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Encapsulation of Bacillus polyfermenticus SCD with Alginate-Methylcellulose and Evaluation of Survival in Artificial Conditions of Large Intestine  

Kim Cheon-Jei (Division of Animal Life Science, Konkuk University)
Jun Song-Ae (Division of Animal Life Science, Konkuk University)
Lee Na-Kyoung (Division of Animal Life Science, Konkuk University)
Kim Kee-Tae (Bio/Molecular Informatics Center, Konkuk University)
Lee Si-Kyung (Department of Applied Biology and Chemistry, Konkuk University)
Kim Chang-Han (Division of Animal Life Science, Konkuk University)
Paik Hyun-Dong (Division of Animal Life Science, Konkuk University)
Publication Information
Journal of Microbiology and Biotechnology / v.16, no.3, 2006 , pp. 443-449 More about this Journal
Abstract
Bacillus polyfermenticus SCD was studied for its increasing stability by encapsulation, using 2, 3, and 4% sodium alginate. In these cases, 3% alginate resulted in the maximum survival of B. polyfermenticus SCD in artificial gastric juice for 3 h. Effects of several biopolymers on the encapsulated B. polyfermenticus SCD by 3% sodium alginate were investigated. Encapsulation with 0.5% methylcellulose showed the highest survival rate for 3 h in artificial gastric juice. Therefore, the optimized encapsulation material was 3% alginate with 0.5% methylcellulose. Furthermore, the survival of encapsulated B. polyfermenticus SCD was shown to be 122%, when 1% bile salt was added. Freeze-dried encapsulation resulted in lower survival than with non-dried encapsulation. Therefore, encapsulation was the most effective when 3% sodium alginate was used with 0.5% methylcellulose, but without freeze-drying.
Keywords
Probiotics; encapsulation; alginate; Bacillus polyfermenticus;
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Times Cited By KSCI : 4  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
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