Food Engineering Progress (산업식품공학)

Korean Society for Industrial Food Engineering (한국산업식품공학회)
권호 표지

Effects on Hot Water Extract of Schizandra chinensis on Colon Cancer

오미자 열수추출물의 대장암세포 증식억제 효과

  • Ryu, Min-Ju (Department of Food and Nutrition, Duksung Women's University) ;
  • Chung, Ha-Sook (Department of Food and Nutrition, Duksung Women's University)
  • 유민주 (덕성여자대학교 식품영양학과) ;
  • 정하숙 (덕성여자대학교 식품영양학과)
  • Received : 2011.01.31
  • Accepted : 2011.02.21
  • Published : 2011.02.28
⟨ Previous Next ⟩

Abstract

The anticancer activity of Schizandra chinensis Baillon was investigated for the development of functional food resources. The antiproliferative activity of hot water extracts of Schizandra chinensis Baillon in human colon cancer cell line (HT-29) were identified using cell viability, morphology study, cell cycle and RT-PCR analyses. HT-29 cells were cultured in several concentrations (0, 1.0, 2.0, 4.0 mg/mL) of water extracts of Schizandra chinensis Baillon. In our study, colon cancer cell growth could be inhibited by hot water extracts of Schizandra chinensis Baillon in a dose-dependent manners. It was associated with morphological changes and apoptotic cell death with cell shrinking, chromatin condensation, apoptotic bodies and cell cycle analysis. These results suggest that Schizandra chinensis Baillon may inhibit the growth of human colon cancer cells by various apoptosis-aiding activities as well as apoptosis itself.

본 실험에서는 대장암 세포 HT-29의 증식을 억제하고 세포 사멸을 유도하는 천연소재 발굴을 목적으로, 오미자(Schizandra chinensis Baillon) 열수 추출물을 이용하여 인체대장암 세포 HT-29의증식에 미치는 영향을 확인하였다. 오미자 열수 추출물이 HT-29 대장암 세포의 apoptosis 유도 효과 및 기전에 미치는 영향을 분자생물학적 방법으로 실험하여 다음과 같은 결론을 얻었다. MTT assay를 통해 인체 대장암세포 HT-29는 오미자 시료농도 0, 1.0, 2.0, 4.0 mg/mL에서 암세포 사멸농도가 각각 0%, 10%, 70%, 88%를 나타내었다. 대장암세포에 오미자 추출물을 처리하고 cell cycle 분석 결과, 시료농도 의존적으로 sub-G1기가 증가하였고, G0/G1기는 감소되는 것을 통해, apoptosis가 일어나 세포 증식을 저해하는 것으로 확인되었다. 대장암세포 핵의 형태학적 변화를 보면, 오미자 추출물 처리 시 농도 의존적으로 세포수가 감소되는 것이 뚜렷이 관찰되었고 cell shrinking, chromatin condensation등 apototic body 등과 같은 형태학적 변화들이 뚜렷하게 관찰되었다. RT- PCR을 통한 유전자 발현은, 오미자 추출물 농도 의존적으로 p53 유전자 발현이 증가되는 것을 통해 대장암세포의 증식억제를 확인할 수 있었다. In vitro 실험에서 오미자 열수추출물이 대장암세포의 성장을 저해하는 효과가 있음을 확인하였으며, 오미자 추출물에 함유된 활성 본체 규명 및 apoptosis를 유도하는 작용기작에 관한 연구가 수행중이다.

Keywords

Acknowledgement

Supported by : 덕성여자대학교

References

  1. Donald MW, Nell GH. 1984. Biological activities of lignans. Phytochem. 23(12): 1207-1213.
  2. Hertog MG, Hollman PC, Katan MB, Kromhout D. 1993. Intake of potentially anticarcinogenic flavonoids and their determinants in adults in The Netherlands. Nutr. Cancer. 20(1): 21-29. https://doi.org/10.1080/01635589309514267
  3. Hong-Yen H, Yuhp-Pan C, Shuenn-Jyi S, Chau-Shin H, Chien-Chin C, Sien-Chang C. 1986. Oriental Materia Medica, Oriental Healing Arts Institute, California, UKroemer G. 1997. The proto-oncogene Bcl-2 and its role in regulating apoptosis. Nat. Med. 3: 614-620. SA, 624.
  4. Krishnaswamy K, Raghuramulu N. 1998. Bioactive phytochemicals with emphasis on dietary practices. Indian J. Med. Res. 108(4): 167-181.
  5. Kronborg O, Fenger C, Olsen J, Jorgensen OD, Sondergaard O. 1996. Randomized study of screening for colorectal cancer with faecaloccult blood test. Lancet. 348(12): 1467-1471.
  6. Lee KS, Hwang CS. 1990. A study on the actual utilization Korean traditional remedies about foods used on geriatric disease. Korean J. Dietary Culture 5(4): 331-347.
  7. Mok C. 2005. Quality characteristics of Instant Tea prepared from spray-dried Omija(Schizandra chinensis Baillon) extracts/grape juice mixture. Food Eng. Prog. 9(3): 226-230.
  8. Oh SL, Kim SS, Min BY, Chung DH. 1990. Composition of free sugars, free anion acids, non-volatile organic acids and tannins in the extracts of L. Chinensis M., A. acutiloba K., S. chinensis B. and A. sessiliflorum S. Korean J. Food Sci. Technol. 22(2): 76-81.
  9. Prescott SM, Fitzpatrick FA. 2000. Cyclooxygenase-2 and carcinogenesis. Biochim. Biophys. Acta. 1470: M69-M78.
  10. Reed JC. 1998. Bcl-2 family proteins. Oncogene 17: 3225-3236.
  11. Surh Y. 1999. Molecular mechanisms of chemopreventive effects of selecteddietary and medicinal phenolic substances. Mutat Res. 428(1-2): 305-327. https://doi.org/10.1016/S1383-5742(99)00057-5
  12. Vogelstein BD, Levine AJ. 2005. Surfing the p53 network. Nature 408(12): 307-310.
  13. Winawer SJ, St John DJ and Bond JH. 1996. Prevention of colorectal carcinoma. Current WHO guidelines for early detection of colorectal carcinoma. World Health Organization Collaborating Center for the Prevention of Colorectal Cancer. Leber Magen Darm. 26(43): 139-140.
  14. Yoon YS, Yu CS, Jung SH, Choi PW, Han KR, Kim HC, Kim JC. 2007. Characteristics of colorectal cancer detected at the Health Promotion Center. J. Korean Soc. Coloproctol. 23(6): 321-326.