• Title/Summary/Keyword: Alpine

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Quantitative Analysis of Flavonoids in the Unripe and Ripe Fruits and the Leaves of Four Korean Rubus species (한국산 4종 Rubus속 숙과, 미숙과 및 잎의 플라보노이드 함량 분석)

  • Kim, Min-Young;Choi, Moo-Young;Nam, Jung-Hwan;Park, Hee-Juhn
    • Korean Journal of Pharmacognosy
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    • v.39 no.2
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    • pp.123-126
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    • 2008
  • The five flavonoids, isoqueritrin, juglanin, astragalin and 2-O-trans-p-coumaroyl astragalin were quantitatively analyzed in the leaves, unripe- and ripe fruits of four Korean Rubus species including Rubus crataegifolius, R. pungens var. oldhami, R. parvifolius, and R. coreanus belonging to the Rosaceae family. The HPLC analysis demonstrated that flavonoids are more abundant in the leaves than in the fruits. In the four Rubus species, ripe fruits generally contained similar or more amount of flavonoids than in unripe fruits. In particular, the quantity levels of total flavonoid (49.81${\pm}$0.50 mg/g) and isoquercitrin (35.08${\pm}$0.37 mg/g) were highest in the leaf of R. crataegifolius. In addition, the content of the flavonoids were quite low in the unripe fruits of R. coreanus that is most widely used as Rubi Fructus.

Three Viruses Isolated form Gentiana spp. in Korea (용담(Gentiana spp.)에서 분리한 3종의 바이러스)

  • 노성환;장무웅
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.425-432
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    • 1998
  • Gentian plants (Gentiana spp.) showing yellow ringspot, mosaic, necrotic fleck and malformation were collected from their growing areas in Taegu, Kyungpook province and Alpine Agricultural Experiment Station, Korea. Three viruses isolated from the naturally infected gentian were identified as broad bean wilt virus (BBWV), cucumber mosaic virus (CMV) and clover yellow vein virus identified as broad bean wilt virus (CIYVV) by their host range, immunosorbent electron microscopy (ISEM) and electron microscopy. Electron microscopic examination of negatively stained preparations showed that BBWV and CMV are spherical particles of 28 nm and 30 nm in diameter, and CIYVV is filamentous particles of ca. 780 nm in length. By ISEM, BBWV was detected mainly in gentian showing yellow ringspot and mottle, CIYVV in necrotic fleck leaf of gentian and CMV in narrow and distorted mosaic leaf of gentian. BBWV and CMV are the most prevalent in the cultivated gentian. In ultrathin sections of BBWV infected tissues, large aggregates of crystalline array of virus particles and vesicular body were found in the cytoplasm and vacuole of mesophyll cells. In case of CIYVV, pinwheel- and laminated aggregate-type inclusions as well as filamentous virus particles were observed in the cytoplasm of mesophyll cells.

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Changes in Frequencies and Distribution of A2 Mating Type and Metalaxyl-Resistant Isolates of Phytophthora infestans in Korea (우라나라 감자 역병균 A2 교배형 및 Metalaxyl 저항성균의 빈도 및 분포의 변화)

  • ;;W. E. Fry
    • Korean Journal Plant Pathology
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    • v.10 no.2
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    • pp.92-98
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    • 1994
  • Phytophthora infestans populations collected from various geographical locations of Korea in 1991 and 1993 were analyzed for mating types and responses to metalaxyl. Both A1 and A2 mating type isolates were detected in 1991. The majority of the isolates were A2 mating type, but no A1 mating type was detected in 1993. About 40% of the isolates collected in 1991 were resistant to metalaxyl, and the distribution of metalaxyl-resistant isolates of P. infestans was strongly associated with their geographic origins in Korea. Metalaxyl-resistant isolates with EC50 values > 50$\mu\textrm{g}$/ml were collected from the northern provinces of Kangwon, Kyungbuk, and Chonbuk, but not from the southern provinces of Kyungnam, Chonnam, and Jeju in 1991. The drastic increase in the degree of quantitative resistance to metalaxyl was detected among the isolates from the southern provinces during 1991~1993. More than 50% of the isolates collected from the southern provinces of Kyungnam and Chonnam in 1993 had EC50 values >50$\mu\textrm{g}$/ml. The province of Kangwon had isolates with the greatest resistance to metalaxyl. this alpine areas might be the origin of metalaxyl-resistant isolates of P. infestans in Korea. The A2 genotype with metalaxyl resistance appears to be displacing the A1 genotype which is presently the predominant genotype in Korea.

