• Natural Product Sciences
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• 제8권2호
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• pp.58-61
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• 2002

Five coumarins and a pyrimidine were isolated from the roots of Angelica gigas. Their structures were elucidated as bergapten (1), decursinol angelate (2), decursin (3), nodakenetin (4), uracil (5) and nodakenin (6) by spectral analysis. Among them, bergapten (1) and uracil (5) were isolated for the first time from this plant part.

• 약학회지
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• 제44권2호
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• pp.119-127
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• 2000

This paper describes isolation and elucidation structure of the components from leaves and evaluate the radical scavenging activity on DPPH radical for antioxidant effect. Bioassay guided fractionation of MeOH extract afforded active EtOAc and BuOH factions. The most active EtOAc fraction was repeatedly chrormatographed over silica and Sephadex LH-20 to afford six flavonoid compounds. Studies on the antioxidant activity of these constituents showed that quercetin was the most active of these compounds. Luteolin and kaempherol are also active, These results suggested that the antioxidant activity of leaves of Angelica gigas may be due to flavonoid components. All the compounds were identified by spectroscopic methods and are the first report from leaves of Angelica gigas.

• 생약학회지
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• 제27권3호
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• pp.254-261
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• 1996

A potent immuno-stimulating activity was detected from the watersoluble and ethanol-precipitated crude extract (AG-1) of the root of Angelica gigas Nakai. The crude extract was fractionated into two fractions, an acidic AG-2 and a neutral AG-3 fraction by DEAE-cellulose adsorption. The two fractions contained polysaccharides of which M.W. were 10 Kdal and >2,000 Kdal respectively, proteins, and various inorganic components. The immunostimulating activities of two fractions were not reduced by proteinase K, acid or alkali treatment. The polysaccharides obtained from the root of A. gigas were mainly composed of arabinose, galactose, and galacturonic acid. These results indicated that immuno- stimulating components of the root of A. gigas was a kind of pectic polysaccharides or arabinogalactans.

• 생약학회지
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• 제42권2호
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• pp.103-106
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• 2011

Angelica species from Korea has been used mainly as to promoting blood circulation, relieving pain and headache in common cold in Korea. To clarify the botanical origin of Angelica species from Korea, the anatomical characteristics of Angelica gigas, Angelica koreanum and Angelica purpuraefolia were studied. As a result, it was found the fact that the internal morphological patterns for the three Angelica species could discriminate from them by the secretory canal, xylem fiber and vascular bundle. According to these three criteria, Angelica species from Korea could be clearly distinguished so that the toxication by Angelica purpuraefolia can be prevented.

• 약학회지
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• 제56권3호
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• pp.152-157
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• 2012

Angelica gigas is one of the most widely used herbal medicines in Asia. Root extract of Angelica gigas is known to have anti-oxidant activity and skin whitening effect. The aim of this study was to prepare microemulsion system of root extracts of Angelica gigas for topical delivery. Microemulsion was successfully prepared by using MCT (medium chain triglyceride) as an oil phase, Labrasol as a surfactant, and the mixture of propyleneglycol and phosphatidylcholine (4 : 1) as a cosurfactant. In vitro and in vivo skin permeation and deposition of decursin, as a marker, was determined using hairless mouse. Microemulsion significantly increased the in vitro skin permeation of decursin for up to 12 hours and was significantly higher than the control (water). Moreover, microemulsion formulation showed significantly higher skin deposition of decursin compared to the control in both in vitro and in vivo studies. Thus, microemulsion could be a useful vehicle for topical application of root extracts of Angelica gigas.

