• 대한화장품학회지
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• 제42권3호
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• pp.285-295
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• 2016

큰실말(Cladosiphon novae-caledoniae kylin, C. novae-caledoniae kylin) 부산물을 이용하여 화장품 소재로써 가능성을 조사하고자, 항산화 효과(DPPH 자유 라디칼 소거 활성), 항균(anti-microbial activity), 미백(tyrosinase inhibition assay), 주름개선(elastase inhibition assay) 및 B16F10 mouse melanoma cell (MTT assay)을 이용한 세포 생존률을 측정하였다. 큰실말 추출물 원물(MC)과 불용성(MI)은 Staphyloccus aureus (S. aureus)와 항생제내성균주인 MRSA에 대하여 활성을 나타내었지만 항곰팡이 활성은 나타내지 않았다. 큰실말 추출물 수용성(MS)은 tyrosine에 대한 tyrosinase 저해효과가 49%로 표준물질인 arbutin 보다 미백효과가 우수하였지만 주름개선효과는 비교적 낮은 활성을 나타내었다. DPPH 라디칼 소거능력은 $500{\mu}g/mL$에서 89%로 나타내었다. 따라서 본 연구인 큰실말 추출물은 화장품 미백소재로써 좋은 후보군임을 확인할 수 있었다.

• 한국축산식품학회지
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• 제34권1호
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• pp.26-32
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• 2014

This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN-${\gamma}$ caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN-${\gamma}$-induced inflammation, anti-collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.50-59
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• 2003

BMB-CF has been constituted of purified fractions of Scutellaria baicalensis, which has medicinal effect such as anti-microbial. anti-inflammation. fever remedy. anti-oxidation. and anti-aging effect etc.. It has been used in traditional medicine formula from long time ago in the east Asia. It is constituted of the active flavone ingredients such as baicalin. baicalein. DTF(Di-methyl Tetra -hydroxy Flavone), wogonin. wogonoside. $\beta$- Sitosterol. etc.. General purified fractions of Scutellaria baicalensis has the high portion of the baicalin which has the problem of narrow antimicrobial spectrum and compatibility against cosmetic formula. Now. we has been develop the new purificaton process of Scutellaria baicalensis that has the high rate of DTF content, which is improved in antimicrobial activity and cosmetics compatibility. So. we have assure that it is the potent preservative against various cosmetic formula.

• The Korean Journal of Physiology and Pharmacology
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• 제25권3호
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• pp.197-206
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• 2021

Carnosol is a phenolic diterpene phytochemical found in rosemary and sage with reported anti-microbial, anti-oxidant, anti-inflammatory, and anti-carcinogenic activities. This study aimed to investigate the effect of carnosol on the lineage commitment of mouse bone marrow-derived mesenchymal stem cells (mBMSCs) into osteoblasts and adipocytes. Interestingly, carnosol stimulated the early commitment of mBMSCs into osteoblasts in dose-dependent manner as demonstrated by increased levels of alkaline phosphatase activity and Alizarin red staining for matrix mineralization. On the other hand, carnosol significantly suppressed adipogenesis of mBMSCs and downregulated both early and late markers of adipogenesis. Carnosol showed to induce osteogenesis in a mechanism mediated by activating BMP signaling pathway and subsequently upregulating the expression of BMPs downstream osteogenic target genes. In this context, treatment of mBMSCs with LDN-193189, BMPR1 selective inhibitor showed to abolish the stimulatory effect of carnosol on BMP2-induced osteogenesis. In conclusion, our data identified carnosol as a novel osteoanabolic phytochemical that can promote the differentiation of mBMSCs into osteoblasts versus adipocytes by activating BMP-signaling.

• 한국응용과학기술학회지
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• 제33권2호
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• pp.333-341
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• 2016

산수유 종자 추출물의 천연 방부제로서 가능성을 평가하기 위하여 항균활성 및 화장품 방부 효능 평가를 실시하였다. 산수유 종자는 각각 1,3-부틸렌글리콜과 에탄올을 이용하여 추출하였다. Paper disc method로 항균활성을 평가한 결과 Staphylococcus aureus, Staphylococcus epidermidis와 Propionibacterium acnes에서 항균활성이 나타났으며 산수유 종자 1,3-부틸렌글리콜 추출물(COS-A)이 여드름균에대해 $26.7{\pm}4.0mm$로 가장 높았다. 최소저해농도(MIC) 측정 결과 산수유 종자 에탄올 추출물(COS-B)이 가장 낮은 농도에서 S. epidermidis균의 생장을 억제하였다. 에멀션에서의 방부효능 평가를 위해 Challenge test를 실시한 결과 COS-A와 COS-B를 첨가한 에멀션에서 균 접종 7일 후 피부상재균이 100% 사멸되었다. 이러한 결과는 산수유 종자 추출물이 화장품에서 합성방부제를 대체할 천연 방부제로서 가능성이 있을 것으로 사료된다.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.53-79
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• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth promoting effect was measured using hair growth index. As a result, Prunus mume, black bean, Brassica campestris subsp. black sesame and Rubi Fructus showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Prunus mume, Eriobotryae Folium showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of the effect of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT - PCR analyses were performed. However, there were no plant extracts, which have profound effect on the gene expression of several growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another tests for inhibition of microbial such as P. acne were also carried out to find whether these plant extracts have anti -microbial activities. Rubi Fructus showed anti -microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제24권4호
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• pp.337-345
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• 2021

