• 한국식품저장유통학회지
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• 제20권5호
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• pp.691-698
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• 2013

식품 연구에서 흔히 사용되는 물과 주정(70% 에탄올)을 사용하여 추출된 발계(SCR)의 열수 추출물(SCRW)과 에탄올 추출물(SCRW)의 총 플라보노이드 및 폴리페놀 함량, 항산화 활성, 항암활성을 측정하였다. SCRW와 SCRE는 DPPH(SCRW; 72.9%, SCRE; 42.4%)와 ABTS(SCRW; 81.6%, SCRE; 50.3%) 자유 라디칼을 효과적으로 소거하였으며, 양성 대조군인 BHA(53.8% DPPH, 49.5% ABTS)보다 더 높은 소거활성을 나타냈다. 또한 인체 유래 신장 정상세포(HEK293)에 대해서는 높은 생존율을 나타내 독성이 없음을 확인한 반면, 인체 유래의 암세포(유방암세포; MCF-7, 폐암세포; A549, 위암세포; AGS)에 대해서는 대조군으로 사용된 항암제 CPA와 유사하거나 더 높은 세포성장 억제효과가 확인되었다. 특히 SCRE는 AGS에 대하여 $1,000{\mu}g/mL$ 농도에서 53.6%로 탁월한 위암 세포 성장저해 효과를 나타냈다. 본 연구 결과를 통해, 발계 추출물의 높은 항산화 및 항암 활성이 확인되어 약리활성에 대한 기초연구 자료로 제시 할 수 있을 것으로 사료되며, 기능성 소재로써의 이용 가치를 높일 수 있을 것으로 판단된다.

• 생명과학회지
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• 제25권4호
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• pp.450-455
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• 2015

본 연구에서는 국내에서 주로 소비되는 국내산 및 수입산 참돔의 일반성분과 중금속 함량을 비교 분석하였고 생리활성을 비교하기 위하여 참돔을 용매의 극성에 따라 추출하여 항산화 및 세포독성 효과에 대해 연구하였다. 수입산과 비교했을 때 국내산 참돔은 높은 함량의 수분과 낮은 함량의 조지방, 조단백질 및 회분을 나타내었다. 국내산 및 수입산 참돔의 중금속 함량을 비교한 결과 원산지에 따른 유의적 차이를 보였으며 국내산 참돔의 수은 함량은 0.08 mg/kg으로 수입산 참돔보다 높은 함량을 나타내었으나 납의 경우 원산지에 따른 유의적 차이는 보이지 않았다. 국내산 및 수입산 참돔의 A+M 및 MeOH 추출물을 인체 섬유육종세포(HT-1080)에 처리하였을 때, 세포 내 활성산소종 생성 저해효과를 살펴 본 실험 결과, 국내산 및 수입산 참돔 추출물들에 의한 활성산소종 생성 억제효과를 비교해 보면 수입산 참돔 추출물에 의한 저해효과가 높았으며 두 추출물들 중 MeOH 추출물에 의한 저해효과가 높았음을 알 수 있었다. 인체 암세포들(AGS 및 HT-29)들에 대한 세포독성 활성 결과에서 AGS 암세포의 경우 국내산 참돔 A+M 및 MeOH 추출물들에 의한 세포독성 효과가 수입산 참돔A+M 추출물들보다 높았다. HT-29 암세포에 대해서는 국내산 참돔A+M 추출물에 의한 세포독성 효과가 수입산 참돔 A+M 추출물에 의한 것보다 높았고 MeOH 추출물에 의한 세포독성 효과는 유사했다. 따라서 본 연구 결과를 기초자료로 하여 향후 참돔의 다양한 생리활성 규명이 필요하다고 사료된다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4241-4244
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• 2012

The lung adenocarcinoma is considered more aggressive than other types of non-small cell lung cancer. As metabolites of tryptophan degradation along the kynurenine pathway, including kynurenic acid, have been shown to induce immunosuppression and facilitate escape of tumor cells from immune surveillance, a hypothesis was set up that differences in biological behavior between types of lung cancer may be associated with altered activity of the kynurenine metabolic pathway. The aim of the study was to determine kynurenic acid levels in the serum of patients with bronchial adenocarcinoma for comparison with other types of non-small cell lung cancer. A total of 227 patients with non-small cell lung cancer were enrolled in the study, including 71 with adenocarcinoma and 96 with squamous cell carcinoma. Serum kynurenic acid concentration was determined with use of high performance liquid chromatography and fluorometry. The level of kynurenic acid in the serum of patients with adenocarcinoma was significantly higher than in those with squamous cell lung cancer ($107.1{\pm}62.8$ pmol/ml; 95%CI: 92.4 to 132.3 pmol/ml versus $82.1{\pm}47.6$ pmol/ml; 95%CI: 78.5 to 91.2 pmol/ml, respectively; p = 0.027). Differences between other histological types of lung cancer were insignificant. We conclude that increased activity of kynurenine metabolic pathway manifested by elevated serum kynurenic acid level may be one of the factors associated with clinically distinct biological behavior of adenocarcinoma, in particular high invasiveness and rapid progression.

