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http://dx.doi.org/10.11002/kjfp.2013.20.5.691

Antioxidant and anticancer properties of hot water and ethanol extracts from the roots of Smilax china L.  

Kim, Ye Jin (Department of Food Science and Nutrition, Kyungpook National University)
Son, Dae-Yeul (Department of Herbal Food Science, Daegu Haany University)
Publication Information
Food Science and Preservation / v.20, no.5, 2013 , pp. 691-698 More about this Journal
Abstract
The biological activities of Smilax china L. rhizome (SCR), hot water (SCRW) and 70% ethanol extract (SCRE) were analyzed. The total phenolic contents of SCRW and SCRE were 51.7 and 100.5 mg/g, respectively. The measured flavonoid content of SCRW ($67.7{\mu}g/g$) was almost double that of SCRE ($31.7{\mu}g/g$). SCRE ($IC_{50}=42.4{\mu}g/mL$) exhibited stronger antioxidant activity in the DPPH system than the positive control ${\alpha}$-tocopherol ($71.3{\mu}g/mL$) or butylated hydroxy anisole ($53.8{\mu}g/mL$) did. SCRE ($IC_{50}=50.3{\mu}g/mL$) also showed stronger ABTS radical scavenging activity, as did ${\alpha}$-tocopherol ($67.1{\mu}g/mL$). The SOD-like activity and Tyrosinase inhibition activity of SCRW and SCRE showed almost the same pattern. The best SOD-like activity and tyrosinase inhibition activity were measured as 24.9% and 20.3% in SCRW at $1,000{\mu}g/mL$, respectively. The cytotoxic effects of the SCR extracts were analyzed via MTT assay on human cancer and normal cells. SCRW and SCRE did not show cytotoxicity up to the concentration of $1,000{\mu}g/mL$ against the normal human cell line HEK293. Against human breast cancer cells (MCF-7), SCRW inhibited MCF-7 growth (by 27.6%) better than the anticancer drug cyclophosphamide (15.5%) at $1,000{\mu}g/mL$. SCRE ($1,000{\mu}g/mL$) inhibited the growth of human lung cancer cells A549 (37.6%) and human stomach cancer cells AGS (53.6%) more effective than did SCRW (21.0% and 35.4%) or CPA (22.2% and 31.7%). These results suggest the potential use of SCRE and SCRW as an excellent antioxidant and antiproliferative substance, respectively.
Keywords
anticancer; antioxidant; 1,1-diphenyl-2-picrylhydrazyl; polyphenol; Smilax china L.;
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Times Cited By KSCI : 14  (Citation Analysis)
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1 Tepe B, Sokmen A (2007) Screening of the antioxidative properties and total phenolic contents of three endemic Tanacetum subspecies from Turkish flora. Bioresour Technol, 98, 3076-3079   DOI   ScienceOn
2 Lee SK, Jiang CK, Jeong KJ, Park DK, Paik HD, Yoon YC (2008) Antioxidant effects of Cheonggukjang containing Plellinus linteus extract. Food Sci Biotechnol, 17, 85-89
3 Jeon JR, Jin T, Kim J, Park JR (2006) Chemical composition of Smilax china leaves and quality characteristics of rice cakes prepared with its water extract. Food Science Biotechnol, 15, 606-611   과학기술학회마을
4 Wu LS, Wang XJ, Wang H, Yang HW, Jia AQ, Ding QA (2010) Cytotoxic polyphenols against breast tumor cell in Smilax china L. J Ethnopharmacol, 130, 460-464   DOI   ScienceOn
5 Shu XS, Gao ZH, Yang XL (2006) Anti-inflammatory and anti-nociceptiveb activities of Smilax china L. aqueous extract. J Ethnopharmacol, 103, 327-332   DOI   ScienceOn
6 Bo S, Hongzhu G, Yajun C, Min Y, Jian H, Dean G (2007) Steroidal saponins from Smilax china and their anti-inflammatory activities. Phytochem, 68, 623-630   DOI   ScienceOn
7 Jin TY, Park JR, Kim JH (2004) Electron donating abilities nitrite scavenging effects antimicrobial activities of Smilax china leaf. J Korean Soc Food Sci Nutr, 33, 621-625   과학기술학회마을   DOI   ScienceOn
8 Liang C, Lim JH, Kim SH, Kim DS (2012) Dioscin: a synergistic tyrosinase inhibitor from the roots of Smilax china. Food Chem, 134, 1146-8   DOI   ScienceOn
9 Xu W, Liu JW, Li CL, Wu HZ, Liu YW (2008) Kaempferol-7-O-beta-D-glucoside (KG) isolated from Smilax china L. rhizome induces G(2)/M phase arrest and apoptosis on HeLa cells in a p53-independent manner. Cancer Lett, 264, 229-240   DOI   ScienceOn
10 Vijayalakshmi A, Ravichandiran V, Malarkodi V, Nirmala S, Jayakumari S (2012) Screening of flavonoid "quercetin" from the rhizome of Smilax china Linn. for anti-psoriatic activity. Asian Pac J Trop Biomed, 269-275
11 Folin O, Denis W (1912) On phosphotungastic phosphomolybdic compounds as color reagents. J Biol Chem, 12, 239-249
