• Current Research on Agriculture and Life Sciences
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• 제10권
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• pp.137-145
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• 1992

본 연구에 사용한 시료는 형성층 유래의 캘러스를 0.01mg/${\ell}$ 2, 4-D, 0.5mg/${\ell}$ BAP, 3% sucrose, 0.75% 한천을 첨가한 MS배지에서 재분화 시킨 개체를 이용하였다. 시료의 대량증식은 1.0mg/${\ell}$ BAP를 첨가한 MS 배지에서 실시하였으며 엽전개는 식물생장조절제가 첨가되지 않은 MS배지로 옮겨서 실행하였다. Agrobacteria를 이용한 형질 전환은 엽절편, 절간조직등을 박테리아를 묻힌 침으로 자극하여 식물체 분화를 유도하였다. 그 결과 엽절편 조직에서는 분화된 식물체를 얻지 못했으나, 절간조직의 측아에서는 49%에 달하는 24개체가 분화되었다. 이들 분화된 줄기는 kanamycin이 100mg/${\ell}$이 함유된 선발 배지에서 일차적인 선발을 하여 최종적으로 GUS 유전자 검정을 한 결과 처음에 접종한 50개체중 형질전환 된 것으로 추정되는 5개체를 얻어서 형질 전환 추정 비율은 10%에 달한 것으로 나타났다.

• Journal of Applied Biological Chemistry
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• 제56권4호
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• pp.213-217
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• 2013

Phytochemical studies on the leaves of Ailanthus altissima (Simaroubaceae) have not been reported previously. Thus, the authors isolated and identified secondary metabolites from A. altissima. Dried and powdered leaves were extracted with 80% aqueous methanol, and the concentrated extract was successively partitioned with ethyl acetate, n-butanol, and water. Four flavonoids were isolated from the ethyl acetate fraction through repeated silica gel and octadecyl silica gel column chromatography. Spectroscopic data including NMR, MS, and IR allowed for identification of the chemical structures as quercetin (1), afzelin (2), quercitrin (3), and isoquercitrin (4). This is the first report of the isolation of these compounds from A. altissima. The four isolated flavonoids 1-4 as well as solvent fractions (ethyl acetate, n-butanol, and water), were evaluated for DPPH radical scavenging activity.

• 한국가구학회지
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• 제26권2호
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• pp.179-185
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• 2015

A study was carried out to observe the 1% aqueous safranine solution flow speed in longitudinal and radial directions of softwood G. biloba, ring-porous wood A. altissima, and diffuse- porouswood D. kaki. In radial direction, ray cells and in longitudinal direction tracheids, vessel and wood fiber were considered for the measurement of liquid penetration speed at less than 12% moisture contents (MC). The length, lumen diameter, pit diameter, end wall pit diameter and the numbers of end wall pits determined for the flow rate. The liquid flow in the those cells was captured via video and the capillary flow rate in the ones were measured. Vessel in hardwood species and tracheids in softwood was found to facilitate prime role in longitudinal penetration. Radial flow speed was found highest in ray parenchyma of G. biloba. Anatomical features like the length and diameter, end-wall pit numbers of ray parenchyma were found also responsible fluid flow differences. On the other hand, vessel and fiber structure affected the longitudinal flow of liquids. Therefore, the average liquid penetration depth in longitudinal tracheids of G. biloba was found the highest among all cells considered in D. kaki and A. altissima.

• 한국산림과학회지
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• 제78권4호
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• pp.412-418
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• 1989

가중나무(A. altissima, heaven tree)의 형성층(形成層) 조직(組織)에서 callus을 유도(誘導)하여 식물체(植物體)를 재분화(再分化)시키는데 필요(必要)한 제요인(諸要因)을 조사(調査)하였다. callus 유기(誘起)가 가장 좋은 배지(培地) 조합(組合)은 MS 기본배지(基本培地)에 2, 4-D 1.0 mg/l와 BAP 0.1 mg/l을 첨가(添加)한 조합(組合) 이였으며, 유기(誘起)된 callus의 증식(增殖)도 같은 조합(組合)에서 좋은 결과(結果)를 얻었다. callus에서의 재분화는 MS 기본(基本) 배지(培地)에 2, 4-D 0.01mg/l와 BAP 0.5 mg/l 첨가(添加)한 조합(組合) 배지(培地)에서 평균(平均) 5.0개의 줄기를 얻어서 가장 높은 분화율을 관찰할 수 있었다. 재분화된 줄기를 1/2 MS 기본배지(基本培地)에 이식(移植)하였을때 전부 발근(發根)되었으며, 발근(發根)된 식물체(植物體)는 pot로 이식(移植)하여 활착(活着)시켰다.