• Journal of Korean Neurosurgical Society
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• 제46권6호
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• pp.552-557
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• 2009

Objective : Interferon-$\beta$, (IFN-$\beta$) has been used in the treatment of cancers. Inhibition of the enzyme cyclooxygenase (COX) with celecoxib had a significantly suppressive effect on tumor growth, angiogenesis, and metastasis in a variety of tumors. The aim of this study was to elucidate the antiglioma effect of combined treatment with IFN-$\beta$ and celecoxib in U87 glioma model. Methods : The in vitro effects of IFN-$\beta$ (50-1,000 IU/mL) and celecoxib ($50-250\;{\mu}M$) alone or combination of both on the proliferation and apoptosis of U87 cells were tested using MTT assay, FACS analysis and DNA condensation. To determine the in vivo effect, nude mice bearing intracerebral U87 xenograft inoculation were treated with IFN-$\beta$ intraperitoneally ($2{\times}10^5\;IU/day$ for 15 days), celecoxib orally (5, 10 mg/kg) or their combination. Results : IFN-$\beta$ or celecoxib showed an inhibitory effect on the proliferation of U87 cells. When U87 cells were treated with IFN-$\beta$ and celecoxib combination, it seemed that IFN-$\beta$ interrupted the antiproliferative and apoptotic activity of celecoxib. No additive effect was observed on the survival of the tumor bearing mice by the combination of IFN-$\beta$ and celecoxib. Conclusion : These results suggest that IFN-$\beta$ seems to inhibit the antiglioma effect of celecoxib, therefore combination of IFN-$\beta$ and celecoxib may be undesirable in the treatment of glioma.

• Asian Pacific Journal of Cancer Prevention
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• 제16권14호
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• pp.5697-5701
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• 2015

Background: Medicinal plants, especially examples rich in polyphenolic compounds, have been suggested to be chemopreventive on account of antioxidative properties. Punica granatum (PG) (pomegranate) is a well known fruit in this context, but its cytotoxicity in cancer cells has not been extensively studied. Here, we investigated the antiproliferative properties of a peel extract of PG from Iran in different human cancer cells. Materials and Methods: A methanolic extract of pomegranate peel (PPE) was prepared. Total phenolic content(TPC) and total flavonoid conetnt (TFC) were determined by colorimetric assays. Antioxidant activity was determined by DPPH radical scavenging activity. The cytotoxicity of different doses of PPE (0, 5, 20, 100, 250, 500, $1000{\mu}g/ml$) was evaluated by MTT assays with A549 (lung non small cell cancer), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer), and PC-3 (prostate adenocarcinoma) cells. Results: Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison with negative controls at all tested doses (5-$1000{\mu}g/ml$). In all studied cancer cells, PPE reduced the cell viability to values below 40%, even at the lowest doses. In all cases, IC50 was determined at doses below $5{\mu}g/ml$. In this regard, MCF-7 breast adenocarcinoma cells were the most responsive cells to antiprolifreative effects of PPE with a maximum mean growth inhibition of 81.0% vs. 69.4%, 79.3% and 77.5% in SKOV3, PC-3 and A549 cells, respectively. Conclusions: Low doses of PPE exert potent anti-proliferative effects in different human cancer cells and it seems that MCF-7 breast adenocarcinoma cells are the most cells and SKOV3 ovarian cancer cells the least responsive in this regard. However, the mechanisms of action need to be addressed.

