• 제목/요약/키워드: Amino acid content

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국내 야생수집 버섯의 아미노산 함량 분석 (The amino acid contents of wild mushrooms in Korea)

  • 안기홍;조재한;한재구
    • 한국버섯학회지
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    • 제18권1호
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    • pp.107-114
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    • 2020
  • 본 연구는 국내에서 수집한 15종의 야생버섯류를 식용가능 및 약용가능, 식독불명 또는 독버섯으로 분류하여 아미노산 성분함량을 분석하였다. 식용가능한 야생버섯들의 총 아미노산 성분함량의 범위는 866.4 ~ 1,220.5 mg/kg이었으며, 필수 아미노산의 총 함량은 301.4 ~ 490.2 mg/kg이었다. 그 중 흰우단버섯(OK811)에서 다량의 류신(Leu), 페닐알라닌(Phe), 시스테인(Cys), 글루탐산(Glu) 함량을 보이며 식용가능한 야생버섯류 중에서 총 필수 아미노산 함량이 가장 높았다. 약용가능한 버섯류의 총 아미노산 성분함량의 범위는 802.6 ~ 1,233.7 mg/kg이었으며 필수 아미노산 총 함량의 범위는 263.5 ~ 412.8 mg/kg이었다. 그 중 간버섯속(OK1071)의 총 아미노산 및 필수 아미노산 함량이 약용가능한 야생버섯류 중에서 가장 높았다. 특히 비필수 아미노산 성분인 시스테인(Cys)은 204.1 mg/kg가 검출되어 약용가능 야생버섯 뿐만 아니라 식용가능 야생버섯류에 비하여 훨씬 높은 함량을 나타냈다. 독버섯 또는 식독불명 버섯류의 총 아미노산 함량 범위는 681.8 ~ 1223.4 mg/kg 이었으며, 필수 아미노산의 함량의 범위는 229.1 ~ 442.8 mg/kg이다. 독버섯으로 알려진 노랑다발(OK826)은 이들 버섯류 중에서 가장 높은 총 아미노산 함량을 보였으며, 알기닌(Arg)은 109.2 mg/kg로 식용 또는 약용가능한 버섯류들에 비하여 높게 검출되었고 페닐알라닌(Phe)은 120.6 mg/kg로 약용가능한 야생 버섯류들에 비하여 높게 나타났다. 본 연구결과는 향후 야생버섯을 이용한 신품종 또는 산업적 이용 등을 위한 우수 버섯자원 선발에 필요한 기초자료로 활용하고자 한다.

크릴(Euphausia superba)의 영양성분 및 아미노산 조성에 관한 연구 (Studies on the Nutritional Components and Amino Acid Compositions of Krill (Euphausia superba))

  • 김한수;김민아;;강동수;장성호;류재용;이춘식;이원기
    • 한국환경과학회지
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    • 제23권2호
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    • pp.165-170
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    • 2014
  • Despite abundant nutritions, krill is barely used for human consumption. The objective of this study was to investigate the nutritional component and amino acid composition of krill and further utilizes it for food resource. Proximate compositions of krill meal were consisted of crude protein 58.85%, ash 13.89%, crude fat 12.45%, carbohydrate 11.01% and moisture 3.80%. The amount of total amino acid was 54.74 g%, and the major amino acids were glutamic acid (7.71 g%), aspartic acid (6.20 g%), leucine (4.72 g%), lysine (4.41 g%), arginine (6.49 g%) and alanine (3.25 g%), respectively. Total essential amino acid content was 21.87%, and the major amino acids were leucine (4.72 g%), lysine (4.41 g%) and isoleucine (2.90 g%), respectively. Content of total free amino acid in krill meal was 20224.30 mg/kg, and the major free amino acids were taurine (4501 mg/kg), arginine (3130.60 mg/kg), proline (2302.90 mg/kg), alanine (2088.10 mg/kg), glycine (1606.40 mg/kg) and lysine (1197.40 mg/kg). Especially, taurine was the most abundant of all free amino acids. Thus, these data indicate that krill seems to be abundant protein source food.

