• 생약학회지
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• 제38권4호
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• pp.349-353
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• 2007

Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase-I(MMP-1) activity. This study was carried out to find out skin wrinkle reducing components in Torilis Fructus. Torilis Fructus were extracted with 70% ethanol and the ethanol extracts were systematically fractionated with n-hexane, ethylacetate, n-butanol and distilled water. Among them, antiwrinkle component from n-hexane fraction was purified by several column chromatographies and HPLC, which identified as torilin by $^1H-NMR,\;^{13}C-NMR$ and ESI-MS. To determine cell viability, collagen biosynthesis and MMP-1 activity, human dermal fibroblast was treated with 1-5 ppm concentrations of Torilis Fructus extract fraction and torilin. Cell viability was showed 84-102% at all group treated with 1-5 ppm. Collagen synthesis was increased in all group, especially torilin-treated group was highest amount. Active forms of MMP-1 were decreased in all group. From these results, we consider that Torilis Fructus have several antiwrinkle components and torilin may be one of the effective components.

• Korean Journal of Acupuncture
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• 제31권4호
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• pp.188-194
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• 2014

Objectives : In this study, we investigated the effect of ethanol extract from Rosa rugosa Thunb. flowers(ERF) on the activities of antioxidant, antiwrinkle and whitening. Methods : We measured antioxidant efficacy of ERF by using 1,1-diphenyl-2-picrylhydrazyl(DPPH) assay. Also we confirmed the inhibitory effect of ERF on collagenase activity and melanin synthesis by using collagenase assay kit and dihydroxiphenylalanine staining, respectively. To evaluate the anti-inflammatory effects of ERF, we examined the inflammatory mediator IL-6. Results : ERF showed highly efficacy in DPPH radical scavenging activity. ERF dose-dependently suppressed collagenase activity. Ultraviolet-induced production of IL-6 decreased by ERF treatment. In B16F10 cell, ERF significantly reduced tyrosinase activity and melanin synthesis. Conclusions : From the above results, it was indicated that ERF could be utilized as anti-aging and whitening cosmetic ingredients.

• 한국해양바이오학회지
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• 제14권1호
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• pp.9-24
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• 2022

Low-molecular weight peptides derived from fish collagen exhibit several bioactivities, including antioxidant, antiwrinkle, antimicrobial, antidiabetic, and antihypertension effects. These peptides are also involved in triglyceride suppression and memory improvement. This study aimed to investigate the optimal processing condition for preparing low-molecular weight peptides from flounder skin, and the properties of the hydrolysate. The optimal processing conditions for peptic hydrolysis were as follows: a ratio of pepsin to dried skin powder of 2% (w/w), pH of 2.0, and a temperature of 50℃. Peptic hydrolysate contains several low-molecular weight peptides below 300 Da. Gly-Pro-Hyp(GPHyp) peptide, a process control index, was detected only in peptic hydrolysate on matrix-assisted laser desorption/ionization-time-of-flight(MALDI-TOF) spectrum. 2,2'-azinobis-(3-3-ethylbenzothiazolline-6- sulfonic acid(ABTS) radical scavenging activity of the peptic hydrolysate was comparable to that of 1 mM ascorbic acid, which was used as a positive control at pH 5.5, whereas collagenase inhibition was five times higher with the peptic hydrolysate than with 1 mM ascorbic acid at pH 7.5. However, the tyrosinase inhibition ability of the peptic hydrolysate was lower than that of arbutin, which was used as a positive control. The antibacterial effect of the peptic hydrolysate against Propionibacterium acne was not observed. These results suggest that the peptic hydrolysate derived from a flounder skin is a promising antiwrinkle agent that can be used in various food and cosmetic products to prevent wrinkles caused by ultraviolet radiations.

