• 수산해양교육연구
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• 제28권2호
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• pp.450-455
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• 2016

Reinforcement for reduced daseulgi jawonryang protect natural resources, and form an artificial seed production and resource recovery in technology development and chipaebangryu doemeuroseo interested local governments, water 04, KEPCO daseulgi at promoting and stocking a lot of business, but are stocked in Format breed, stocking density, and a systematic way that does not occur in siljeong is discharged. Therefore, this study conducted by the appropriate discharge plan presented daseulgi smooth business execution and discharge of the business to be its primary purpose.

• 한국환경복원기술학회지
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• 제2권2호
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• pp.70-77
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• 1999

This study was carried out to suggest an effective method for the rehabilitation of Close-to-Nature River and artificial wetland. The results on the revegetation methods by seeding and sodding of Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides were summarized as follows. Seed germination of Phragmites japonica was 76.3% at $30^{\circ}C$, that of Miscanthus sacchariflorus was 68.7% at $20^{\circ}C$, that of Themeda triandra germinated 52.3% at $25^{\circ}C$, $30^{\circ}C$ constant temperature and that of Pennisetum alopecuroides germinated 86.7% at $30^{\circ}C/20^{\circ}C$ alternating temperature. Seed germination of Pennisetum alopecuroides exceeded 80% at $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$ constant temperature and $25^{\circ}C/15^{\circ}C$ alternating temperature. At 60 days after seeding, the ground coverage of Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides reached 81%, 81 %, 74%, and 86% respectively in the soil media of vermiculite and peatmoss(1 : 1 by volume). In the results of sod experiment, Phragmites japonica, Miscanthus sacchariflorus, Themeda triandra, and Pennisetum alopecuroides were formed sod completely in soil composition type of vermiculite and peatmoss(1 : 1, v/v). Thus this media seems to be best sad production media for rehabilitation works of Close-to-Nature River and man-made wetlands. Phragmites japonica and Miscanthus sacchariflorus were not significantly different in shoot height and the number of tillers by different planting distance for the first one year of experiment. Pennisetum alopecuroides shows high possibility to be used for Close-to-Nature River rehabilitation works by seeding.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2008년도 추계학술대회 논문집 Vol.21
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• pp.399-399
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• 2008

Synthesis and characterization of ZnO structure such as nanowires, nanorods, nanotube, nanowall, etc. have been studied to multifunctional application such as optical, nanoscale electronic and chemical devices because it has a room-temperature wide band gap of 3.37eV, large exiton binding energy(60meV) and various properties. Various synthesis methods including chemical vapor deposition (CVD), physical vapor deposition, electrochemical deposition, micro-emulsion, and hydrothermal approach have been reported to fabricate various kinds of ZnO nanostructures. But some of these synthesis methods are expensive and difficult of mass production. Wet chemical method has several advantage such as simple process, mass production, low temperature process, and low cost. In the present work, ZnO nanorods are deposited on ITO/glass substrate by simple wet chemical method. The process is perfomed by two steps. One-step is deposition of ZnO seeds and two-step is growth of ZnO nanorods on substrates. In order to form ZnO seeds on substrates, mixture solution of Zn acetate and Methanol was prepared.(one-step) Seed layers were deposited for control of morpholgy of ZnO seed layers by spin coating process because ZnO seeds is deposited uniformly by centrifugal force of spin coating. The seed-deposited samples were pre-annealed for 30min at $180^{\circ}C$ to enhance adhesion and crystallinnity of ZnO seed layer on substrate. Vertically well-aligned ZnO nanorods were grown by the "dipping-and-holding" process of the substrates into the mixture solution consisting of the mixture solution of DI water, Zinc nitrate and hexamethylenetetramine for 4 hours at $90^{\circ}C$.(two-step) It was found that density and morphology of ZnO nanorods were controlled by manipulation of ZnO seeds through rpm of spin coating. The morphology, crystallinity, optical properties of the grown ZnO nanostructures were carried out by field-emission scanning electron microscopy, high-resolution electron microscopy, photoluminescence, respectively. We are convinced that this method is complementing problems of main techniques of existing reports.

