Browse > Article
http://dx.doi.org/10.5352/JLS.2013.23.10.1239

Inhibitory Efficacy of Smilax china L. on Pro-collagen Type-1 Activity and MMP-1 Gene Expression in Fibroblasts (CCD-986sk)  

Lee, Soo-Yeon (Department of Herbal Cosmetic Science, Hoseo University)
Lee, Jin-Young (Department of Herbal Cosmetic Science, Hoseo University)
Publication Information
Journal of Life Science / v.23, no.10, 2013 , pp. 1239-1245 More about this Journal
Abstract
The purpose of this study was to research the biological activity of ethanol extract from Smilax china L. which is a vine shrub belonging to the lily family. For antiwrinkle effects, elastase inhibition effect of ethanol and water extracts from S. china L. showed 41.1% and 16.3% at $1,000{\mu}g/ml$ concentration. The collagenase inhibition effect of ethanol and water extracts from S. china L. showed more than 96.6% and 60.0% at $1,000{\mu}g/ml$ concentration. As a result of having fibroblast measured cell viability on fibroblast cell of ethanol extract from S. china L., it showed 71.7% with cell viability at $100{\mu}g/ml$ concentration. At $50{\mu}g/ml$ concentration, the procollagen biosynthesis effect of ethanol extract from S. china L. was 139.86%. At the same concentration, the matrix metalloprotease (MMP)-1 inhibition effect of the ethanol extract was 74.9%. According to the results of Western blot of ethanol extract from S. china L., the expression of the MMP-1 protein was decreased by 35% at $50{\mu}g/ml$ concentration. Reverse transcription-polymerase chain reaction (PCR) of ethanol extract from S. china L. showed that the expression of MMP-1 mRNA was decreased by 45% at $50{\mu}g/ml$ concentration. The findings suggest that 70% ethanol extract from S. china L. (SC) has great potential as a cosmeceutical ingredient with antiwrinkle effects.
Keywords
Anti-wrinkle; MMP-1; pro-collagen; Smilax china L.;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Cannell, R. J., Kellan, S. J., Owsianks, A. M. and Walker, J. M. 1988. Results of a large scale screen of microalgae for the production of protease inhibitors. Planta Med 54, 10-14.   DOI   ScienceOn
2 Carmichael, J., DeGraff, W. G., Gazdar, A. F., Minna, J. D. and Mitchell, J. B. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res 47, 936-942.
3 El-Domyati, M., Attia, S., Saleh, F., Brown, D., Birk, D. E., Gasparro, F., Ahmad, H. and Uitto, J. 2002. Intrinsic aging vs. photoaging: a comparative histopathological, immunohistochemical, and ultrastructural study of skin. Exp Dermatol 11, 398-405.   DOI   ScienceOn
4 Giacomoni, P. U. and Rein, G. 2001. Factors of skin ageing share common mechanisms. Biogerontology 2, 219-229.   DOI   ScienceOn
5 Gross, J. and Lapiere, C. M. 1962. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci USA 54, 1197-1204.
6 Jeroma, S. P., Gabrielle, L. and Raul, F. 1998. Identification of collagen fibrils in scleroderma skin. J Invest Dermatol 90, 48-54.
7 Kim, D. H. 2011. The study on verification of skin pharmacological mechanism from useful compound of Aruncus dioicus and special cosmetic formular. Ph. D. dissertation, Daegu Haany University, Gyeongbuk, Korea.
8 Kondo, S. 2000. The roles of cytokines in photoaging. J Dermatol Sci 23, 30-36.   DOI   ScienceOn
9 Lee, J. Y., Park, W. S., Son, E. D., Ahn, S. M. and Hwang, J. S. 2007. In vitro evalution methods for the development of cosmeceutical ingredients. J Alternat Animal Exp 1, 33-40.
10 Lim, A. K., Kim, J. O., Jung, M. J., Jung, H. K., Hong, J. H. and Kim, D. I. 2008. Functional biological activity of hot water and ethanol extracts from Taraxaci Herba. J Korean Soc Food Sci Nutr 37, 1231-1237.   과학기술학회마을   DOI   ScienceOn
11 Nagase, H. and Woessner, J. F. Jr. 1999. Matrix metalloproteinase. J Biol Chem 274, 21491-21494.   DOI
12 Seo, I. A. 2010. Studies on cosmeceutical of Phaseolus radiatus. M. S. dissertation, Daegu Haany University, Gyeongbuk, Korea.
13 Shao, B., Guo, H., Cui, Y., Ye, M., Han, J. and Guo, D. 2007. Steroidal saponins from Smilax china and their anti-inflammatory activities. Phytochemistry 68, 623-630.   DOI   ScienceOn
14 Song, H. S., Park, Y. H., Jung, S. H., Kim, D. P., Jung, Y. H., Lee, M. K. and Moon, K. Y. 2006. Antioxidant activity of extracts from Smilax china Root. J Korean Soc Food Sci Nutr 35, 1133-1138.   과학기술학회마을   DOI   ScienceOn
15 Talwar, H. S., Griffiths, C. E., Fisher, G. J., Hamilton, T. A. and Voorhees, J. J. 1995. Reduced type I and type III procollagens in photodamaged adult human skin. J Invenst Dermatol 105, 285-290.   DOI   ScienceOn
16 Wang, Y., Johnson, A. R., Ye, Q. Z. and Dyer, R. D. 1999. Catalytic activities and substrate specificity of the human membrane type 4 matrix metalloproteinase catalytic domain. J Biol Chem 274, 33043-33049.   DOI
17 Wlaschek, M., Tantcheva-Poor, I., Naderi, L., Ma, W., Schneider, L. A., Razi-Wolf, Z., Schuller, J. and Scharffetter-Kochanek, K. 2001. Solar UV irradiation and dermal photoaging. J Photochem Photobiol B 63, 41-51.   DOI   ScienceOn
18 Wu, L. S., Wang, X. J., Wang, H., Yang, H. W., Jia, A. Q. and Ding, Q. 2010. Cytotoxic polyphenols against breast tumor cell in Smilax china L. J Ethnopharmacol 130, 460-464.   DOI   ScienceOn
19 Wunsch, E. and Heindrich, H. G. Zur. 1963. Quantitativen bestimmung der collagenase. Hoppe-Seyler's Physiol Chem 333, 149-151.   DOI
20 Yang, K. H. 2004. Studies on the antioxidation and antimicrobial effect of smilacis glabrae rhizome. J Korean Oriental Med 25, 1-8.   과학기술학회마을