• Asian Pacific Journal of Cancer Prevention
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• 제16권10호
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• pp.4303-4310
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• 2015

Rumex vesicarius is an edible herb distributed in Egypt and Saudi Arabia. The whole plant has significant value in folk medicine and it has been used to alleviate several diseases. Hepatocellular carcinoma (HCC), the major primary malignant tumor of the liver, is one of the most life-threatening human cancers. The goal of the current study was to explore the potent role of Rumex vesicarius extract against HCC induced in rats. Thirty adult male albino rats were divided into 3 groups: (I): Healthy animals received orally 0.9 % normal saline and served as negative control group, (II): HCC group in which rats were orally administered N-nitrosodiethylamine NDEA, (III): HCC group treated orally with R. vesicarius extract in a dose of 400 mg/kg b.wt daily for two months. ALT and AST, ALP and ${\gamma}$-GT activities were estimated. CEA, AFP, AFU, GPC-3, Gp-73 and VEGF levels were quantified. Histopathological examination of liver tissue sections was also carried out. The results of the current study showed that the treatment of the HCC group with R. vesicarius extract reversed the significant increase in liver enzymes activity, CEA, AFP, AFU, glypican 3, golgi 73 and VEGF levels in serum as compared to HCC-untreated counterparts. In addition, the favorable impact of R. vesicarius treatment was evidenced by the marked improvement in the histopathological features of the liver of the treated group. In conclusion, the present experimental setting provided evidence for the significance of R. vesicarius as anticancer candidate with a promising anticancer potential against HCC. The powerful hepatoprotective properties, the potent antiangiogenic activity and the effective antiproliferative capacity are responsible for the anticancer effect of this plant.

• Clinical and Experimental Reproductive Medicine
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• 제48권4호
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• pp.322-336
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• 2021

Objective: Endometriosis is a chronic debilitating inflammatory condition characterized by the presence of endometrial tissues outside the uterine cavity. Pelvic soreness and infertility are the usual association. Due to the poor effectiveness of the hormone therapy and the high incidence of recurrence following surgical excision, there is no single effective option for management of endometriosis. Mesenchymal stem cells (MSCs) are multipotent stromal cells studied for their broad immunoregulatory and anti-inflammatory properties; however, their efficiency in endometriosis cases is still a controversial issue. Our study aim was to evaluate whether adipose tissue-derived MSCs (AD-MSCs) could help with endometriosis through their studied anti-inflammatory role. Methods: Female Wistar rats weighting 180 to 250 g were randomly divided into two groups: group 1, endometriosis group; established by transplanting autologous uterine tissue into rats' peritoneal cavities and group 2, stem cell treated group; treated with AD-MSCs on the 5th day after induction of endometriosis. The proliferative activity of the endometriosis lesions was evaluated through Ki67 staining. Quantitative estimation of interferon γ, tumor necrosis factor-α, interleukin (IL)-6, IL-1β, IL-10, and transforming growth factor β expression, as well as immunohistochemical detection of CD68 positive macrophages, were used to assess the inflammatory status. Results: The size and proliferative activity of endometriosis lesions were significantly reduced in the stem cell treated group. Stem cells efficiently mitigated endometriosis associated chronic inflammatory reactions estimated through reduction of CD68 positive macrophages and the expression of the proinflammatory cytokines. Conclusion: Stem cell therapy can be considered a novel remedy in endometriosis possibly through its anti-inflammatory and antiproliferative properties.

• 산업식품공학
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• 제15권1호
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• pp.64-69
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• 2011

본 실험에서는 대장암 세포 HT-29의 증식을 억제하고 세포 사멸을 유도하는 천연소재 발굴을 목적으로, 오미자(Schizandra chinensis Baillon) 열수 추출물을 이용하여 인체대장암 세포 HT-29의증식에 미치는 영향을 확인하였다. 오미자 열수 추출물이 HT-29 대장암 세포의 apoptosis 유도 효과 및 기전에 미치는 영향을 분자생물학적 방법으로 실험하여 다음과 같은 결론을 얻었다. MTT assay를 통해 인체 대장암세포 HT-29는 오미자 시료농도 0, 1.0, 2.0, 4.0 mg/mL에서 암세포 사멸농도가 각각 0%, 10%, 70%, 88%를 나타내었다. 대장암세포에 오미자 추출물을 처리하고 cell cycle 분석 결과, 시료농도 의존적으로 sub-G1기가 증가하였고, G0/G1기는 감소되는 것을 통해, apoptosis가 일어나 세포 증식을 저해하는 것으로 확인되었다. 대장암세포 핵의 형태학적 변화를 보면, 오미자 추출물 처리 시 농도 의존적으로 세포수가 감소되는 것이 뚜렷이 관찰되었고 cell shrinking, chromatin condensation등 apototic body 등과 같은 형태학적 변화들이 뚜렷하게 관찰되었다. RT- PCR을 통한 유전자 발현은, 오미자 추출물 농도 의존적으로 p53 유전자 발현이 증가되는 것을 통해 대장암세포의 증식억제를 확인할 수 있었다. In vitro 실험에서 오미자 열수추출물이 대장암세포의 성장을 저해하는 효과가 있음을 확인하였으며, 오미자 추출물에 함유된 활성 본체 규명 및 apoptosis를 유도하는 작용기작에 관한 연구가 수행중이다.

