• Journal of Microbiology and Biotechnology
• /
• 제12권1호
• /
• pp.130-136
• /
• 2002

The internal transcribed spacer (ITS) region in the genus Tricholoma was analyzed, including for its primary nucleotide sequence and secondary structural characterization. The secondary structures of the ITS2 region in the genus Tricholoma were identified for use in bioinformatic processes to study molecular evolution and compare secondary structures. Ten newly sequenced ITS regions were added to the analysis and submitted to the GenBank database. The resulting structure from a minimum energy algorithm indicated the four-domain model, as previously suggested by others. The conserved secondary structure of the ITS2 sequences of the genus Tricholoma exhibited certain unique features, including pyrimidine tracts in the loops of domain A and a complete structure containing four domains, with motifs identified in other ITS2 secondary structures. A phylogenetic tree was derived from sequence alignment based on the secondary structures. From the resulting maximum parsimonious tree, it was found that the species in the genus Tricholoma had evolved monophyletically and were composed of four groups, as supported by the bootstrapping values and pileus color.

• 제어로봇시스템학회논문지
• /
• 제12권2호
• /
• pp.93-100
• /
• 2006

In this paper, a new-type Extended Kalman Filter (EKF) is proposed as a robust nonlinear filter for a stochastic nonlinear system. The original EKF is widely used for various nonlinear system applications. But it is fragile to its estimation errors because they give rise to linearization errors that affect the system mode1 as the modeling errors. The linearization errors are nonlinear functions of the estimation errors therefore it is very difficult to obtain the accurate error covariance of the EKF using the linear form. The inaccurately estimated error covariance hinders the EKF from being a sub-optimal estimator. The proposed filter tries to obtain the upper bound of the error covariance tolerating the uncertainty of the error covariance instead of trying to obtain the accurate one. It treats the linearization errors as uncertain modeling errors that can be handled by the robust linear filtering. In order to be more robust to the estimation errors than the original EKF, the proposed filter minimizes the upper bound like the robust linear filter that is applied to the linear model with uncertainty. The in-flight alignment problem of the inertial navigation system with GPS position measurements is a good example that the proposed robust filter is applicable to. The simulation results show the efficiency of the proposed filter in the robustness to initial estimation errors of the filter.

• 대한치과의사협회지
• /
• 제54권2호
• /
• pp.134-141
• /
• 2016

Use of ready-made orthodontic appliance can lead to inefficiencies in the final stages of the orthodontic treatment. Because patients' teeth have anatomic variations, brackets that have been designed to fit on average tooth surface may result in positional discrepancies when leveling and alignment is completed. As a result, additional steps such as rebonding, wire bending and use of auxiliaries may be needed. Even in patients who have normal tooth anatomy and proper tooth size relationships, precise bracket placement is crucial in order to efficiently control the tooth positions. Digital models can provide advantages in clinical orthodontics as virtual tooth setup could be performed, and clinicians can easily visualize the predicted final occlusion. Through this setup model, customized brackets with individualized prescription and archwires that optimally fit with the patients' dental arches can be produced using CAD/CAM technology. Also, the brackets can be accurately placed with an aid of 3D-printed jigs. The purpose of this article is to introduce the commonly used labial and lingual customized orthodontic appliance systems using digital technology.

• Fisheries and Aquatic Sciences
• /
• 제12권4호
• /
• pp.265-275
• /
• 2009

G Protein-coupled receptors (GPCRs) mediating wide ranges of physiological responses is one of the most attractive targets for drug development. Rhodopsin, a dim-light photoreceptor, has been extensively used as a model system for structural and functional study of GPCRs. Fish have rhodopsin finely-tuned to their habitats where the intensity and the wavelength of lights are changed depending on its water-depth. To study the detailed molecular characteristics of GPCR architecture and to understand the fishery light-sensing system, genes encoding rod opsins were isolated from fishes living under different photic environments. Full-length rod opsin genes were obtained by combination of PCR amplification and DNA walking strategy of genomic DNA isolated from olive flounder, P. olivaceus, Japanese eel, A. japonica, and Common carp C. carpio. Deduced amino acid sequences showed a typical feature of rod opsins including the sites for Schiffs base formation (Lys296) and its counter ion (Glu113), disulfide formation (Cys110 and Cys187), and palmitoylation (Cys322 and Cys323) although Cys322 is replaced by Phe in Japanese eel. Comparison of opsins by amino acid sequence alignment indicated the closest similarity between P. olivaceus and H. hippoglossus (94%), A. japonica and A. anguilla (98%), and C. carpio and C. auratus (95%), respectively.

• 한국공작기계학회논문집
• /
• 제17권6호
• /
• pp.118-123
• /
• 2008

Weakness process can be called ferrule array and epoxy filling process at connector manufacturing process, and a lot of problems happen as think as general manufacturing process at early investment. Wished to improve this and working environment mend of worker on childhood(planning phase) and problem that is happened at done ferrule array and epoxy injection by emphasis target. By ferrule sorting and Improvement of epoxy filling process, bring authoritativeness elevation of product by fraction defective decrease of product by sized work along with productivity elevation. On the other hand, working jigs are various in characteristics of optical connector manufacturing line. There have been lots of restriction in practice because the applicability of this system is only for single type model though the network should be smooth between lines. Thus, it is not only needed the recognition of necessity in industrial line but also the development of automation system arraying ferrule and filling epoxy in the manufacturing process. It is found that the present system development enhances productivity fairly and prevents industrial disaster in the optical connector manufacturing system.

