• Title/Summary/Keyword: Anti-platelet

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Anti-thrombotic Effects of Analogs of Protocatechuic Acid and Gallic Acid (Protocatechuic acid 및 Gallic acid 유도체들의 항 혈전작용)

  • 윤혜숙;강삼식;김문희;정기화
    • YAKHAK HOEJI
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    • v.37 no.5
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    • pp.453-457
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    • 1993
  • Platelet anti-aggregating activities were tested with analogs of protocatechuic acid and gallic acid. Six of them which showed comparable inhibitory effects with aspirin against collagen induced platelet aggregation were selected and their anti-thrombotic effects were evaluated in the mouse thrombosis model and compared with those of aspirin and paeonol. At the dose of 50 mg/kg, p.o., ethyl gailate(13) treated group showed higher % of recovery within 6 min of thrombotic challenge and lower mortality within 5 min than aspirin treated group.

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Study on the Inhibition of anti-platelet and Anticoagulant activity from Rhus verniciflua Stokes

  • Kyung, Jeon-Won;Kim, Jung-Hee;Lee, A-Yeong;Kim, Ho-Kyoung
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.271.3-272
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    • 2003
  • Rhus verniciflua Stokes (RVS) is a widely used herbal plant with various biological properties. Our previous study using in vitro platelet aggregation in whole blood showed that the fractions of RVS had strong anti-aggregatory activity. In this study, using in vitro platelet aggregation in PRP and coagulation parameters, to investigate the anti-platelet activity and anticagulant effects of RVS ethyl acetate layer, the layer was subsequently fractionated by ODS columm chromatograph (50% MeOH). (omitted)

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Anti-platelet Effect of Black Tea Extract via Inhibition of TXA2 in Rat

  • Ro, Ju-Ye;Cho, Hyun-Jeong
    • Biomedical Science Letters
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    • v.25 no.4
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    • pp.302-312
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    • 2019
  • The aim of this work was to investigate the effect of black tea extract (BTE) on collagen -induced platelet aggregation. In this study, BTE (10~500 ㎍/mL) was shown to inhibit platelet aggregation via thromboxane A2 (TXA2) down-regulation by blocking cyclooxygenase-1 (COX-1) activity. Also, BTE decreased intracellular Ca2+ mobilization ([Ca2+]i). Additionally, BTE enhanced the levels of both cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP), which are aggregation-inhibiting molecules. BTE inhibited the phosphorylation of phospholipase C (PLC) γ2 and syk activated by collagen. BTE regulated platelet aggregation via cAMP-dependent phosphorylation of vasodilator-stimulated phosphoprotein (VASP) Ser157. The anti-platelet effects of BTE in high fat diet (HFD)-induced obese rats were evaluated. After eight weeks of BTE treatment (300 and 600 mg/kg), the platelet aggregation rate in the treated groups was significantly less than that in the HFD-fed control group. Also, BTE exhibited a hepatoprotective effect and did not exert hepatotoxicity. Therefore, these data suggest that BTE has anti-platelet effects on collagen-stimulated platelet aggregation and may have therapeutic potential for the prevention of platelet-mediated thrombotic diseases.

Platelet Anti-Aggregatory Effects of Coumarins from the Roots of Angelica genuflexa and A. gigas

  • Lee, Yong-Yook;Lee, Sang-Hyun;Jin, Jing-Ling;Yunchoi, Hye-Sook
    • Archives of Pharmacal Research
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    • v.26 no.9
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    • pp.723-726
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    • 2003
  • Five coumarins, isoimperatorin (1), pabulenol (2), isooxypeucedanin (3), oxypeucedanin hydrate (4) and osthol (5) were isolated from the MeOH extract of Angelica genuflexa in the course of searching for anti-platelet and anti-coagulant components from plants. Pabulenol (2) was isolated from A. genuflexa for the first time. The five compounds isolated from A. genuflexa, together with decursinol angelate (6), decursin (7) and nodakenin (8) from A. gigas were evaluated for their effects on platelet aggregation and blood coagulation. Compounds 2, 5, 6 and 7 were observed to be either equally effective or 2∼4 times more inhibitory than ASA in both arachidonic acid and U46619 ($TXA_2$ mimetic) induced platelet aggregations.

Anti-Platelet Aggregation Effect of Extract from Gamisopunghwalheol-tang in Vitro (가미소풍활혈탕의 혈소판 응집억제 작용에 대한 in vitro 연구)

  • Lee, Hae-Yong;Min, Kyoung-Yoon;Kim, Seul-Ji;Park, Youn-Ju;Yang, Ga-Eun;Lee, Mi-Jung;Lew, Jae-Whan;Lee, Beom-Joon;Cho, In-Young
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.23 no.5
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    • pp.980-985
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    • 2009
  • The study was designed to test anti-platelet effect and find out anti-platelet mechanism of extract from Gamisopunghwalheol-tang in vitro. The extract was investigated for the inhibition against the aggregation of human platelet suspensions induced from collagen by aggregometer. And also the extract was investigated for the inhibition against the aggregation of human platelet suspensions who is taking aspirin or clopidogrel induced from collagen by aggregometer. In collagen-induced platelet aggregation test, the extract significantly inhibited collagen-induced platelet aggregation in a concentration-dependent manner(p<0.05). The extract significantly inhibited collagen-induced platelet aggregation of human platelet who is taking aspirin or clopidogrel ing 0 mg/ml concentration(p<0.05). And the extract inhibited more ingpatients who is taking aspirin. These results show that the extract from Gamisopunghwalheol-tang has anti-platelet aggregation effect.

