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Effect of Torilis Fructus on Procollagen Biosynthesis and Activity of Matrix Metalloproteinase-I(MMP-1) in Human Dermal Fibroblast  

Koo, Bon-Suk (Health Food Research Group, KT&G Central Research Institute)
Hwang, Eui-Il (Health Food Research Group, KT&G Central Research Institute)
So, Seung-Ho (Health Food Research Group, KT&G Central Research Institute)
Lee, Seong-Kye (Health Food Research Group, KT&G Central Research Institute)
Han, Gyeong-Ho (Health Food Research Group, KT&G Central Research Institute)
Kim, Na-Mi (Health Food Research Group, KT&G Central Research Institute)
Publication Information
Korean Journal of Pharmacognosy / v.38, no.4, 2007 , pp. 349-353 More about this Journal
Abstract
Skin wrinkle formations are associated with collagen synthesis and matrix metalloproteinase-I(MMP-1) activity. This study was carried out to find out skin wrinkle reducing components in Torilis Fructus. Torilis Fructus were extracted with 70% ethanol and the ethanol extracts were systematically fractionated with n-hexane, ethylacetate, n-butanol and distilled water. Among them, antiwrinkle component from n-hexane fraction was purified by several column chromatographies and HPLC, which identified as torilin by $^1H-NMR,\;^{13}C-NMR$ and ESI-MS. To determine cell viability, collagen biosynthesis and MMP-1 activity, human dermal fibroblast was treated with 1-5 ppm concentrations of Torilis Fructus extract fraction and torilin. Cell viability was showed 84-102% at all group treated with 1-5 ppm. Collagen synthesis was increased in all group, especially torilin-treated group was highest amount. Active forms of MMP-1 were decreased in all group. From these results, we consider that Torilis Fructus have several antiwrinkle components and torilin may be one of the effective components.
Keywords
human dermal fibroblast; collagen biosynthesis; matrix metalloproteinase-I; torilin; Torilis Fructus;
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