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http://dx.doi.org/10.5851/kosfa.2014.34.1.26

Effects of Egg Shell Membrane Hydrolysates on Anti-Inflammatory, Anti-Wrinkle, Anti-Microbial Activity and Moisture-Protection  

Yoo, Jinhee (Department of Food Science and Biotechnology, Sungkyunkwan University)
Park, Kimoon (Department of Food Science and Biotechnology, Sungkyunkwan University)
Yoo, Youngji (Department of Food Science and Biotechnology, Sungkyunkwan University)
Kim, Jongkeun (Sungkyun Biotech Co., LTD. R&D Center)
Yang, Heejin (Sungkyun Biotech Co., LTD. R&D Center)
Shin, Youngjae (Department of Environmental Horticulture, Dankook University)
Publication Information
Food Science of Animal Resources / v.34, no.1, 2014 , pp. 26-32 More about this Journal
Abstract
This study was conducted to examine the effects of eggshell membrane hydrolysates (ESMH) on the anti-inflammatory, anti-wrinkle, anti-microbial activity, and moisture-protection for cosmetic use. Whole ESMH (before fractionation), and fraction I (>10 kDa), fraction II (3-10 kDa), and fraction III (<3 kDa) of the hydrolysates were assessed in this experiment. As lipopolysaccharide (LPS) and IFN-${\gamma}$ caused the inflammation on Raw264.7 cell, whole ESMH and fraction I showed to be effective in inhibiting the induction of cell inflammation depending on the concentration, and also showed outstanding effect to suppress the skin inflammation. Fraction I inhibited collagenase and elastase activities to a greater extent than the other fractions, while all fractions had antibiotic effects at concentrations of 10 mg/disc and 20 mg/disc. In addition, it showed the moisture protection effects of skin on the holding amount and losing amount of moisture in upper-inner arm of the human body with a relatively low loss rate in skin, which confirmed that the hydrolyzed fractions of ESM helps to form the superior protective layer of moisture. It was concluded that ESMH fractions with different molecular weights, especially the 10 kDa fraction, have anti-lipopolysaccharide, anti-IFN-${\gamma}$-induced inflammation, anti-collagenase and elastase activities, and thus can be used as a cosmetic agent to protect skin.
Keywords
eggshell membrane; anti-inflammation; anti-aging; antibiotic; moisture protection;
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