• 한국식품저장유통학회지
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• 제14권2호
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• pp.170-176
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• 2007

한방생약자원으로 사용되는 가죽나무(A. altissima)의 뿌리와 줄기, 잎의 열수 추출물을 농도에 따라 전자공여능, SOD유사활성, 아질산염 소거능을 측정하여 항산화 활성에 대하여 분석하였다. 그리고 통풍을 일으키는 원인으로 알려진 xanthine oxidase와 melanin 색소 합성에 작용하는 tyrosinase저해 활성을 측정하여 가죽나무의 생리활성을 분석하였다. 전자공여능은 1.0 mg/mL의 농도에서 뿌리(91.25%) > 줄기(67.03%) > 잎(47.94%)의 순 이었으며, SOD 유사활성능은 잎(49.07%) > 뿌리(14.31%) > 줄기(10.78%)의 순으로 나타났다. 아질산염 소거능은 pH 1.2의 조건하에서 잎에서는 93.33%, 줄기와 뿌리는 54.07%, 45.97%로 나타났으며, pH 3.0에서도 잎 추출물은 85.40%로 pH 1.2의 줄기와 뿌리 보다 높은 소거효과를 나타내었다. Xanthine oxidase 저해는 2.0mg/mL 농도에서 뿌리와 줄기가 97.44%와 97.41%, 잎에서도 92.09%였으며 1.0 mg/mL의 농도에서도 87% 이상의 높은 저해효과가 나타났다. Tyrosinase저해는 0.1mg/mL의 저농도에서 뿌리 추출물이 63.97%로 줄기(4.27%)와 잎(9.09%)보다 7배 이상 높았으며 2.0 mg/mL의 고농도에서도 뿌리는 67.38%로 줄기와 잎보다 높았으며, 농도의 증가에 따른 저해율은 큰 차이가 없었다. 이상의 결과 가죽나무는 한방생약자원으로 이용되는 뿌리와 줄기뿐만 아니라 잎도 우수한 생리활성을 나타내는 것으로 생각되며 뿌리 추출물은 소량으로도 높은 tyrosinase 저해효과를 나타내는 것으로 분석되었다.

• 동의생리병리학회지
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• 제18권6호
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• pp.1728-1732
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• 2004

• The Korean Journal of Ecology
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• 제20권3호
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• pp.181-189
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• 1997

Phenolic compounds from 7 species of naturalized invader species and Korean wild plants were analyzed by high performance liquid chromatography. Eleven phenolic compounds including benzoic acid were identified. The extract of naturalized plants was significantly more inhibitory to seed germination and seedling growth of the both naturalized and Korean wild plants. The content of total phenolic compounds in each extract were 43.5 mg/l in Ailanthus altissima as the maximum amount and 25.5 mg/l in Phytolacca americana as the minimum. Phytotoxic substances of ethanol extracts was investigated for antifungal activity against 23 selected fungus species. The antifungal activity of Phytolacca americana showed the greatest clear zone of 23 mm in Aspergillus awamori and its activity had an effect against 6 fungus species. Ailanthus altissima formed the greatest clear zone of 26 mm in Erwinia carotovora sub. sp. carotovora and had an effect agsinst 2 fungus species.

• 생약학회지
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• 제25권2호
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• pp.140-143
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• 1994

During the serial attempts to identify the chemical and biological characteristics of Ailanthi Cortex Radicis, the root bark of Ailanthus altissima (Simaroubaceae), we find out the serious toxic effect on kidney by chloroform fraction of the methanolic extract of the herb drug. The toxicities were revealed as the increase of urea nitrogen amount in blood and lactate dehydrogenase and ${\gamma}-glutamyltransferase$ activities in urine and the decrease of the concentration of glutathione and both of protein bound and non-protein bound -SH in kidney tissue.

• 생약학회지
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• 제25권1호
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• pp.47-50
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• 1994

For the biological survey, effects of Ailanthi Cortex Radicis, the root bark of Ailanthus altissima(Simaroubaceae) on epoxide hydrolyzing enzymes were checked. The methanolic extract and its chloroform fraction were shown to activate the liver metabolizing enzyme system including epoxide hydrolase system which was monitored by activities of transaminase, lactate dehydrogenase, alkaline phosphatase and epoxide hydrolase system in bromobenzene treated rats. But they showed no effect on glutathione S-transferase activity.

• Journal of Ecology and Environment
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• 제29권1호
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• pp.41-47
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• 2006

We investigated the enect of $O_3$ on the photosynthetic characteristics of tree of heaven (Ailanthus altissima) that is naturalized plant and used as restoration plant for contaminated area. Two-year-old seedlings were planted to pots and transferred into closed $O_3$ chamber. Photosynthetic pigments contents and photosynthetic characteristics were measured every three weeks under 100 pub $O_3$ fumigation. There was no visible foliar injury by $O_3$ exposure and contents of photosynthetic pigments did not show significant differences between control and $O_3$-treated seedlings. Also there were no significant differences in stomatal conductance, and water use efficiency. But photosynthetic rate and apparent quantum yield (AQY) of $O_3$ treated seedlings were reduced after nine weeks of ozone fumigation. In addition, the reduction of carboxylation efficiency and photorespiration were observed in the leave of $O_3$ treated seedlings after six weeks. In accordance with our result, carbon fixation system of A. altissima was most sensitive to $O_3$ stress to evaluate physiological damage induced by $O_3$.