설농탕 주재료의 가열시간별 성분변화에 관한 연구 (A Research on the Changes in Components of Sulnong Soup Stock with Heating Times)

  • 임희수;안명수;윤서석
    • 한국식품조리과학회지
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    • 제1권1호
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    • pp.8-17
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    • 1985
  • Followings are the obtained results from the experiment of changes in content of proximate composition, free amino acid and nucleotides in soup prepared from beef brisket, leg bone, tripe and small intestine according to the diverse heating times-3, 6, 12, 18, 24, 30 hrs. The content of moisture in each sample is decreased from 97∼99% in 8 hours heating to 95∼97% after heating 30 hours. On the contrary, the content of crude protein and crude fat are gradually increased in proportion to the length of heating times and it showed a rapid increasement when it was boiled 6∼12 hours long. We can extract the most protein from the soup stock of tripe among all samples ana the most crude fat from the leg bone. The contents of free amino acids is gradually increased in proportion to the length of heating times. Especially after being boiled longer than 18 hours it is increased obviously. In the soup stock prepared from the brisket, the lysine and alanine were contained the most. In leg bone soup stock, glutamic acid and histidine were extracted the most but bone soup stock, glutamic acid contents were decreased a little in longer heating. In the soup stock of tripe, glutamic acid which is contained very little in a raw material was extracted more as increasing times. In the soup stock of small intestine, lysine and glutamic acid were extracted the most. The least content in free amino acid from each sample was cystine which is sulphur-containing amino acid. These result suggest that, in order to get enough extraction of amino acid, crude fat, 18 hours heating is the most useful while 5’-IMP, which is the taste compound of meat, is extracted at 3 hours heating.

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메주의 형상에 따른 재래식 간장의 유리아미노산 (Free amino acids in traditional Soy sauce prepared from Meju under different Formations)

  • 서정숙;이택수
    • 한국식생활문화학회지
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    • 제7권4호
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    • pp.323-328
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    • 1992
  • 여름철에 제조한 재래식의 벽돌형 메주(A)구, 국균접종의 벽돌형 메주(B구)와 콩알형 메주(C구)로 하절기에 간장을 담금하고 숙성과정중의 유리아미노산을 분석한 결과는 다음과 같다. 숙성기간과 시험구에 따라 다소 차이는 있으나 담금직후에 lysine, histidine, serine, glycine, valine, methionine, phenylalanine이, 90일에는 aspartic acid, glutamic acid, alanine, threonine, arginine, leucine 등이, 180일에는 isoleucine, tyrosine이 각각 검출되었다. Lysine과 histidine은 숙성과정 중 또는 시험구에서 증가 현상이 컸고, 검출된 유리아미노산 중 가장 함량이 높았다. Glutamic acid, aspartic acid, methionine 등은 숙성기간 중 감소하였다. 유리아미노산 총량은 숙성기간의 경과에 따라 증가되어 180일에 3,559-4,119mg/100ml으로 가장 높은 함량을 나타내었다. 국균접종의 콩알형 메주로 담금한 C구가 유리아미노산 총량이 가장 높았고 벽돌형 메주의 A구와 B구는 비슷하였다. 총질소나 아미노태질소는 벽돌형 메주로 담금한 간장이 높았다.

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김치 분말첨가 식빵의 품질 특성 (Quality Characteristics of White Pan Bread with Kimchi Powder)

  • 김래영;기미라;김문용;이군자;최현미;전순실
    • 동아시아식생활학회지
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    • 제15권3호
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    • pp.340-345
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    • 2005
  • White bread with 1, 2, 3, 4 and $5\%$ Kimchi powder added and control samples were tested for moisture content, volume, height, color, free amino acid content, texture, and sensory characteristics. Moisture content was about $40.46\~41.35\%$ and increased according to the addition of Kimchi powder. Volume and height were increased with increasing Kimchi powder content For the crust and crumb color, lightness was decreased, but yellowness and redness were increased as Kimchi powder content increased For free amino acid content, glutamic acid, alanine, serine and leucine were detected in the white bread, and samples with higher content of kimchi powder showed higher amount of total free amino acid content From the sensory analysis, one with $3\%$ kimchi powder got the highest score for flavor, texture, color, moistness and overall acceptability.