• 반도체디스플레이기술학회지
• /
• 제9권1호
• /
• pp.17-21
• /
• 2010

Currently miniaturized electron-optical columns find their way into electron beam lithography systems. For better lithography process, it is required to make smaller spot size and longer working distance. But, the micro-columns of the multi-beam lithography system suffer from chromatic and spherical aberration, even when the electron beam is exactly on the symmetric axis of the micro-column. The off-axis error of the electron emitting source is expected to become worse with increasing off-axis distance of the focusing spot. Especially the electron beams far from the system optical axis have a non-negligible asymmetric intensity distribution in the micro-column. In this paper, the effect of the off-axis e-beam source is analyzed. To analyze this effect is to introduce a micro-column model of which the e-beam emitting source is aligned with the center of the electron beam by shifting them perpendicular to the system optical axis. The presented solution can be used to analysis the performance of the multi-electron-beam system. The performance parameters, such as the working distances and the focusing position are obtained by the computational simulations as a function of the off-axis distance of the emitting source.

• 항공우주기술
• /
• 제11권1호
• /
• pp.158-168
• /
• 2012

본 논문에서는 나로호 2단 킥모터 TVC 노즐에 대한 행정확인시험 방법과 행정확인시험 데이터로부터 TVC 운용 특성을 분석하는 과정 및 결과에 대해 다루었다. 개루프 행정확인 시험을 통해 TVC 스트로크와 포텐셔미터 전압 간의 관계를 분석하고, 폐루프 행정확인시험을 통해 노즐정렬오차, TVC 구동오차, TVC 중립위치, 플렉스씰 노즐의 회전중심위치, 비행시험후 분석에 사용할 노즐각 환산계수 등을 분석할 수 있음을 보였다. 아울러, 나로호 1호기 및 2호기 TVC 시스템에 대해 행정확인시험을 수행한 결과 TVC 운용 관련 모든 파라미터가 정상 범위 내에서 설정되었음을 정량적인 수치로써 제시하였다.

• Fisheries and Aquatic Sciences
• /
• 제13권4호
• /
• pp.263-271
• /
• 2010

G protein-coupled receptors (GPCR) constitute the largest superfamily of cell membrane receptors, mediating diverse signal-transduction pathways. The melanocortin 4 receptor (MC4R) has been of interest for its physiological role and size, one of the smallest among the GPCRs, which makes it a good model system for the structural study of GPCRs. To study the molecular structure and tissue-specific expression of MC4R in olive flounder (Paralichthys olivaceus), the full-length MC4R gene was obtained using PCR amplification of genomic DNA as well as cDNA synthesis. Sequence analysis of the gene indicates that 978 bp of the MC4R gene encodes 325 amino acids without introns. Sequence alignment with the MC4Rs from other fish shows the highest degree of identity (96%) between Paralichthys olivaceous and Verasper moseri, followed by Takifugu rubripes and Tetraodon nigroviridis (89%). RNA was isolated from various tissues to examine the tissue distribution of MC4R by using RT-PCR. The results showed major expression of MC4R in the liver, brain, and eye, which is consistent with the expression pattern in other fish belonging to the order Pleuronectiformes.

• Journal of Astronomy and Space Sciences
• /
• 제33권3호
• /
• pp.237-246
• /
• 2016

We analyze the design and specifications of the Sun-and-Stars Time-Determining group of instruments (Ilseong-jeongsi-ui, 日星定時儀) made during the Joseon dynasty. According to the records of the Sejong Sillok (Veritable Records of King Sejong), Sun-and-Stars Time-Determining Instruments measure the solar time of day and the sidereal time of night through three rings and an alidade. One such instrument, the Simplified Time-Determining Instrument (So-jeongsi-ui, 小定時儀), is made without the essential component for alignment with the celestial north pole. Among this group of instruments, only two bronze Hundred-Interval-Ring Sundials (Baekgak-hwan-Ilgu, 百刻環日晷) currently exist. A comparison of the functions of these two relics with two Time-Determining Instruments suggests that the Hundred-Interval-Ring Sundial is a Simplified Sundial (So-ilyeong, 小日影), as recorded in the Sejong Sillok and the Seongjong Sillok (Veritable Records of King Seongjong). Furthermore, the Simplified Sundial is a model derived from the Simplified Time-Determining Instrument. During the King Sejong reign, the Sun-and-Stars Time-Determining Instruments were used in military camps of the kingdom's frontiers, in royal ancestral rituals, and in royal astronomical observatories.

• Applied Microscopy
• /
• 제41권3호
• /
• pp.215-222
• /
• 2011

We discuss the experimental procedure for extracting reliable phase information from a defocus series of transmission electron microscopy (TEM) dark-field images using the transport of intensity equation (TIE). Taking InGaN/GaN multi-quantum well light-emitting diode as a model system, various factors affecting the final result of reconstructed phase such as TEM sample preparation, TEM imaging condition, image alignment, the correction of defocus values and the use of high frequency pass filter are evaluated. The obtained phase of wave function was converted to the geometric phase of the corresponding lattice planes, which was then used for the two-dimensional mapping of lattice strain following the dark-field inline holography (DIH) routine. The strain map obtained by DIH after optimized image processing is compared with that obtained by the geometric phase analysis of high resolution TEM (HRTEM) image, manifesting that DIH yields more accurate and reliable strain information than HRTEM-based GPA.