• 대한화장품학회지
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• 제38권2호
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• pp.155-162
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• 2012

숙성된 인삼은 인간의 건강을 회복시키고 향상시키는 강장제로서 알려져 있으며, 백삼보다 더 많은 약리적인 활성을 가진다고 알려져 왔다. 전이 가능한 종양, 림프구 증식과 쥐간의 지질과산화에 대한 숙성된 인삼의 효과도 연구되었다. 본 연구는 3가지 인삼 추출물의 항산화와 항노화 효과를 평가하기 위해서 실험하게 되었다. 시장에서 이미 만들어진 홍삼엑기스를 사용하였고, 그 외 수삼, 건삼을 구매하여 홍삼제조기로 만들어진 추출물로 실험에 사용하였다. 숙성된 인삼의 3가지 추출물들은 superoxide radical 소거 활성, hydroxyl radical 소거 활성, 리놀산 자동산화 억제, 섬유아세포에서 collagenase 억제와 collagen 합성의 효과를 연구했다. 숙성시켜 만든 수삼은 100 ug/mL의 농도에서 가장 높은 superoxide radical 소거 활성뿐만 아니라 vitamin C보다 더 높은 hydroxyl radical 소거 활성을 나타냈다. 또한 리놀산 자동산화 억제, collagen 합성과 collagenase 억제에서 가장 높은 활성을 보였다.

• 생명과학회지
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• 제23권10호
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• pp.1239-1245
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• 2013

청미래덩굴 추출물의 주름개선 효과를 확인하기 위해 elastase 저해활성을 측정한 결과 청미래덩굴 에탄올 추출물과 열수 추출물이 $1,000{\mu}g/ml$에서 각각 41.1%, 16.3%의 활성을 나타내었으며, collagenase 저해활성 측정결과 청미래덩굴 에탄올 추출물과 열수 추출물이 $1,000{\mu}g/ml$에서 각각 96.6%, 60.0%의 활성을 나타내었다. 청미래덩굴의 에탄올 추출물의 세포생존율을 확인하기 위하여 섬유아세포(CCD-986sk)로 MTT assay에 의해 확인한 결과 $50{\mu}g/ml$ 농도에서 71.7%의 세포 생존율을 확인할 수 있었다. 섬유아 세포에서 청미래덩굴 에탄올 추출물의 pro-collagen type-1 생합성량을 확인한 결과 $50{\mu}g/ml$의 농도에서 139% 이상의 우수한 효과를 나타내었으며, MMP-1 저해활성을 측정한 결과 $50{\mu}g/ml$의 각각의 농도에서 74.9%의 활성을 나타내었다. 청미래덩굴 에탄올 추출물을 처리한 섬유아 세포에서 MMP family 중 MMP-1의 단백질 발현 및 mRNA 발현 억제 효과를 확인한 결과 MMP-1 단백질 발현과 mRNA 발현 모두 $50{\mu}g/ml$에서 각각 35%, 45%의 저해율을 나타내었다. 이러한 결과로 미루어 보아 청미래덩굴 에탄올 추출물이 주름개선용 화장품 소재로 응용이 가능할 것으로 판단된다.

• 한국약용작물학회지
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• 제26권2호
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• pp.119-126
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• 2018

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity ($RC_{50}=10.27{\mu}g/m{\ell}$), and the TPC and TFC also were highest in MIX ($225.7mg{\cdot}GAE/g$, and $25.18mg{\cdot}QE/g$, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

• 한국식품영양과학회지
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• 제37권9호
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• pp.1136-1141
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• 2008