12 Pinelo M, Rubilar M, Jerez M, Sineiro J, Nunez MJ (2005) Effect of solvent, temperature, and solvent-to solid ratio on the total phenolic content and antiradical activity of extracts from different components of grape pomace. J Agric Food Chem, 53, 2111-2117   DOI   ScienceOn
13 Tsao R (2010) Chemistry and biochemistry of dietary polyphenols. Nutrients, 2, 1231-1246   DOI
14 Lee SG, Choi SW, Lee EJ, Kim PJ (2011) Antioxidant and anti-inflammatory activities of extracts from Korean traditional medicinal prescriptions. Korean J Food Sci Technol, 43, 624-632   과학기술학회마을   DOI   ScienceOn
15 Park YS (2002) Antioxidative activities and contents of polyphenolic compound of medicinal herb extracts. J East Asian Soc Dietary Life, 12, 23-31   과학기술학회마을
16 Whang HJ, Han WS, Yoon KR (2001) Quantitative analysis of total phenolic content in apple. Analyt Sci Technol, 14, 377-383   과학기술학회마을
17 Williams RJ, Spencer JP, Rice-Evans C (2004) Flavonoids: Antioxidants or signaling molecules? Free Radic Biol Med, 36, 838-849   DOI   ScienceOn
18 Sohn HY, Ryu HY, Jang YJ, Jang HS, Park YM, Kim SY (2008) Evaluation of antimicrobial, antithrombin, and antioxidant activity of aerial part of Saxifraga stolonifera. Korean J Microbiol Biotechnol, 36, 195-200   과학기술학회마을
19 Kwon HJ, Park CS (2008) Biological activities of extracts from Omija. Korean J Food Preserv, 15, 587-592   과학기술학회마을
20 Lee HS, Yang HJ, Park MJ (2011) Antioxidative activities and components of Gardenia jasminoides. Korean J Food Sci Technol, 43, 51-57   과학기술학회마을   DOI   ScienceOn
21 Yi O, Jovel EM, Towers NGH, Wahbe TR, Cho D (2007) Antioxidant and antimicrobial activities of native Rosa sp. from British Columbia Canada. Int J Food Sci Nutr, 58, 178-189   DOI   ScienceOn
22 Lee HS, Cho MJ, Park MJ (2007) Nitrite scavenging ability and SOD-Like activity of a sterol glucoside from Chrysanthemum coronarium L. var. spatiosum. Korean J Food Sci Technol, 39, 77-82   과학기술학회마을
23 Kim NW, Lee YS, Yoon HG (2010) The physiological activities of ripe fruit of Poncirus trifoliata. Korean J Food Preserv, 17, 698-705   과학기술학회마을
24 Kim SN, Kim JS, Ahn JY, Ha TY, Rhee HC (2011) Comparison of phytochemical and antioxidant activities in different color stages and varieties of paprika harvested in Korea. Korean J Food Sci Technol, 43, 564-569   과학기술학회마을   DOI   ScienceOn
25 Harman D (1956) A theory based on free radical and radiation chemistry. J Gerontol 11, 298-307   DOI   ScienceOn
26 Kim NW, Joo EY (2008) Polyphenol contents and antioxidant activity of extracts from Angelica dahurica root after different conditions of microwave-assisted extraction. Korean J Food Preserv, 15, 133-138   과학기술학회마을
27 Lim JD, Yu CY, Kim MJ, Yun SJ, Lee SJ, Kim NY, Chung IM (2004) Comparison of SOD activity and phenolic compound contents in various Korean medicinal plants. Korean J Med Crop Sci, 12, 191-202
28 Park EM, Bae MJ, Ye EJ, Kim SJ, Kim JM, Yee ST (2007) The effects of plebeiae herba (Salvia plebeian R. Br) on the anticancer (in vitro) and activation of lmmune cells. J Korean Soc Food Sci Nutr, 36, 377-382   DOI   ScienceOn
29 Park YH, Chang SK (1997) Screening of inhibitory effect of edible mushrooms on tyrosinase and isolation of active component. J Food Hyg Safety, 12, 195-1999   과학기술학회마을
30 Lee YS, Chio BD, Joo EY, Shin SR, Kim NW (2009) Antioxidative activities and tyrosinase inhibition ability in various extracts of the Vitex rotundifolia seed. Korean J Food Preserv, 16, 101-108
31 Kim HK, Lee HJ, Do JR, Kwon JH (2010) Antioxidant effects of Viscum album L. extraction conditions. Korean J Soc Food Sci Nutr, 39, 14-19   과학기술학회마을   DOI   ScienceOn
32 Jumeri, Kim SM (2011) Antioxidant and anticancer activities of enzymatic hydrolysates of solitary tunicate (Styela clava). Food Sci Biotechnol, 24, 1075-1085   과학기술학회마을   DOI
33 Khan I, Nisar M, Ebad F, Nadeem S, Saeed M, Khan H, Samiullah, Khuda F, Karim N, Ahmad Z (2009) Anti-inflammatory activities of Sieboldogenin from Smilax china Linn.: experimental and computational studies. J Ethnopharmacol, 121, 175-7   DOI   ScienceOn
34 Young, FM, Phungtamdet W, Sanderson BJS (2005) Modification of MTT assay conditions to examine the cytotoxic effects of amitraz on the human lymphoblastoid cell line, WIL2NS. Toxocol In Vitro, 19, 1051-1059   DOI   ScienceOn