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Isolation and characterization of BrMDR1 a novel MDR-type ATP-binding cassette (ABC) transporter in Brassica rapa L.

  • Lee, Sun-Yong;Jung, Yu-Jin;Kang, Kwon-Kyoo
    • Korean Journal of Plant Resources
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    • v.22 no.3
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    • pp.273-280
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    • 2009
  • A cDNA clone encoding a MDR-like ABC transporter protein was isolated from Brassica rapa seedlings, through rapid amplification of cDNA ends (RACE). This gene (named as Brmdr 1; GenBank accession no.: DQ296184 ) had a total length of 4222 bp with an open reading frame of 3900 bp, and encoded a predicted polypeptide of 1300 amino acids with a molecular weight of 143.1 kDa. The BrMDR1 protein shared 71.0, 62.5, 60.0 and 58.2% identity with other MDR proteins isolated from Arabidopsis thaliana (AAN28720), Coptis japonica (CjMDR), Gossypium hirsutum (GhMDR) and Triticum aestivum (TaMDR) at amino acid level, respectively. Southern blot analysis showed that Brmdr1 was a low-copy gene. Expression pattern analysis revealed that Brmdr1 constitutively expressed in the root, stem petals and stamens, but with lower expression in leaves and open flowers. The domains analysis showed that BrMDR1 protein possessed two transmembrane domains (TMDs) and two nucleotide binding domains (NBDs) arranging in "TMD1-NBD1-TMD2-NBD2" direction, which is consistent with other MDR transporters. Within NBDs three characteristic motifs common to all ABC transporters, "Walker A", "Walker B" and C motif, were found. These results indicate that BrMDR1 is a MDR-like ABC transporter protein that may be involved in the transport and accumulation of secondary metabolites.

Factor Analysis for Improvement of Convenience and Safety in the Interior Design of Subway Trains (지하철 차량 실내디자인의 편의성과 안전성 증진을 위한 요소 분석)

  • Jin, Mi-Ja;Han, Suk-Woo
    • Proceedings of the KSR Conference
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    • 2007.05a
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    • pp.643-652
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    • 2007
  • This study identifies visible/invisible factors and analyzes facilities, structure, and visible perceptive contents in the aspects of operation and efficiency with a focus on the interfaces between interior design of the subway and users. More specifically, it diagnoses the requirements and empirically presents improvements. In addition, this study aims to diagnose the interfaces between trains and users by reinterpreting and evaluating factors from the perspectives of users, and to elicit the universal interface and an objective form of conceptual, structural, and formative relations in regard to the conditions required to the design. As the interior design in the subway trains is for the public reflecting the value, consciousness, and behaviors of users, it should be approached through the application of the concept of universal design that considers the potential needs and psychological satisfaction of users. In arranging the indoor of the vehicles, the conditions for the facilities should be specified through the interdisciplinary approach from professional fields including ergonomics and psychology, the path of flow should be induced through the use of space and the analysis of passengers' behaviors, an integrated planning should be re-established, and continuous evaluations of basic guidelines, manuals, and requirements should be made.

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The Gap of the Practice and the Needs for Improving Underachievers' Study Habits (학습부진학생 대상의 학습습관 지도 요구와 실행 정도 간 차이 분석)

  • Kang, Kyung-Sook;Hahm, Seung-Yeon;Noh, Jung-Eun
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.4
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    • pp.877-893
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    • 2014
  • The purpose of this research is to study the implementation of instructional practices and the needs for improving study habits of underachievers, as a preliminary research to develop improving program for underachievers' study habits. Firstly, past researches on the subject were analyzed to find out the concept and scope of study habits, and factors related to academic achievement. After arranging and integrating these factors, we have extracted the constructs and factors of improvement program for students' study habits. Secondly, the instructional practices in the field and needs for improvement program for students' study habits were analyzed through a survey. The online survey composed of structured questionnaire was conducted among teachers consisting of regular classroom teachers, special class teachers, school counselors, etc. The results were reflected in the contents and configuration of the program. The contents of survey was the degree of practice and needs of improvement program for students' study habits in schools, and the degree of practice and needs of standardized tests. According to the survey results, the efforts for improving the underachieved students' study habits were low level. This study focused on study habits showing high demands in the development of program and manual.