무모쥐의 등에 애엽 추출물과 아스코르브산 함유 스킨로션을 도포하고 자외선을 조사하여 애엽 추출물과 아스코르브산의 주름 개선 효과를 알아보았다. 애엽 추출물 도포군의 피부조직 두께는 대조군에 비해 $12.5{\sim}21.4%$의 유의적인 감소 효과를 보였다. 표면 거칠기 측정기에 의한 피부 주름의 형성 정도를 측정하는 지표인 Ra값은 ME-1.0, ME-2.0과 ME-5.0 도포군에서 $23.7{\sim}31.1%$로 유의적으로 감소하였고 (p<0.01), Rq치는 ME-1.0, ME-2.0와 ME-5.0에서 $11.2{\sim}21.2%$, Rz의 측정치에서는 ME-1.0(19.8%), ME-2.0(22.1%), ME-5.0(24.5%) 그룹에서 대조군에 비해 크게 감소하였다. Rt치는 ME-1.0, ME-2.0와 ME-5.0 그룹에서 농도 증가에 따라 14.2%에서 22.7%의 유의적인 감소효과가 있었다. 아스코르브산 도포그룹은 애엽과 비슷한 감소효과를 보여주었지만, 애엽의 주름 개선효과에는 미치지 못하였다. MMP-1 활성은 애엽 메탄올 추출물 그룹에서 $19.3%{\sim}22.6%$까지 감소하였고, 콜라겐의 생성은 ME-2.0과 ME-5.0그룹에서 10% 정도의 유의적인 증가 효과가 있었다. 이처럼 애엽 메탄올 추출물은 피부의 광손상에 대한 피부 보호 및 피부의 주름 개선효과가 탁월하고, 또한 강력한 항산화제인 아스코르브산보다 그 효과가 우수하여 피부노화 현상과 주름 개선을 위한 기능성 화장품 소재로 이용가치가 있을 것으로 여겨진다.

• 한국약용작물학회지
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• 제19권4호
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• pp.264-270
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• 2011

In this study, we investigated the cosmetic application of Acer mono sap through an ultra-high pressure process. Exposing Acer mono sap to a ultra-high pressure process resulted in 90.1% cell viability of human normal fibroblast cells (CCD-986sk) when added at the highest concentration. Acer mono sap also showed the hightest free radical scavenging activity after the ultra high pressure process. The melanogenesis inhibition rate in cloned M-3 cells was 59.0%. Tyrosinase was inhibited at a rate of 87.2% by adding 100% HPAMS. Anti-wrinkle activity was 78.1%. Acer mono sap showed enhanced storage following the ultra high pressure process. These results indicate that Acer mono sap may be a source for functional cosmetic agents capable of improving antioxidant, whitening, and antiwrinkling effects.

• Korean Chemical Engineering Research
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• 제62권2호
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• pp.142-146
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• 2024

전세계적으로 천연화장품에 대한 관심과 수요가 크게 늘어나고 있다. 따라서 기존 화학합성 기능성화장품소재를 대체하기 위한 노력의 일환으로서 천연물에서 기능성화장품소재를 발굴하고자 하였다. 모자반은 우리나라의 해안 전지역에서 쉽게 볼 수 있는 갈조류의 일종으로서 식용으로도 이용되는 만큼 안전성이 확보되어있는 해조류이다. 본 연구에서는 큰잎 모자반(Sargassum coreanum). 짝잎 모자반(Sargassum hemiphyllum), 쌍발이 모자반(Sargassum patens)의 3가지 종류의 모자반으로부터 열수를 이용하여 유효성분을 추출하였으며, 항산화효과, 미백효과, 주름개선효과, 자외선흡수효과 및 항염증효과를 측정하여 기능성 화장품 소재로서의 응용 가능성을 살펴보고자 하였다. 모자반추출물은 세포독성시험결과 안전성이 매우 우수하였고, DPPH radical scavenging activity를 이용한 항산화능 측정결과 우수한 항산화능을 보여주었으며 tyrosinase inhibition을 이용한 미백효과시험에서는 큰잎 모자반(Sargassum coreanum)의 미백효과가 상당히 우수하였다. 그러나 주름개선효과, 자외선흡수효과와 항염증효과는 비교적 낮았다. 결론적으로 큰잎 모자반(Sargassum coreanum)은 세포독성이 적었고, 항산화효과와 미백효과가 우수하여 미백 기능성화장품소재로서 응용가능성이 높음을 알 수 있었다.

• 한국약용작물학회지
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• 제19권2호
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• pp.126-135
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• 2011

Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.