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Changes in Free Amino Acid, Carotenoid, and Proline Content in Chinese Cabbage (Brassica rapa subsp. Pekinensis) in Response to Drought Stress

  • Shawon, Rayhan Ahmed;Kang, Baek Song;Kim, Ho Cheol;Lee, Sang Gyu;Kim, Sung Kyeom;Lee, Hee Ju;Bae, Jong Hyang;Ku, Yang Gyu
    • 한국자원식물학회지
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    • 제31권6호
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    • pp.622-633
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    • 2018
  • Chinese cabbage grown during autumn season is confronted with drought conditions for a certain period, especially during the early growth stage. In this study, we investigated the effects of drought stress on plant growth characteristics, as well as free amino acid, carotenoid, and proline in Chinese cabbage. Chinese cabbage seeds (Bulam Plus) were germinated, and all the seedlings were transplanted into plastic containers (28 cm diameter ${\times}$ 22 cm high) containing a commercial growth medium. The soil water content was measured and maintained at 10% for the drought-stressed plants and at 30% for the control plants, for three weeks. The results revealed that plant growth parameters were lower in the drought-stressed plants than in the control plants. The total free amino acid content tended to decrease in both drought-stressed and control plants with time. The total free amino acid content was found to be lower in the drought-stressed plants than in the control plants and the proline content was unaffected. Moreover, at three weeks after treatment, carotenoid content in drought stressed plants was significantly higher than that in the untreated plants. We believe that our study makes a significant contribution to the literature because the effects of drought stress on plant growth parameters, free amino acid, carotenoids, and proline accumulation in autumn growing cultivar of Chinese cabbage have not been widely studied in Korea, and our study provides valuable information in this regard, as Chinese cabbage is consumed throughout the year in Korea.

싹튼 콩으로 제조한 메주의 발효기간에 따른 품질변화 (Changes in Quality Characteristics of Meju Made with Germinated Soybean during Fermentation)

  • 최웅규;김미향;이난희;정연신;황영현
    • 한국식품과학회지
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    • 제39권3호
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    • pp.304-308
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    • 2007
  • 본 연구에서는 24시간 동안 싹튼 콩을 원료로 하여 콩알 메주를 제조한 후 발효기간에 따른 각종 품질의 변화를 조사하였다. 발효 전 기간 동안 pH는 6.3-6.5로 큰 변화가 없는 것으로 조사되었다. 증자 직후의 아미노태 질소함량은 15.5 mg%였으며, 발효가 진행됨에 따라 급격히 증가하여 발효 48시간째에는 312.9 mg%를 나타내었다. 발효기간별 미생물의 변화를 확인한 결과 곰팡이의 수가 높게 나타난 반면 호기성 세균과 효모의 수는 낮게 나타났는데 이는 메주의 제조 시 Aspergillus oryzae를 접종하였기 때문인 것으로 판단된다. 발효가 진행됨에 따라 유기산 함량은 점차 높아져 발효 48시간째에는 1,067.3 mg%가 검출되었으며 함량별로는 발효 전 기간에 걸처 citric acid가 가장 많이 검출되었다. 유리아미노산 함량은 발효가 진행됨에 따라 함량이 급격히 높아져 발효 48시간째에는 2,513.5 mg%로 발효 전에 비해 5.7배 가량 많이 검출되었다. 함량별로 보면 glutamic acid가 가장 많았으며, aspartic acid, lysine, leucine 및 proline의 순으로 많이 검출되었다. glutamic acid의 총아미노산에 대한 비율은 발효가 진행됨에 따라 점차 증가하여 발효 48시간째에는 21.2%로 나타났다. 총 Isoflavone의 함량은 발효초기에 123.9 mg%로 약간 증가한 후 그 수준을 지속적으로 유지하는 것으로 나타났으며, 발효 전 기간 동안 genistein>daidzein>glycitein의 순으로 나타났다.