• 한국환경복원기술학회지
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• 제12권1호
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• pp.92-100
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• 2009

This study was carried out to investigate vegetation structure and soil properties of Angelica gigas population distributed in Jeombongsan, Bangtaesan, Odaesan, Gyebangsan and Jirisan. From August 2007 until September 2008, 5m${\times}$5m quadrat was established in native area of Angelica gigas in order to record a dominants and coverage, and soil factors at 20 sites. It was found that the altitude in the distributed areas for Angelica gigas population was 710m or more. Angelica gigas population was classified into Parasencio auriculata var. kamtschatical dominant population, Pseudostellaria palibiniana dominant population, Isodon excisus dominant population and typical dominant population. In the site of study, soil pH, electrical conductivity, soil organic matter, available phosphorous, and exchangeable potassium, exchangeable calcium, exchangeable magnesium, exchangeable natrium concentration and total nitrogen were ranged from 5.1~6.2, 0.1~0.79dS/m, 2.21~22.11%, 9.56~37.97mg/kg, 1.85~23.88cmol+/kg, 0.21~1.18cmol+/kg, 1.07~5.09cmol+/kg, 0.04~0.14cmol+/kg and 0.09~1.04% respectively. The Parasencio auriculata var. kamtschatical dominant population was found in area of high altitude and had high slope degree. But Pseudostellaria palibiniana dominant population was found in area of low altitude and low slope degree. Isodon excisus dominant population was found to have higher soil pH and exchangeable potassium concentration than the other dominant population.

• 한국약용작물학회지
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• 제26권5호
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• pp.417-429
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• 2018

Background: The objective of this study was to make colloidal dispersions of the active compounds of radix of Angelica gigas Nakai that could be charaterized as nano-composites using hot melt extrusion (HME). Food grade hydrophilic polymer matrices were used to disperse these compound in aqueous media. Methods and Results: Extrudate solid formulations (ESFs) mediated by various HPMCs (hydroxypropyl methylcelluloses) and Na-Alg polymers made from ultrafine powder of the radix of Angelica gigas Nakai were developed through a physical crosslink method (HME) using an ionization agent (treatment with acetic acid) and different food grade polymers [HPMCs, such as HP55, CN40H, AN6 and sodium alignate (Na-Alg)]. X-ray powder diffraction (XRD) analysis confirmed the amorphization of crystal compounds in the HP55-mediated extrudate solid formulation (HP55-ESF). Differential scanning calorimetry (DSC) analysis indicated a lower enthalpy (${\Delta}H=10.62J/g$) of glass transition temperature (Tg) in the HP55-ESF than in the other formulations. Infrared fourier transform spectroscopy (FT-IR) revealed that new functional groups were produced in the HP55-ESF. The content of phenolic compounds, flavonoid (including decursin and decursinol angelate) content, and antioxidant activity increased by 5, 10, and 2 times in the HP55-ESF, respectively. The production of water soluble (61.5%) nano-sized (323 nm) particles was achieved in the HP55-ESF. Conclusions: Nano-composites were developed herein utilizing melt-extruded solid dispersion technology, including food grade polymer enhanced nano dispersion (< 500 nm) of active compounds from the radix of Angelica gigas Nakai with enhanced solubility and bioavailability. These nano-composites of the radix of Angelica gigas Nakai can be developed and marketed as products with high therapeutic performance.

• 융합정보논문지
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• 제11권6호
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• pp.206-212
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• 2021

본 연구는 참당귀에서 유효 성분을 보다 효율적으로 추출하는 방법을 찾아 참당귀의 효능을 극대화하는 것을 목표로 하였다. 열수, 에탄올 및 초임계 이산화탄소 추출법으로 참당귀를 추출한 후, 데커신, 데커시놀 안젤레이트 함량 분석, 총 폴리페놀 함량 정량, 그리고 항산화, 미백, 항균에 대한 효능 평가를 진행하였다. 데커신, 데커시놀 안젤레이트의 함량은 초임계 이산화탄소 추출물에서 38.65%로 매우 높았고, 총 폴리페놀 함량은 열수 추출물, 에탄올 추출물, 초임계 이산화탄소 추출물 순으로 높았지만, 그 차이가 비교적 적었다. 항산화 효능은 총 폴리페놀 함량과 경향이 일치하였고, 항균 효능은 데커신, 데커시놀 안젤레이트 함량과 경향이 일치하였다. 본 연구를 통해 참당귀에서 유효 성분을 추출하기 위한 최적의 방법은 초임계 이산화탄소 추출법임을 확인하였다.