• 한국수산과학회지
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• 제43권6호
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• pp.687-692
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• 2010

The mass cultivation of Ecklonia cava Kjellman was studied as a potential biomass source for the extract industry in Korea. Experiments were conducted to investigate the optimal conditions for artificial seed production and mass cultivation of this species. Maximum growth and young thalli development in the nursery culture area occurred at 2 m depth, whereas maximum growth of thalli in the main culture area occurred at 1 m depth. Production of E. cava was between 2.6 and 3.6 kg wet wt. $m^{-1}$ after depth control and removal of fouling organism, etc. The relationship between optimal water depth for culture and underwater irradiance during the E. cava cultivation was calculated as: y = -0.718x + 8.042 ($r^2$=0.976). The growth rates achieved in this trial indicate that E. cava cultures could produce and supply sufficient biomass.

• 한국양식학회지
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• 제18권1호
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• pp.13-18
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• 2005

고부가가치 양식 대상종이고, 자원조성용으로 효과적인 돌돔의 안정된 종묘생산을 위한 연구의 일환으로서 수온, 먹이종류에 따른 성장과 생존 및 물 만들기를 실시하여 대량 종묘생산의 효과를 조사하였다. 자연해수 실험구($20.5^{\circ}C$)와 가온해수 실험구($25.3^{\circ}C$)조건에서 30일 동안 사육한 결과 가온구가 대조구 보다 2배정도 빠른 성장을 보여 유의차가 인정되었다. 생존율은 가온구에서 부화 후 30일까지 평균 $24.0\%$였고, 대조구에서는 $12.3\%$로서 상대적으로 낮았다. 먹이계열에 따른 자치어의 사육실험 결과 로티퍼와 배합사료를 공급한 실험구에서는 먹이효율이 낮았으나, 사육수조에 Nannochloropsis oculata 첨가하고, 로티퍼, 알테미아, 코페포다 및 배합사료를 혼합 공급한 실험구에서 성장 및 생존율에서 양호한 결과를 보여 유의한 차이가 인정되었다. 대량종묘생산 결과 일령 50일까지 생존율은 평균 $22.5\%$였으며, 치어의 평균 전장은 62.0$\pm$4.0mm로 성장하였다. 그리고 돌돔자어의 로티퍼 1회 섭식량은 부화 5일째평균 10.3개체, 10일째 16.5개체, 20일째 65.5개체로서 자어의 성장과 함께 섭식량은 급격히 증가하였다.

• 현장농수산연구지
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• 제23권2호
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• pp.15-24
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• 2021

왕우럭조개 양식산업화를 위해서는 왕우럭조개 서식지의 해양환경에 관한 기초자료 확보가 중요하다. 본 연구는 왕우럭조개 서식지 해양환경을 조사하여 인공종자생산을 위한 왕우럭조개 모패의 준비에 대한 기초자료를 확보하고자 하였다. 왕우럭조개 서식지의 수온은 겨울철에 가장 낮았으며, 여름철에 높게 나타났다. 염분은 31.2 ~ 33.9 psu로 조사시기에 따라 큰 차이가 없었다. pH는 7.69 ~ 8.70으로 겨울철에 높고, 여름철에 낮았다. 용존산소는 6.20 ~ 10.24 mg/L의 범위로 가을철이 봄철 및 겨울철에 비해 상대적으로 높게 나타났다. 식물 플랑크톤의 종 조성은 계절에 따라 약 30 ~ 40종 정도로 규조류가 대부분이었다. 계절별 식물플랑크톤 현존량은 23.5 ~ 61.3 cells/ml로 계절마다 차이를 보였다. 또한 우점종의 출현도 계절마다 차이를 보였다. 퇴적물 입도 조성은 모래 실트(sandy silt)이었다. 조류의 유향과 유속은 낙조시 남동방향으로 50 ~ 80 cm/s 나타났으며 창조시에는 북서방향으로 60 ~ 100 cm/s로 나타났다. 본 연구의 결과는 왕우럭조개의 서식지 해양환경에 대한 지식의 제공 및 잠입 서식하는 패류의 연구에 기초자료로 활용이 가능하다 사료된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.369-373
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• 2004