韓國人蔘의 年根別 및 貯藏期間別 아미노酸分析 (A Study on the Analysis of Amino Acids in Korean Ginseng)

  • Rhee, Seong-Hong;Zong, Moon-Shik
    • 한국환경보건학회지
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    • 제9권2호
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    • pp.37-53
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    • 1983
  • The contents of amino acids were examined in the 3, 4, 5, and 6 year-old roots of fresh ginseng and the 1979, 1980, 1981, and 1982 years' products of white and red ginsengs. Samples extracted with 75% ethanol for free amino acids and hydrolyzed with 6N-HCL for total amino acids were analyzed by Amino Acid Analyzer (Hitachi model KLA-5). The results were summarized as follows: 1. Amino acids from extracted samples were 18 kinds of Tryptophan, Lysine, Histidine, Arginine, Aspartic acid, Threonine, Serine, Glutamic acid, Proline, Glycine, Alanine, Cystine, Valine, Methionine, Isoleucine, Leucine, Tyrosine, and Phenylalanine. 2. Amino acids detected in hydrolyzed samples were 17 kinds execpt Tryptophan of extracted ones. 3. Arginine was the highest quantity of amino acids in ginseng. 4. The content of Tryptophan was 0.5690 mg/g in the 6 year-old fresh ginseng and trace quantities in other samples. 5. The contents of amino acids were increased in fresh ginseng according to cultivation year. 6. The contents of amino acids in white ginseng were slightly decreased but those in red ginseng were not changed during the storage time. 7. The content ratio of free amino acids to total amino acids were 1:3.

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일산화탄소 중독이 뇌내 아미노산 신경전달물질 함량변화에 미치는 영향 (The Effect of Carbon Monoxide Intoxication on the Changes in Contents of Amino Acid Neurotransmitter of Rat Brain)

  • 정민정;박송자;이선희;윤재순
    • 약학회지
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    • 제34권5호
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    • pp.323-333
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    • 1990
  • To study influence of carbonmonoxide (CO) poisoning on the content of amino acid neurotransmitter in brain, male rat was exposed to CO 5000 ppm for 30 minutes (60-75% HbCO). Aspartic acid and glutamic acid level in the cerebral cortex and aspartic acid level in the striatum were significantly decreased. GABA level in the cerebral cortex was significantly increased after the 30 and 60 minutes of CO intoxication. Taurine level in both the cerebral cortex and the striatum was increased although nonsignificant. Consequently, the CO-induced hypoxia brain showed lower level of excitatory neurotransmitter, aspartic acid and glutamic acid and higher level of inhibitory neurotransmitter, GABA and taurine. These results suggest that the change in content of amino acid neurotransmitter in the rat brain may be concerned with several CO poisoning symptoms.

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비중선별(比重選別) 현미중(玄米中) 유리 Amino산 함량 (Free amino acids of brown rice in relation to specific gravity grade)

  • 박훈;전재근;조인호
    • Applied Biological Chemistry
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    • 제15권1호
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    • pp.35-40
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    • 1972
  • The contents of free amino acids in deembryod brown rice of two varieties were investigated by amino acid autoanalizer in relation to specific gravity grade. The analytical methods of free amino acid were also discussed. 1) The lower the specific gravity of the unhulled rice the higher the content of total free amino acids in the deembryod brown rice, and the similar trend appears to hold on each amino acids. 2) Main free amino acids were serine+asparagine, glutamic acid, aspartic acid, alanine and valine, and maximum values of them were 7.3, 5.1, 4.0, 3.4, 0.9mg/100g rice, respectively. They consist about 85% of total free amino acids in most cases. 3) The contents of soluble nitrogen and free amino acids appear to be lower in high protein variety (IR 667) than in low protein variety (Jinhung). The percentage of free amino acid nitrogen to soluble nitrogen, however, appears to be higher in high protein variety (IR 667). 4) Alanine was much lower than aspartic acid in IR 667 having Indica blood while alanine appears to be higher than aspartic acid in Jinhung (Japonica rice) suggesting varietal difference in amino acid metabolism. 5) Threonine peak was overlaped with glutamine, and serine was with asparagine in this study.

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