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Characterization and Selection of Lactic Acid Bacteria Producing ${\beta}-Galactosidase$  

Lee, Young-Ki (Environmental Biotechnology Research Center, KRIBB)
Choi, Susanna (Department of Biotechnology, Catholic University of Korea)
Park, Young-Il (Department of Biotechnology, Catholic University of Korea)
Park, Chan-Sun (Environmental Biotechnology Research Center, KRIBB)
Yoon, Byung-Dae (Environmental Biotechnology Research Center, KRIBB)
Hwang, Yun-Sik (Anichem Company Ltd., KRIBB BVC)
Kim, Hee-Sik (Environmental Biotechnology Research Center, KRIBB)
Publication Information
Korean Journal of Microbiology / v.42, no.3, 2006 , pp. 216-222 More about this Journal
Abstract
This study was carried out to select the lactic acid bacteria producing ${\beta}-galactosidase$ (lactase) and investigate the properties of the ${\beta}-galactosidase$. About 100 strains of lactic acid bacteria showing blue colony on the MRS agar medium containing X-gal were isolated from several kinds of Kimchi. Among them, 2 strains were selected as potential ${\beta}-galactosidase$ producers. The selected strains, ET-1 and LA-12, were identified as Lactobacillus fermentum and L. acidophilus, respectively by the analysis of 16S rDNA sequences. They showed relatively high ${\beta}-galactosidase$ activity and cellular viability. Their ${\beta}-galactosidase$ showed the highest activity at $55^{\circ}C$. And the optimum pHs of the enzymes produced by ET-1 and LA-12 were pH 5.5 and pH 7.0, respectively. They were also highly resistant to artificial gastric juice and bile. Two selected strains showed little change of viable cell number for 3 hr incubation in artificial gastric juice, and maintained the viable cell number at $10^8CFU/ml$ for 24 hr in 0.3% oxgall after incubation for 2 hours in artificial gastric juice. Based on these results, ET-1 and LA-12 are expected to be applied in dairy industry.
Keywords
${\beta}-Galactosidase$; Lactobacillus;
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