The immune system is not fully developed in human neonates and infants; breastfeeding is important in this stage as the bioactive components of human breast milk are known to have anti-microbial, anti-inflammatory, and immunomodulatory effects, and can therefore contribute to an infant's immunity against allergies, asthma, autoimmune diseases, and inflammatory bowel disease. Herein, the positive effect on the immune system by human colostrum and milk are reviewed.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.131-134
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• 2006

The in vitro anti-fungal activity of hydroxylated fatty acids obtained from microbial conversion by Psuedomonas aeruginosa PR3 using ricinoleic acid(RA), eicosadienoic acid(EDA) and conjugated linoleic acid(CLA) as substrates, was investigated. Bioconverted hydroxylated fatty acids showed different anti-fungal activities potentials against the range of phytopathogenic fungi such as Botrytis cinerea, Rhizoctonia solani, Fusarium oxysporum, Sclerotonia sclerotiorum, Colletotricum capsici, Fusarium solani and Phytophthora capsici. RA and EDA showed up to 50% fungal mycelial inhibition at the concentration of $5{\mu}l\;ml^{-1}$. RA, EDA and CLA also exhibited anti-fungal activities with minimum inhibitory concentration(MIC), ranging from 500 to $1000{\mu}g\;ml^{-1}$. Screening was also carried out using varied concentrations of bioconverted RA and EDA for determining the anti-fungal effect on the spore germination of different fungi. Bioconverted RA and EDA showed a considerable degree of spore germination inhibition.

• 동의생리병리학회지
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• 제25권3호
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• pp.471-481
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• 2011

Clove has been frequently used as anti-diabetic, anti-microbial, anti-inflammatory, anesthetic drug and remedies for stomachache by coldness. In this study, the antioxidant activity of extract from Clove was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$-induced human LDL oxidation. Anti-platelet aggregation and anti-thrombotic effects of Clove extracts were studied ex vivo methods by mesuring the inhibitory effect on thrombin induced platelet aggregation and the fibrinolytic activity. The Clove extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation in vitro. Moreover Clove extracts were exhibited remarkable inhibitory effect on platelet aggregation and fibrinolytic activity. In conclusion, the Clove extracts have anti-oxidative and anti-atherosclerotic effects in vitro and ex vivo system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• 대한소아치과학회지
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• 제36권2호
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• pp.237-244
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• 2009

치근단 감염 시 물리적인 작용으로 증상이 완화되지 않을 때, 근관 내 세균을 제거하기 위해 항균성을 지닌 소독제나 세척제가 필요하다. 천연항균제에 대한 관심이 증가하면서 여러 연구들에 의해 고추냉이 추출물의 구성 성분 중 하나인 allyl isothiocyanate에 대한 항균성이 밝혀졌다. 이에 본 연구에서는 근관내 감염시 자주 발견되는 세균들 중 편성혐기성 세균인 Fusobacterium nucleatum, Prevotella nigrescence, 그리고 항생제에 내성이 커서 항균 작용실험 시 대조균으로 자주 사용되는 Clostidial perfringens에 대한 서양산 고추냉이 뿌리 추출물의 항균효과를 알아보고, 대표적인 항균제인 클로르헥시딘과 비교하여 다음과 같은 결과를 얻었다. 1. 본 연구에 사용된 3종의 편성 혐기성 세균에 대한 서양산 고추냉이 뿌리 추출물의 최소 억제 농도는 평균 87-470 ppm, 최소 살균 농도는 평균 156-625 ppm으로 F. nucleatum에 대해 가장 강한 항균효과를 나타내었으며, 항균제에 저항성 이 큰 C. perfringens에도 항균효과를 나타내었다. 2. 본 연구에 사용된 3종의 편성 혐기성 세균에 대한 클로르헥시딘의 최소억제 농도는 평균 3.12-6.25 ppm, 최소 살균 농도는 평균 10.94 ppm이었다. 3. 서양산 고추냉이 뿌리 추출물은 87-470 ppm의 농도에서 3.12-6.25 ppm의 클로르헥시딘과 비슷한 세균성장 억제 효과를 가지며 156-625 ppm의 서양산 고추냉이 뿌리 추출물은 10.94 ppm의 클로르헥시딘과 비슷한 살균 효과를 보였다.