Inhibitory Effects of Yuzu and Its Components on Human Platelet Aggregation

  • Kim, Tae-Ho;Kim, Hye-Min;Park, Se Won;Jung, Yi-Sook
    • Biomolecules & Therapeutics
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    • v.23 no.2
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    • pp.149-155
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    • 2015
  • Our previous study demonstrated that yuzu has an anti-platelet effect in rat blood. In the present study, we examined whether the anti-platelet effect of yuzu can be extended to human blood by investigating its ability to inhibit aggregations induced by various agonists in human platelet rich plasma (PRP). This study also investigated the underlying mechanism of yuzu focusing on ADP granule secretion, $TXB_2$ formations, and $PLC{\gamma}$/Akt signaling. The results from this study showed that ethanolic yuzu extract (YE), and its components, hesperidin and naringin, inhibited human platelet aggregation in a concentration-dependent manner. YE, hesperidin and naringin also inhibited $TXB_2$ formation and ADP release. The phosphorylation of $PLC{\gamma}$ and Akt was significantly inhibited by YE, heperidin and naringin. Furthermore, we demonstrated that YE, heperidin and naringin has anti-platelet effects in rat ex vivo studies, and lower side effects in mice tail bleeding time studies. The results from this study suggest that YE, hesperidin and naringin can inhibit human platelet aggregation, at least partly through the inhibition of $PLC{\gamma}$ and Akt, leading to a decrease in $TXB_2$ formation and granule secretion.

Screening of Antioxidative, Anti-platelet Aggregation and Anti-thrombotic Effects of Clove Extracts (정향 추출물의 항산화.항혈소판 응집효과 및 혈전 용해능 탐색)

  • Yang, Young-Yi;Lee, Min-Ja;Lee, Hye-Sook;Park, Won-Hwan
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.3
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    • pp.471-481
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    • 2011
  • Clove has been frequently used as anti-diabetic, anti-microbial, anti-inflammatory, anesthetic drug and remedies for stomachache by coldness. In this study, the antioxidant activity of extract from Clove was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation. Anti-platelet aggregation and anti-thrombotic effects of Clove extracts were studied ex vivo methods by mesuring the inhibitory effect on thrombin induced platelet aggregation and the fibrinolytic activity. The Clove extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation in vitro. Moreover Clove extracts were exhibited remarkable inhibitory effect on platelet aggregation and fibrinolytic activity. In conclusion, the Clove extracts have anti-oxidative and anti-atherosclerotic effects in vitro and ex vivo system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

Platelet Anti-Aggregation of Paeony Root

  • Kang, Sam-Sik;Kim, Ju-Sun;Kim, Eun-Mi;Yun-Choi, Hye-Sook
    • Korean Journal of Pharmacognosy
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    • v.22 no.4
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    • pp.215-218
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    • 1991
  • Paeony root was extracted and fractionated for the identification of the platelet anti-aggregatory components. And gallic acid methylester was isolated as the major component from the active sub-fraction F302.

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A Trend of Yin-tonifying Formulas Compared with Yang-tonifying Formulas on Anti-platelet and Anti-thrombotic Activity

  • Jeon, Won-Kyung;Yoo, Bo-Kyung;Ahn, Sang-Young;Lee, Ju-Hyun;Ahn, Sang-Woo;Ko, Byoung-Seob
    • The Journal of Korean Medicine
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    • v.30 no.6
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    • pp.1-8
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    • 2009
  • Objectives: Formulas for treatment of yin or yang deficiency conditions have been commonly used in traditional Korean medicine. The aim of this study is to examine the possible inhibitory effects of yin- or yang-tonifying formulas on in vivo anti-platelet activity and in vivo anti-thrombotic activity. Methods: We tested the effects of 26 types of yin- or yang-tonifying formulas on platelet aggregation induced by collagen in human whole blood using the impedance method of aggregometry and accessed a biomarker of platelet activation using thromboxane $B_2$ immunoassay. We also tested the anti-thrombotic effects of effective candidates on experimental models of thrombosis in mice. Results: 3 types of yin-tonifying formulas and 3 types of yin-yang-tonifying formulas were selected to be the most effective candidates (p<0.01). Also, through in vivo study, the antithrombotic activities of Igyeong-tang, Gamisipjeondaebo-tang, and Gamisoyo-san-treated groups, with recovery rate of 60, 50, and 45.45%, respectively, were observed to be higher than those of the control group (saline, 36.8%) in mouse acute thrombosis. Conclusion: These results show that yin-tonifying formulas are more effective in anti-platelet and anti-thrombotic activity than yang-tonifying formulas.

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