In order to elucidate the possibility of artificial production of p. ferulae by solid-state culture, the optimization of culture conditions was carried out. When $NH_4H_2PO_4$ and $CaCO_3$ were used in the cultures using test tube with 30 g of Populus sawdust at $25^{\circ}C{\pm}1$ in the dark, the favored mycelial growth was observed with $1\%$ of $NH_4H_2PO_4$ and the production of polysaccharide was 7.85 mg/100 mg of mycelium with $1\%$ of $CaCO_3$. The mixtures of $80\%$ of Populus Sawdust and $20\%$ of rice bran at $60\%$ of water content were determined to be optimal for the production of fruiting bodies in the sawdust culture. When three treatments containing various ratios of garlic powder were conducted, yields of fruiting bodies were drasti[ally higher than those of Synthetic mixture without garlic powder The highest yield (143 g/bag) was obtained with $7\%$ garlic powder. The yield of synthetic mixture containing $7\%$ of garlic powder was $83\%$ higher than that of Sawdust culture. The reason why garlic powder did support growth was not clear but it is possible that garlic powder might contain effective components for the formation of fruiting body. The optimal synthetic mixture composition consisted of cotton seed $77\%$, lime $6.4\%,\;K_2HPO_4\;0.2\%,\;KH_2PO_4\;0.2\%,\;CaHPO_4\;0.2\%$, corn flour $4\%$, wheat flour $5\%$, and garlic pow-der $7\%$.

• 한국수산과학회지
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• 제43권3호
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• pp.231-238
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• 2010

The Induction of regeneration and maturation in the free-living gametophytes of Ecklonia stolonifera Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol\;m^{-2}s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under $5{\sim}10^{\circ}C$, 10 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $20^{\circ}C$, 40 ${\mu}mol\;m^{-2}s^{-1}$, 10:14 h (L:D). The sexual maturation of female and male gametophytes was facilitated at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}s^{-1}$, 14:10 h (L:D). These results provide basic information for controlling the regeneration and maturation of the free-living gametophytes for artificial seed production of E. stolonifera.

• Ecology and Resilient Infrastructure
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• 제2권3호
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• pp.216-223
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• 2015

본 연구는 영주댐 건설사업 영향에 따른 멸종위기야생생물 1급 어류 흰수마자의 보전을 위하여 수몰예정지 내 흰수마자를 서식적합지로 이주시키고, 댐에 의한 내성천 상하류 단절 및 유전적 폐쇄에 대비하여 인공종묘생산 및 복원을 통해 내성천에 치어를 방류하여 그 결과를 관찰하였다. 영주댐 수몰예정지 내 흰수마자는 8회 이상 포획 시도에도 불구하고 서식이 확인되지 않아 이주가능지로 이주는 이루어지지 않았다. 내성천에서 포획한 친어 40개체를 통해 인공종묘생산한 치어 5,000개체는 친어의 유전 다양성을 물려받은 것으로 분석되었으며, 사전에 물리적, 생물학적 환경조건 분석을 통하여 자연 서식지와 유사한 최적방류지를 선정하여 인공종묘생산 치어를 방류하였다. 초기에는 방류지점을 중심으로 미소분산이 이루어졌으나, 시간흐름에 따라 확산이 진행되면서 재포획 개체수가 감소하였다. 포획된 방류 개체들은 내성천 자연생태환경에 적응하여 서식하고 있으며, 복부 팽만도가 높고 총배설공으로 배설물이 확인되는 점으로 보아 자연 먹이 섭식이 정상적으로 이루어지고 있는 것으로 나타났다. 따라서 인공종묘생산에 의한 영주댐 상하류 내성천 흰수마자 유전적 동질성 확보와 복원 개체군의 증강에 일차적 성공이 이루어지고 있는 것으로 파악되었으며 향후 장기적인 모니터링을 통한 지속적인 효과 분석이 필요할 것으로 전망되었다.

• 한국수산과학회지
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• 제44권2호
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• pp.155-161
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• 2011

Induction of regeneration and maturation of the free-living gametophytes of Undariopsis peterseniana (Kjellman) Miyabe et Okamura was studied at four temperatures (5, 10, 15, and $20^{\circ}C$), four levels of irradiance (5, 10, 20, and 40 ${\mu}mol$ $m^{-2}\;s^{-1}$) and three photoperiods (14:10, 12:12, and 10:14 h L:D). Female gametophyte fragments were maintained in active regeneration without reaching sexual maturity under conditions of $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 10:14 h (L:D), whereas the conditions for male gametophytes were slightly different at $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 14:10 h (L:D). The sexual maturation of female and male gametophytes took place under $15^{\circ}C$, 20 ${\mu}mol\;m^{-2}\;s^{-1}$, 14:10 h (L:D) and $10^{\circ}C$, 10 ${\mu}mol\;m^{-2}\;s^{-1}$, 10:14 h (L:D), respectively. These results provide basic information for controlling the regeneration and maturation of free-living gametophytes for artificial seed production of U. peterseniana.