA microarray identified a number of genes that were differentially expressed during the postnatal development period in boar testes. Two genes of unknown function were identified to be highly expressed in the testis of 2-weeks-old neonatal boar. In addition, the sequencing of open reading frames of these genes revealed their homology with human alpha hemoglobin and Homo sapiens hypothetical LOC643669, transcript variant 1. Moreover, the transcripts of these genes were also detected in porcine muscle and adipocytes, in addition to Leydig cells of pigs.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.181-188
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• 2016

In this study, we developed 15 novel polymorphic simple sequence repeat (SSR) markers by SSR-enriched genomic library construction from Codonopsis lanceolata. We obtained a total of 226 non-redundant contig sequences from the assembly process and designed primer sets. These markers were applied to 53 accessions representing the cultivated C. lanceolata in South Korea. Fifteen markers were sufficiently polymorphic, and were used to analyze the genetic relationships between the cultivated C. lanceolata. One hundred three alleles of the 15 SSR markers ranged from 3 to 19 alleles at each locus, with an average of 6.87. By cluster analysis, we detected clear genetic differences in most of the accessions, with genetic distance varying from 0.73 to 0.93. Phylogenic analysis indicated that the accessions that were collected from the same area were distributed evenly in the phylogenetic tree. These results indicate that there is no correlative genetic relationship between geographic areas. These markers will be useful in differentiating C. lanceolata genetic resources and in selecting suitable lines for a systemic breeding program.

• Fisheries and Aquatic Sciences
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• 제10권3호
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• pp.119-126
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• 2007

The analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and the development of resources for functional genomics. To analyze the transcriptome of the abalone Haliotis discus hannai, we conducted EST analysis using seven cDNA libraries made from gill, gut, hepatopancreas, skin, muscle, testis, and ovary. Redundant ESTs were assembled into overlapping contiguous sequences using the assembly program ICAtools. We found that the total 1,393 ESTs formed 135 clusters and 951 singletons, indicating that the overall redundancy of the library was 22%. Of the 1,393 clones, BLAST identified 1,278 clones (91.7%) as known genes; 115 clones (8.3%) did not match any previously described gene. Based on the major functions of their encoded proteins, the identified clones were classified into 16 broad categories. Sequence analysis revealed the presence of micro satellite-containing genes that may be valuable for further gene mapping studies. This study contributes to the identification of numerous EST clones that can be applied to further clarifying the genetics and developmental biology of abalone.

• 대한지리학회지
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• 제42권3호
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• pp.434-452
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• 2007

경제의 글로벌화에 따른 자동차 부품산업의 구조 재편과정에서 경쟁력 강화를 위한 기업의 입지선택과 공간조직은 더욱 중요해졌다. 완성차 업체의 인수 합병으로 촉발된 부품업체의 변화는 거래선 다변화와 부품 생산의 모듈화를 중심으로 진행되었다. 이에 따른 부품업체의 입지변화와 생산조직의 공간적 특성은 다음과 같다. 첫째, 전국적인 스케일에서 보면, 수도권 남부 및 충청권 북부지역에서 부품업체의 수가 증가였고, 현대차는 수도권에서, 기아차는 동남권에서 부품공급 거래가 확대되었다. 둘째, 완성차 공장을 중심으로 보면, 전속거래업체는 완성차 공장과 근접경향을 나타내고 있으며, 복수(네트워크)거래 업체들은 분산경향을 나타내고 있다. 또한 부품공급체계에 있어서 JIT(Just In Time, 적기공급)보다 더욱 세밀해진 JIS(Just In Sequence, 동기서열공급)체계를 확인할 수 있었다. 셋째, 사례연구를 통해 부품업체의 전략적 입지선정과정을 살펴볼 수 있었으며, 완성차 업체의 외부화 경향과는 반대로 규모의 경제를 위한 내부화 경향이 현저하게 나타났다.

• 대한기계학회논문집
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• 제12권2호
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• pp.389-396
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• 1988

본 연구에서는 위의 세가지 보간 알고리즘 가운데서 가장 속도의 균일성이 좋은 DDA방식을 채택하였다. 그리고 윤곽제어에서 가공경로는 대개 직선과 원호 구간의 조합으로 구성되어질 수 있기 때문에, 본 연구에서는 직선 및 원호보간만 고려하기로 한다.