User-created multi-view video generation with portable camera in mobile environment (모바일 환경의 이동형 카메라를 이용한 사용자 저작 다시점 동영상의 제안)

  • Sung, Bo Kyung;Park, Jun Hyoung;Yeo, Ji Hye;Ko, Il Ju
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.8 no.1
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    • pp.157-170
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    • 2012
  • Recently, user-created video shows high increasing in production and consumption. Among these, videos records an identical subject in limited space with multi-view are coming out. Occurring main reason of this kind of video is popularization of portable camera and mobile web environment. Multi-view has studied in visually representation technique fields for point of view. Definition of multi-view has been expanded and applied to various contents authoring lately. To make user-created videos into multi-view contents can be a kind of suggestion as a user experience for new form of video consumption. In this paper, we show the possibility to make user-created videos into multi-view video content through analyzing multi-view video contents even there exist attribute differentiations. To understanding definition and attribution of multi-view classified and analyzed existing multi-view contents. To solve time axis arranging problem occurred in multi-view processing proposed audio matching method. Audio matching method organize feature extracting and comparing. To extract features is proposed MFCC that is most universally used. Comparing is proposed n by n. We proposed multi-view video contents that can consume arranged user-created video by user selection.

Recognition of road information using magnetic polarity for intelligent vehicles (자계 극배치를 이용한 지능형 차량용 도로 정보의 인식)

  • Kim, Young-Min;Lim, Young-Cheol;Kim, Tae-Gon;Kim, Eui-Sun
    • Journal of Sensor Science and Technology
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    • v.14 no.6
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    • pp.409-414
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    • 2005
  • For an intelligent vehicle driving which uses magnetic markers and magnetic sensors, we can get every kind of road information while moving the vehicle if we use the code that is encoded with N, S pole direction of markers. If we make it an only aim to move the vehicle, it becomes easy to control the vehicle the more we put markers close. By the way, to recognize the direction of a marker pole it is much better that the markers have no effect each other. To get road informations and move the vehicle autonomously we propose the methods of arranging magnetic sensors and algorithm of recognizing the position of the vehicle with those sensors. We verified the effectiveness of the methods with computer simulation.

The Design and Fabrication of SRAM Modules Surface Mounted on Multilayer Borads (다층 기판 위에 표면실장된 SRAM 모듈 설계 제작)

  • Kim, Chang-Yeon;Jee, Yong
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.32A no.3
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    • pp.89-99
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    • 1995
  • In this paper, we ecamined the effect that MCM-L technique influencess on the design and fabrication of multichip memory modules in increasing the packing desity of memory capacity and maximizing its electrical characteristics. For that purpose, we examined the effective methods of reducing the area of module layout and the wiring length with the variation of chip allocation and the number of wiring layers. We fabricated a 256K${\times}$8bit SRAM module with eight 32K${\times}$8bit SRAM chips. The routing experiment showed that we could optimize the area of module layout and wiring length by placing chips in a row, arranging module I/O pads parallel to chip I/O pads, and equalizing the number of terminal sides of module I/O's to that of chip I/O's. The routing was optimized when we used three wire layers in case of one sided chip mounting or five wire layers in case of double sided chip mounting. The fabricated modules showed 18.9 cm/cm$^{2}$ in wiring density, 65 % in substrate occupancy efficiency, and module substrate and functionally tested to find out the module working perfectly.

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Analysis on the Geo-reinforced Slope Using Upper Bound Theory (상계해석을 이용한 보강토 사면의 해석)

  • Choi Sang-Ho;Kim Jong-Min;Yu Nam-Jae
    • Journal of the Korean Geotechnical Society
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    • v.20 no.7
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    • pp.207-215
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    • 2004
  • In this study, the upper bound theory is applied to a reinforced slope to develop an limit state analysis method. As processing of this upper bound theory in formulating finite element, the basic idea of numerical method can be obtained from a macroscopic point of view with an anisotropic homogeneous material. The reinforced soil strength reliability depends on properties of reinforcements which consist of the interaction of interfaces between back fill and reinforcements. Both soil's mechanical property and overall behaviour of reinforced soil can be controlled via arranging geometry and relative proportions of reinforced soil. Therefore, the upper bound theory can not only predict the particular limit state action of reinforced soil slope but also is efficiently able to estimate the local plastic failure.