• Biomolecules & Therapeutics
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• 제31권6호
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• pp.661-673
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• 2023

Treatment of colorectal cancer (CRC) has always been challenged by the development of resistance. We investigated the antiproliferative activity of licochalcone H (LCH), a regioisomer of licochalcone C derived from the root of Glycyrrhiza inflata, in oxaliplatin (Ox)-sensitive and -resistant CRC cells. LCH significantly inhibited cell viability and colony growth in both Ox-sensitive and Ox-resistant CRC cells. We found that LCH decreased epidermal growth factor receptor (EGFR) and AKT kinase activities and related activating signaling proteins including pEGFR and pAKT. A computational docking model indicated that LCH may interact with EGFR, AKT1, and AKT2 at the ATP-binding sites. LCH induced ROS generation and increased the expression of the ER stress markers. LCH treatment of CRC cells induced depolarization of MMP. Multi-caspase activity was induced by LCH treatment and confirmed by Z-VAD-FMK treatment. LCH increased the number of sub-G1 cells and arrested the cell cycle at the G1 phase. Taken together LCH inhibits the growth of Ox-sensitive and Ox-resistant CRC cells by targeting EGFR and AKT, and inducing ROS generation and ER stress-mediated apoptosis. Therefore, LCH could be a potential therapeutic agent for improving not only Ox-sensitive but also Ox-resistant CRC treatment.

• 한국식품저장유통학회지
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• 제23권7호
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• pp.1033-1041
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• 2016

본 논문에서는 조, 기장 및 수수 추출물에 대한 항산화활성 및 인체 전립선암 세포성장 억제효과를 평가하고자 하였다. 조, 기장 및 수수 추출물에 대한 항산화 활성을 DPPH 라디칼 소거능, ABTS 라디칼 소거능, 환원력 및 SOD 유사 활성능을 통해 확인한 결과, 수수 추출물에서 농도의존적인 항산화 활성의 증가를 관찰할 수 있었으며 이는 조 및 기장 추출물보다 높은 효능을 보이는 것으로 나타났다. 두 가지의 다른 형태의 인체 전립선 암세포(PC-3 및 LNCaP)를 이용하여 암세포 증식 억제활성을 확인한 결과, 조 추출물과 기장 추출물의 처리는 $1,000{\mu}g/mL$의 농도에서도 큰 억제 효과를 확인할 수 없었으나 LNCaP 세포에 수수 추출물을 처리한 군에서는 $10{\mu}g/mL$의 농도에서부터 유의적인 세포증식 억제효능을 확인할 수 있었다. PC-3 및 LNCaP 세포의 형태학적 변화를 알아본 결과, 수수 추출물을 처리한 군에서 부유, 사멸 및 증식억제가 관찰되었으나 동일한 농도의 조 및 기장 추출물을 처리한 군에서는 대조군과 유사한 세포형태를 유지하였으며 특이적인 세포독성이 관찰되지 않았다. Hoechst 염색을 통한 핵 형태 관찰에서도 조 및 기장 추출물과는 대조적으로 수수 추출물에서만 apoptotic body, 세포 및 핵의 응축 등 세포 사멸과 관련된 형태학적 변화가 관찰되었다. 조, 기장, 수수 추출물의 총 폴리페놀 함량은 각각 2.01, 1.71, 29.10 mg GAE/g로 나타났으며, 총 폴리페놀 함량이 가장 높았던 수수에서 항산화 및 항암 효능이 우수함을 확인할 수 있었다. 결과적으로 조, 기장 및 수수 추출물 중 폴리페놀 함량이 가장 높았던 수수에서 항산화 활성 및 인체 전립선 암세포의 성장 억제효과가 뛰어난 것으로 확인되었으며, 이를 통해 수수 추출물을 항산화 및 암세포의 증식억제를 위한 식품원료 및 산업 소재로 활용할 수 있을 것으로 사료된다.