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Detection of Colletotrichum acutatum and C. gloeosporioides by Real Time PCR (Real Time PCR을 이용한 Colletotrichum acutatum과 C. gloeosporioides의 검출)

  • Kim, Seung-Han;Kwon, Oh-Hun
    • Research in Plant Disease
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    • v.14 no.3
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    • pp.219-222
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    • 2008
  • Real time PCR was used to discriminate Colletotrichum acutatum and C. gloeosporioides for analysis of population density. Two primers, caInt2 and cgint, used for conventional PCR to discriminate two species were modified with fluorescent dye to make probe for real time PCR. Fluorescence signals were successfully detected by fCaInt2 and vCgint probe coupled with primer pair Unicon and Unicor1 resulting in discrimination of C. acutatum and C. gloeosporioides by comparison of delta Rn value.

A Study on Changes and Distributions of Korean Fir in Sub-Alpine Zone (아고산 지역의 구상나무 분포 변화에 관한 연구)

  • Kim, Nam-Shin;Lee, Hee-Cheon
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.16 no.5
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    • pp.49-57
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    • 2013
  • This study was carried out to analysis change of distribution and habitat location environment of Korean Fir which is typically vulnerable species by the climate change in Mt. Jiri and Halla. Korean Fir was decreased 18% during 27 year since year 1981, Mt. Halla was 34% during 15 years since year 1988. In the same periods, Temperature change was increased from 8.56 to $9.36^{\circ}C$, from 11.2 to $12.1^{\circ}C$. Distribution changes by the elevation showed higher change ratio 1,400~1,600m in Mt. Jiri and 1,200~1,900m in Mt. Halla. Changes of Korean Fir each slope aspects was high $180{\sim}360^{\circ}$ in Mt. Jiri, $45^{\circ}$ in Mt. Halla. In slope was $30^{\circ}$ in Mt. Jiri and $20^{\circ}$ in Mt. Halla. Changes by reliefs was 12 in Mt. Jiri, 0 or 15 in Mt. Halla, and Sites of Korean Fir was convex slopes both of two areas. Changes by soils was in the good drainage textures.

Preharvest Drop Control of 'Tsugaru' Apple with Combined Use of Aminoethoxyvinylglycine and Several Adhesive Agents (Aminoethoxyvinylglycine과 몇몇 전착제의 혼용처리에 의한 '쓰가루' 사과의 수확전 낙과방지)

  • Kang, Chung Kil;Chae, Yun Seok;Kim, Su-Jeong
    • Horticultural Science & Technology
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    • v.17 no.6
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    • pp.750-752
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    • 1999
  • This experiment was conducted to evaluate preharvest drop control in 'Tsugaru' apple with combined treatments of aminoethoxyvinylglycine (AVG) and several adhesive agents. AVG+Silwet L-77 at $75+1,000mg{\cdot}L^{-1}$ was most effective among the tested combinations. Little or no significant difference was observed among the different application times of AVG+Silwet L-77. AVG+Silwet L-77 significantly reduced preharvest drop while increasing fruit weight, fruit length, diameter and fruit firmness. All the tested combinations were generally effective in reducing preharvest drop as compared to dichlorprop and in maintaining better fruit quality.

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Constituents Analysis of Amino Acid and Antioxidative Activity from Cultivated Callus and Rhizome in Rhodiola sachalinensis

  • Song, Won-Seob;Chi, Hyung-Joon;Rim, Yo-Sup;Yoon, Jae-Ho
    • Plant Resources
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    • v.5 no.1
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    • pp.78-85
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    • 2002
  • The material of Rhodiola sachatinensis collected from an alpine region of the west-northern China. For analysing the effect, 1 used Rhodiola sachatinensis's rhizome and cultivated callus. In EtOAc, BuOH, $H_2O$ separation the plant showed strong antioxidative activity, but not in Hexane. The radical scavenging effect of EtOAc(RC$_{50}$,35(g), BuOH(RC$_{50}$, 43(g), H$_2$0(RC$_{50}$, 50(g) fraction and MeOH extract(RC$_{50}$, 50(g) of the Rhodiola sachatinensis was comparable to that of synthetic antioxidant BHA(RC$_{50}$, 14(g) and $\alpha$-Tocopherol(RC$_{50}$, 12(g). Total amino acid concentration of plant of In nature condition were 18,009ppm, and major components were arginine, glutamic acid, aspartic acid and valine. The ratio of essential/total amino acid on plant of In nature condition was 46.93%. Total amino acid concentration of callus of In vitro condition were 32,435ppm, and major components were valine, histidine, lysine and leucine. The ratio of essential/total amino acid on callus of In vitro condition was 56.07%. was 56.07%.