A Study on the Characteristics of Jeju Recreational Pension (제주도 휴양펜션시설의 건축실태에 관한 연구)

  • Jeong, Byeol-Ah;Kim, Bong-Ae
    • Proceeding of Spring/Autumn Annual Conference of KHA
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    • 2004.11a
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    • pp.135-140
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    • 2004
  • The purpose of this research is to investigate the characteristics of Jeju recreational pension. This research is carried out by on site inspection and the survey on management of the recreation facilities. A target of research is sixteen facilities of recreation pension in Jeju-do. Recreational pension was created by Jeju Free International City of special law in 2000. Jeju Recreational pension has characteristics as follows : The land area of that is $1300m^{2}{\sim}6300m^{2}$, the number of guest rooms are 10, and the area of guest rooms are $29m^{2}{\sim}100m^{2}$. Especially most of them are in Namjeju-gun. Also, they mostly have R.C. structure, and materials of exterior are artificiality stone. The color of an outer walls are mostly white and light-beige and that of the roofs are mainly dark-brown. On plot plan, it has 4type of prototype; type of outbuilding, arranging in a row, corridor, hall and composition of three types with public area. The outside facilities are equipped by handy golf courses and outdoor barbecue cooking sites. Unfortunately, most of pensions don't reflect the characteristics of architecture factors in Jeju.

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A Study on the Characteristics of House Arrangement of Unified Silla Period - Focused on the Capital Remains of Silla in Gyeongju - (통일신라시대 주택의 배치특성 - 경주 신라왕경 발굴유구를 중심으로 -)

  • Han, Ji-Man;Lee, Jeong-Mee
    • Journal of the Korean housing association
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    • v.27 no.3
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    • pp.23-30
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    • 2016
  • Among the unearthed House ruins in Gyeongju of the capital of Silla period, the ruins to see the overall appearance of building arrangement is the ruins of Silla capital S1E1 area, Inwangdong 412, and the Jaemaejeong. In this study, the characteristics of urban house arrangement of the Unified Silla period was analyzed that, through a review of the arrangement relationship between the gate and the individual buildings found in these ruins. The urban house of the Unified Silla period was surrounded with wall, and the way to distinguish between functional areas within it are shown differently, depending on the size of the house. In other words, the small house was divided each area by installation of inner fence, and the large house was by arranging attached buildings. Thai is, the central area is not divided by inner fence is the Characteristics that is different from the small house. And in all houses, a large courtyard is located in the front of main building. Conatruction of the courtyard determines the location and direction of the main building. And the each area has external space of courtyard in the center.

Study on the Characteristic of Partiality in Korean Traditional Residential Architecture in view of the Phenomenology (현상학 측면에서 본 한국 전통주거건축의 부분성에 관한 연구)

  • Yook, Ok-Soo
    • Journal of the Korean housing association
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    • v.27 no.5
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    • pp.73-81
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    • 2016
  • Focused on developing form of Western architecture, invisible elements covering actual human life has been dealt with separately and the time was turned away and disparaged. But now the phenomenology, arranging such a meeting of space and time, gives opportunity to look at the actual life in architecture. Recently, Guille Deleuze and Merleau-Ponty tried to recover the sense of touch segregated in skin from body keeping eyes for distance. And the activity can be happened by being connected to the body rather than to eye in the space. From the phase of recognition where the human body tries to identify the object in the space considering a time, it will be changed for the subject to the phase of space-time structure. If the tactility is to erase the distance different from the visuality in dichotomy, it will be occurred to having a tension and makes new relationship to work trying to move the subjective point of view in object. Like this evidence in analysis of architecture, it can be found these in the Korean traditional architecture rather than Western architecture in terms of emphasizing the time and space. And this study will be intensive to the side of the Phenomenology how it is being connected to the movement in the space and time for the subject and object.