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http://dx.doi.org/10.5423/RPD.2008.14.3.219

Detection of Colletotrichum acutatum and C. gloeosporioides by Real Time PCR  

Kim, Seung-Han (Bonghwa Alpine Medicinal Plant Research Station)
Kwon, Oh-Hun (Yeongyang Pepper Research Station of Kyeongbuk Agricultural Research and Extension Service)
Publication Information
Research in Plant Disease / v.14, no.3, 2008 , pp. 219-222 More about this Journal
Abstract
Real time PCR was used to discriminate Colletotrichum acutatum and C. gloeosporioides for analysis of population density. Two primers, caInt2 and cgint, used for conventional PCR to discriminate two species were modified with fluorescent dye to make probe for real time PCR. Fluorescence signals were successfully detected by fCaInt2 and vCgint probe coupled with primer pair Unicon and Unicor1 resulting in discrimination of C. acutatum and C. gloeosporioides by comparison of delta Rn value.
Keywords
Colletotrichum acutatum; C. gloeosporioides; Detection of Colletotrichum spp; Real time PCR;
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