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Identification of Molecular Markers for Population Diagnosis of Korean Fir (Abies koreana) Vulnerable to Climate Change

  • Kim, Dong Wook;Park, Da Young;Jeong, Dae Young;Park, Hyeong Cheol
    • Proceedings of the National Institute of Ecology of the Republic of Korea
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    • v.1 no.1
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    • pp.68-73
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    • 2020
  • Korean fir (Abies koreana) is an evergreen coniferous tree species that is unique to South Korea. A. koreana is found in a limited sub-alpine habitat and is considered particularly vulnerable to climate change. Identification of populations vulnerable to climate change is an important component of conservation programs. In this study, a heat stress-induced transcriptome RNA-seq dataset was used to identify a subset of six genes for assessment as candidate marker genes for ecologically vulnerable populations. Samples of A. koreana were isolated from ecologically stable and vulnerable regions of the Halla and Jiri mountains, and the expression levels of the six candidate markers were assessed using quantitative real-time polymerase chain reaction. All six of the candidate genes exhibited higher expression levels in samples from vulnerable regions compared with stable regions. These results confirm that the six high temperature-induced genes can be used as diagnostic markers for the identification of populations of A. koreana that are experiencing stress due to the effects of climate change.

Kinematic Comparative Analysis of Short Turns between Skilled and Unskilled Alpine Skiers

  • Jo, Hyun Dai
    • Korean Journal of Applied Biomechanics
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    • v.29 no.4
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    • pp.219-226
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    • 2019
  • Objective: The purpose of this study is to provide a better understanding of short turn mechanism by describing short turns after kinematic analysis and provide skiers and winter sports instructors with data through which they are able to analyze right postures for turns in skiing in a systematic, rational and scientific manner. Method: For this, a mean difference of kinematic variables (ski-hip angle, ski-shoulder twist angle, pole checking angle, the center of gravity (CG) displacement, trunk forward lean angle) was verified against a total of 12 skiers (skilled and unskilled, 6 persons each), regarding motions from the up-start to down-end points for short turns. Results: There was no difference in a ski-hip twist angle. The ski-shoulder twist angle was large at the up-start point while a pole-checking angle was high at the down-end point in skilled skiers. Concerning the horizontal displacement of CG, skilled skiers were positioned on the right side at the upstart point. No significant difference was observed in the trunk forward lean angle. Conclusion: According to the ski-shoulder twist angle and CG horizontal displacement results, the upper body should be kept leant toward the pole. In addition, big turns should be made via edging and angulation. During pole checking, the hand holding the pole should be thrown and released toward a vector direction of the forearm.

The Influence of the Landscape Meaning Illustrated to Subjects on their Landscape Preference (경관의미에 대한 정보전달이 경관선호에 미치는 영향)

  • 노재현
    • Journal of the Korean Institute of Landscape Architecture
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    • v.26 no.4
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    • pp.14-24
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    • 1999
  • The objective of this study is to test the influence of the landscape meaning illustrated to subjects on their landscape preference and to investigate the difference of the response(Influence Value;IV) between cultural landscape and natural landscape. For this, color-slide surrogates for cultural and natural landscape are shown to Experimental Group(EG) and Control Group(CG)(between-subject design). And Experimental Group is divided into meaning illustrated Before and After Group(within-subject design). For given 45 slide scenes of cultural and natural landscape, the subjects were asked to measure their preference on ten point scale. The results are as follows; 1. The influence of value illustration is positive in the preference. Specifically, this effect was more powerful for the low beauty landscape than for the high beauty landscape. 2. The relation between the IV and the preference of non illustrated group was deeply correlated. And IV of cultural landscape was higher than that of natural landscape. 3. It is identified that the meaning illustration about high naturalness landscape such as the swamp and alpine landscape is more effective than other natural landscape. 4. Also, the preference was more influenced by the personal task associated with landscape experience. But the statistical significance between the preference and experience about landscape didn't exist. Accordingly, it was suggested by this study that the illustrated meanings of landscape and perceived landscape form were also major influential factors on the landscape preference.

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