• 산업공학
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• 제17권4호
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• pp.414-425
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• 2004

This study presents the decision of priority for optimal design guide to improve disassembly process. Disassembly process is divided into recognition, transfer and disassembly of assembly point and recognition, transfer and remove of grasp point. Significant influential factors are derived from analyzing the above process. And those factors are used for making the check list to evaluate the properties of parts in each process. Furthermore, the weight with considering disassembly process is also used to determine weight of each process. On the base of the above sequence, qualitative score of disassemblability of each process that is enabled to compare different disassembly processes can be acquired. Ultimately the score helps to decide the priority of design guide for disassembly process.

• 한국경영과학회:학술대회논문집
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• 대한산업공학회/한국경영과학회 1995년도 춘계공동학술대회논문집; 전남대학교; 28-29 Apr. 1995
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• pp.306-315
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• 1995

This paper presents a bin picking method using a slit beam laser in which a robot recognizes all of the unoccluded objects from the top of jumbled objects, and picks them up one by one. Once those unoccluded objects are removed, newly developed unoccluded objects underneath are recognized and the same process is continued until the bin gets empty. To recognize unoccluded objects, a new algorithm to link edges on slices which are generated by the orthogonally mounted laser on the xy table is proposed. The edges on slices are partitioned and classified using convex and concave function with a distance parameter. The edge types on the neighborhood slices are compared, then the hamming distances among identical kinds of edges are extracted as the features of fuzzy membership function. The sugeno fuzzy integration about features is used to determine linked edges. Finally, the pick-up sequence based on MaxMin theory is determined to cause minimal disturbance to the pile. This proposed method may provide a solution to the automation of part handling in manufacturing environments such as in punch press operation or part assembly.

• 산업경영시스템학회지
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• 제32권3호
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• pp.118-126
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• 2009

This research investigates the problem of minimizing setup costs in resequencing jobs having first-in, first-out(FIFO) constraints at conveyorized production or assembly systems. Sequence changing at conveyor junctions in these systems is limited due to FIFO restriction. We first define the general problem of resequencing jobs to workstations satisfying precedence relationships between jobs(Generalized Sequential Ordering Problem, GSOP). Then we limit our scope to FIFO precedence relationships which is the conveyor selection problem at a diverging junction(Diverging Sequential Ordering Problem, DSOP), modeling it as a 0-1 integer program. With the capacity constraint removed, we show that the problem can be modeled as an assignment problem. In addition, we proposed and evaluated the heuristic algorithm for the case where the capacity constraint cannot be removed. Finally, we discuss the case study which motivated this research and numerical results.

• 대한용접접합학회:학술대회논문집
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• 대한용접접합학회 2002년도 Proceedings of the International Welding/Joining Conference-Korea
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• pp.418-424
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• 2002

Korean Fusion Reactor(KSTAR) system consists of a vacuum vessel, in-vessel components, cryostat, thermal shield, super-conducting magnets and magnet supporting structures. These systems are in the final stage of engineering design with the involvement of industrial manufacturers. The overall configuration and the detailed dimensions of the KSTAR structure have been determined and the first stage of manufacturing is progressing now. In this study, the fabrication and assembly sequence were evaluated in viewpoint of high strengthening joints and very high accuracy. Especially for this purpose, the special cleaning process and welding process were proposed for high strengthening austenitic stainless steel which shall be used at cryogenic temperature. The draft procedure qualification data for welding process are presented with precise welding data including special narrow groove design. For the cooling line attachment on the surface of inside wall of magnet structure case, Induction brazing technology is introduced with some special jigging system and some consumables.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 1991년도 한국자동제어학술회의논문집(국내학술편); KOEX, Seoul; 22-24 Oct. 1991
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• pp.968-973
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• 1991

Seven axis modular type pneumatic manipulator is composed of electro-pneumatic automation system which contributes to factory automation by performing loading & unloading process successively which is simple routine work of dealing item of machine tool, catapult, assembly machine, welding machine and so on. In this study, we obtained soft and quick movement in a large space and good reliability motion of various function by combining several actuators which perform rotation movement as well as linear movement at the same time. Gripper which apply to rotary sensor transmitted a structure to demanded position. This development item of 5kgf load prevent stick-slip phenomena of stroke end by designing high cushion internal. We develope flexible manipulator which conforms to demand of user by applying multiple sequence program.