• Journal of Microbiology and Biotechnology
• /
• 제16권3호
• /
• pp.443-449
• /
• 2006

Bacillus polyfermenticus SCD was studied for its increasing stability by encapsulation, using 2, 3, and 4% sodium alginate. In these cases, 3% alginate resulted in the maximum survival of B. polyfermenticus SCD in artificial gastric juice for 3 h. Effects of several biopolymers on the encapsulated B. polyfermenticus SCD by 3% sodium alginate were investigated. Encapsulation with 0.5% methylcellulose showed the highest survival rate for 3 h in artificial gastric juice. Therefore, the optimized encapsulation material was 3% alginate with 0.5% methylcellulose. Furthermore, the survival of encapsulated B. polyfermenticus SCD was shown to be 122%, when 1% bile salt was added. Freeze-dried encapsulation resulted in lower survival than with non-dried encapsulation. Therefore, encapsulation was the most effective when 3% sodium alginate was used with 0.5% methylcellulose, but without freeze-drying.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2019년도 추계학술대회
• /
• pp.154-154
• /
• 2019

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2019년도 춘계학술대회
• /
• pp.129-129
• /
• 2019

• Journal of Pharmaceutical Investigation
• /
• 제17권3호
• /
• pp.135-139
• /
• 1987

This study was attempted to investigate the dissolution rate and the bioavailability of commercially available sulfamethoxazole and trimethoprim (SMX-TM) tablets in rabbits. The dissolution test was conducted in artificial gastric juice by basket method with eight SMX-TM tablets which were chemically equivalent. According to the dissolution rate, SMX-TM tablets were divided into four groups, such as rapid, intermediate, slow and very slow groups for the bioavailability test in rabbits. The results were as follows: 1) The dissolution rate of brand A was most rapid but brand H was most slow in artificial gastric juice. 2) Area under the blood concentration curve was larger in the order of brand A > C > E > H in rabbits. 3) There was a little difference in pharmacokinetic parameters such as biological half life, absorption rate constant and $t_{max}$. 4) The relationship between the dissolution rate and relative bioavailability was significant in brand A, C, E and H. From the results of this experiment, the bioavailability of SMX-TM tablets in rabbits may be predicted from the results of dissolution rate studies.

• 미생물학회지
• /
• 제42권3호
• /
• pp.216-222
• /
• 2006

[ ${\beta}-Galactosidase$ ] (lactase)를 생산하는 유산균을 선별하고 그들이 생산하는 ${\beta}-galactosidase$의 특성을 조사하였다. X-gal이 포함된 MRS배지에서 푸른색을 나타내는 약 100여개의 콜로니를 김치로부터 분리하였다. 그들 중 ET-1과 LA-12 두 균주를 최종 선별하였으며, 16S rDNa 염기서열 분석을 통해 각 각 Lactobacillus fermentum과 L. acidophilus와 상동성이 높은 Lactobacillus 속으로 동정되었다. 선별된 두 균주는 높은 ${\beta}-galactosidase$ activeity와 우수한 생존력을 나타내었으며, 이들이 생산하는 ${\beta}-galactosidase$의 최적 활성조건을 조사한 결과, 반응온도 $55^{\circ}C$에서 가장 높은 활성을 나타내었으며, 반응 pH의 경우 ET-1은 pH 5.5에서 최고 활성을 나타내었고, LA-12는 pH7.0에서 가장 높은 활성을 보였다. 선별 유산균 ET-1과 LA-12는 위액과 담즙산액에 대해 우수한 내성을 나타내었다. 두 개의 선별 균주는 인공위액에 3시간 배양 후 초기 생균수와 비교하여 생균수 변화가 거의 없었고, 담즙산액으로 사용한 0.3% oxgall에서 24시간 반응 후에도 1 log cycle 정도 감소된 $10^8CFU/ml$의 생균수를 유지하였다. 이러한 결과를 바탕으로 유산균 ET-1과 LA-12는 유제품 산업에 이용 가능성이 크다고 판단된다.