• Journal of Dairy Science and Biotechnology
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• 제37권4호
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• pp.237-246
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• 2019

참당귀잎 추출물을 일정비율로 첨가한 발효유를 제조하고, 이화학적 특성과 항산화능 및 관능평가 등 품질특성을 분석하였다. pH는 4.40-4.45 범위를 보였고, 산도는 0.96%-0.98%로 참당귀잎 추출물 첨가에 따른 유의적인 차이를 보이지 않았다. 점도는 참당귀잎 추출물의 첨가에 따라 다소 감소되는 경향을 보였지만, 저장중 안정성에는 영향을 주지 않았다. 유산균수는 참당귀잎 추출물의 첨가량 증가에 따라 다소 억제되는 경향을 보였지만, 0.1%-0.3%의 범위에서는 1.9×10⁹-3.2×10⁹ CFU/mL의 높은 수준을 보였다. 요구르트 중의 decursin 함량 분석은 0.1%-0.3%의 첨가량에 따라 0.26 ㎍/g, 15.23 ㎍/g 및 23.57 ㎍/g으로 비례하여 정량분석 되었다. 유기산 생성은 lactic acid의 생성량이 가장 높게 나타났으며, 첨가비율에 따른 유의성은 보이지 않았다. 요구르트의 항산화능은 참당귀잎 추출물의 첨가로 높게 나타났으며, 또한 첨가량이 증가할수록 항산화효과도 높게 나타나는 효과를 보였다. 관능평가 결과, 참당귀잎 추출물 0.1% 첨가구가 무첨가구와 유사한 수준으로 높게 평가되었으며, 0.2% 첨가구의 경우도 6.31-6.50의 보통이상 수준으로 평가되었다. 이상의 결과와 같이 참당귀잎 추출물의 첨가농도는 0.2% 이내 범위가 적합하였다.

• 생명과학회지
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• 제20권9호
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• pp.1385-1393
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• 2010

국내산 참당귀의 생리활성 물질을 이용한 건강 기능성 식품 또는 화장품 소재 개발을 위한 기초연구의 일환으로 참당귀 뿌리를 3종류의 곰팡이 균주, A. oryzae, A. kawachii 및 M. purpureus 균주로 발효시켜 polyphenolic compounds, flavonoids, minerals, decursin 및 decursinol angelate 함량 분석과 항산화, 환원력 및 tyrosinase 활성을 측정 하였다. 참당귀 뿌리의 decursin과 decursinol angelate의 총 면적에 대한 함량 비율은 각각 44.0 및 27.3% 였으며, 한편 A. oryzae, A. kawachii 및 M. purpureus에 의한 발효 당귀의 decursin 함량 비율은 각각 52.0, 54.4 및 53.7% 였다. 참당귀 뿌리의 polyphenolic compounds 함량은 2.78% 였으나 발효 당귀에서 모두 약간씩 감소하였고, flavonoid 함량은 1.03% 함유되었으나 M. purpureus 발효 당귀에서 1.18%로 약간 증가하였다. 참당귀 뿌리의 미네랄 조성은 K, Mg, Fe, Ca, Na, Mn, Zn 순이였으며, 발효당귀에서 Na은 감소하고 나머지는 모두 증가하였다. 참당귀 뿌리의 항산화 활성 19%에 비해 A. oryzae, A. kawachii 및 M. purpureus 발효 당귀에서 각각 46, 49 및 56%로 모두 2배 이상 증가하였다. Fe- 및 Cu-환원력은 참당귀 뿌리보다 발효 당귀에서 모두 낮아지는 경향을 보였다. Tyrosinase 저해 활성은 당귀 뿌리의 18%에 비해 A. oryzae, A. kawachii 및 M. purpureus 발효 당귀에서 각각 34, 37 및 45%로 발효에 의해 2배 이상 증가하였다. 이상의 실험결과 발효 당귀에서 대표적인 화합물인 decursin과 decursinol angelate 함량이 증가하고, 항산화 활성 및 tyrosinase 저해 활성이 증가됨으로서 이를 활용한 건강기능 제품 개발 가능성이 높아졌으며, 이러한 제품 개발에 필요한 기초 자료를 제공해주는데 유용하게 사용될 것이다.