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http://dx.doi.org/10.5352/JLS.2015.25.4.450

Heavy Metal Contents and Antioxidant Activity and Cytotoxic Effect of Red Sea Bream (Pagrus major): Comparative Studies in Domestic and Imported Red Sea Bream (Pagrus major)  

Hwang, Seong Yeon (Division of Marine Bioscience, Korea Maritime and Ocean University)
Bae, Jin Han (Busan Regional Food & Drug Administration, Ministry of Food and Drug Safety)
Lim, Sun-Young (Division of Marine Bioscience, Korea Maritime and Ocean University)
Publication Information
Journal of Life Science / v.25, no.4, 2015 , pp. 450-455 More about this Journal
Abstract
This study compared the heavy metal contents and the effects of extracts from domestic and imported red sea bream on the antioxidant activity and cytotoxicity of human cancer cell lines. The antioxidant activity was measured using the fluorescently sensitive dye, 2’-7’ dichlorofluorescein-diacetate (DCFH-DA), and antiproliferative activity against AGS human gastric adenocarcinoma and HT-29 human colon cancer cell lines, which was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Domestic red sea bream had a higher mercury content when compared to imported red sea bream, but there was no significant difference in the lead content. Treatments with acetone/methylene chloride (A+M) and methanol (MeOH) extracts from domestic and imported red sea bream dose-dependently decreased the H2O2 induced ROS production, compared to the control. The cell viability showed that treatments with the A+M and MeOH extracts had cytotoxicity in the growth of AGS and HT-29 cancer cells. In the case of AGS, the extracts from the domestic red sea bream were higher in inhibiting cancer cell growth, compared to imported red sea bream. Our results demonstrate that the heavy metal contents of domestic and imported red sea bream were below the limit of the Food Code of Korea. The results of the biological activities indicate that the antioxidant activity of extracts from imported red sea bream was more effective, while the extracts from the domestic red sea bream were stronger in cytotoxic activity.
Keywords
Antioxidant; cytotoxicity; heavy metals; reactive oxygen species; red sea bream;
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