• Fisheries and Aquatic Sciences
• /
• 제6권1호
• /
• pp.27-33
• /
• 2003

A lactic acid bacterium showing antimicrobial activity against fish pathogen was isolated from gastrointestinal tract of flounder for the purpose of use as an aquaculture probiotics. From the analysis of morphological and physiological characteristics, the isolated strain was named as Lactococcus sp. HM58. Antimicrobial substance (AMS) from Lactococcus sp. HM58 showed strong growth inhibitory activity against Streptococcus sp., which is a fish pathogenic bacterium. AMS was presumed a proteinaceous compound with stability in heat and wide pH range from 2 to 10. It was started to produce in exponential growth phase and was not produced any more in stationary phase. It showed comparatively broad antimicrobial spectrum against most of gram positive bacteria used for this study. About $84\%$ of Lactococcus sp. HM58 was able to survive in the artificial gastric juice though it was low to the extent in the artificial bile juice. In the sensitivity test for various antibiotics, this strain was highly sensitive for doxycycline, erythromycin, amoxicillin clavu1anic acid and ampicillin.

• 한국식품과학회지
• /
• 제17권3호
• /
• pp.192-196
• /
• 1985

국내 액상발효유 7개 회사 제품을 수거하여 미생물학적인 특성을 조사하였다. 7개 회사 제품(A, B, C, D, E, F 및 G)에서 분리한 8종의 유산균(a, b, b', c, d, e, f 및 g)을 동정해 본 결과 A회사 제품의 a균과 E회사 제품의 e균은 L. casei로 B회사 제품의 b균은 L. acidophilus로, B회사 제품의 b'균, C 회사 제품의 c균 및 G회사 제품의 g균은 L. jugurti로, F 회사 제품의 f균은 L. helveticus로, D회사 제품의 d균은 L. bulgaricus로 각각 동정되었다. L. helveticus로 동정된 f균과 L. jugurti로 동정된 c균은 인공위액에 대한 내성은 높았으나 담즙산에 대한 내성은 높지 못했다. L. acidophilus로 동정된 b균과 L. casei로 동정된 a균 및 e균은 인공위액과 담즙산에 대한 내성이 모두 높았고, L. bulgaricus로 동정된 d균과 L. jugurti 동정된 b'균 및 g균은 인공위액과 담즙산에 대한 내성이 모두 낮았다.

• Journal of Pharmaceutical Investigation
• /
• 제17권2호
• /
• pp.79-88
• /
• 1987

In order to reduce gastric irritation of iron preparations, one prodrug, glycine complex of ferrous fumarate, was synthesized, identified, examined for physico-chemical properties and compared with ferrous fumarate, ferrous sulfate and ferroglycine sulfate. That novel ferroglycine fumarate (FGF) resulted in higher dissolution rate in water, artificial gastric and intestinal juice. The absorption rate constants $(K_a)$ in rat of FGF was greater and ulcerogenic dose on stomach was increased remarkably than those of iron parent materials and ferroglycine sulfate.

• 약학회지
• /
• 제30권3호
• /
• pp.128-138
• /
• 1986

Three newly synthesized alkanol esters of d-2-(6-methoxy-2-naphthyl) propionic acid, NAPROXEN were examined for physicochemical properties and biopharmaceutical characteristics. These esters were very stable in solid state, but more than 90% of these esters were hydrolysed to the parent, naproxen in rabbit's liver hornogenates. They showed higher dissolution rate in the artificial gastric and intestinal juice, and significantly greater partition coefficient in n-octanol, when compared with naproxen. The absorption rate constants of these esters were increased, while the elimination rate constants were decreased, comparing with naproxen. The ulcerogenic doses on gastric and intestinal mucosa were increased remarkably, and the antiinflammatory dose against carrageenininduced edema on rat hind paw was decreased markedly in these esters, and thus the safety indexes of these esters were higher than that of naproxen.

• Journal of Pharmaceutical Investigation
• /
• 제30권1호
• /
• pp.47-50
• /
• 2000

A new technique has been developed for the preparation of Lactobacillus microcapsules to enhance the stability against high temperature, humidity, gastric acid and bile acid. Employing fluidized bed coating, primary sub-coating was processed in non-organic solvent system, so that Lactobacillus did not directly contact with organic solvent. Secondary enteric-coating was processed in organic solvent with low temperature $(below\;33^{\circ}C)$ technique, which minimized the heat labilability of Lactobacillus. Survival rate of Lactobacillus within microcapsule was not less than 95% and acid tolerance was above 30% in the artificial gastric acid. Further more it was dissolved in the artificial intestine juice within 2-3 hr. Average size of Lactobacillus microcapsules was $450\;{\mu}m$(25-50 mesh) and its viability was above 90% in the direct tableting.