• Asian-Australasian Journal of Animal Sciences
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• v.17 no.9
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• pp.1192-1196
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• 2004

In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences in gene expression of pig's Longissimus dorsi between the high-parent heterosis cross combination Landrace${\times}$Large White and the mid-parent heterosis cross combination Large White${\times}$Meishan. Three pig purebreds, Large White, Meishan, and Landrace and four types of reciprocal $F_1$ hybrids were analyzed using nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 7,000 reproducible bands were examined. The patterns of gene expression of each cross combination were analyzed and eight common patterns (fifteen kinds) were found. When the results from the two cross combinations were put together and compared, eight different typical expression patterns were observed, these indicated that the patterns of gene expression of these two cross combinations had obvious differences. Gene expression correlation and cluster analyses of the two cross combinations indicated that the gene expression of the mid-parent heterosis cross combination was correlated with maternal effect, but in the high-parent heterosis cross combination, paternal effect acted in the gene expression of the hybrids or the gene expression of the hybrids was biased towards one parent.

• Magazine of the Korean Society of Agricultural Engineers
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• v.25 no.4
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• pp.38-49
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• 1983

This study was intended to develop a curriculum for the agricultural engineering technology, and that on confidence with junior college characteristics. In order to per- formance with this study, job cluster was analyzed on 28 parts in respect to the agricultural engineering field and questionnaire was investigated to the field including in alumni 181, students 537, professional workers 174 and professors 54. The results from the above analysis and investigation can be summarized as follows. 1. According to the investigation on degree of important subjects from the questionnaire, it is presented at mathematics 43%, physics 36% in students and 32%, 12% in professional workers. Considering from this results, it is desirable that primary subjects should be forced. 2. In comparing with fundamental major subjects and applied it, it is presented at that 41%, this 34%, in the total investigations. It can be found that in the degree of requirement, this is lower than that among the major subjects. 3. In investigation to the degree of requirement in the experiment and practice, it is presented at surveying 63%. Soil mechanics 14%, materials 13%, hydraulics 9% in order, in total inspection. 4. From investigation to the degree of requirement on the subjects of established newly, it is presented at majoring and living English 64%, E.D.P.S 30%, in the total questionnaire. Judging from the results, it seems that the foreign language is necessary to technician more and more, for advance to abroad. Also, it is no wonder that make good use of the E.D.P.S. 5 The table 5 is represented the model curriculum from the above results in anaized data on the questionnaire and the job cluster of table 2.

• Journal of Agricultural Extension & Community Development
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• v.19 no.2
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• pp.211-240
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• 2012

The purpose of this study was to identify korean agricultural extension workers' Perception of role ambiguity according to restructuring of Agricultural Administrative Systems. The target population of this study was selected from 21 centers out of 156 centers. The subjects for study were 728 agricultural extension officers by the cluster sampling drawn from the sampled 21 agricultural extension centers in four clusters. A survey questionnaire was utilized to measure variables of this study. It consisted of role ambiguity scale and demographic items. In this study, role ambiguity scale was translated from the existing scale. Through a pilot test and final survey, reliability and validity of these scales were examined. The data were collected by mail. A total 420 questionnaires out of 728 from 21 agricultural extension centers were returned. After data cleaning, 410 responses were used for analysis. Both descriptive and inferential statistics were applied for data analysis. Major findings of the study were as follows. There were significant differences of the degree of role ambiguity among the local extension workers divided by four clusters. Especially, the degree of role ambiguity of restructured local administrative system were more higher than other clusters, comparatively. Thus, the factors causing the role ambiguity to agricultural extension officers need to be measured it in-depth.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.138.1-138
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• 2003

Potato mop-top virus(PMTV) was identified from Solanum tuberosum cv. Gawon showing bright chlorotic mottle symptom in Namwon, Korea. Samples were collected green-house in February, 2003. Electron microscopic examination of negatively stained preparation revealed that PMTV were rigid-rod shaped particles about 100-150, 250-300 nm x 18-20 nm in length. In ultrathin sections of leaf tissue from diseased potato plants, cluster of viruses particles were observed in the cytoplasm. TAS-ELISA determined that the virus was serologically related to PMTV. PMTV produced double ring necrotic local lesion in inoculated leaf of Chenopodium amaranticolor in incubated at 15$^{\circ}C$. The PMTV could be detected with RT-PCR using PMTV detectable primer set designed to amplify about 540 bp of the partial CP gene of PMTV

• Korean Journal of Medicinal Crop Science
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• v.10 no.5
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• pp.333-339
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• 2002

This study was conducted to obtain the basic data for practical use of the Polygonatum genetic resources. The 20 collections were analyzed by principal component analysis of 8 characters and cluster analysis. In the principal analysis, the first, the second and the third components contributed 54.10%, 18.95% and 11.62% of the variations, respectively. The cumulative contribution from the first to the third principal components was 84.68%. The first principal component was related to shape and size of plant, and assimilatory, reserve and reproductive organs. The second principal component was related to growth and development of plant, and reserve organ. And the third principal component was related to growth and development of plant. Based on cluster analysis, the 20 collections were classified into 4 distinct groups with the average distance greater than 0.7 between groups. Group I was Polygonatum sibiricum $D_{ELAR}$ and Group II included P. odoratum var. pluriflorum $O_{HWI}$, P. odoratum var. pluriflorum $O_{HWI}$ for 'Variegatum' Y. Lee, for. nov., P. odoratum var. thunbergii $H_{ARA}$ and P. odoratum var. maximowiczii $K_{OIDZ}$. GroupIII was P. involucratum $M_{AXIM}$, P. desoulavyi $K_{OMAROV}$ and P. humile $F_{ISHER}$ ex. $M_{AXIM}$. And GroupIV included P. lasianthum var. coreanum $N_{AKAI}$ and P. inflatum $K_{OMAROV}$.

• Korean Journal of Plant Resources
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• v.31 no.1
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• pp.71-76
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• 2018

This study was conducted to investigate the effect of shoot twist on fruitfulness and fruit quality of 'Campbell Early' grapevine. Proper pruning and training are essential to produce a good yield of high-quality fruit and to maintain the balance between vegetative growth and fruiting. The most common problem in spur-pruned 'Campbell Early' cultivar is that vigorous buds has low fruitfulness and thereby the shoot become more vigorous the following spring because of lower crop load. Therefore, shoot twists in very vigorous 'Campbell Early' canes (above 10.0 mm) were performed on the third nodes and the $7^{th}$ nodes of each shoot at 7 days before bloom and full bloom, respectively. Sprouting date, blooming date were not significantly different among the treatments while, harvesting date was delayed approximately 3 days. However, number of berries per cluster, cluster weight and fruitfulness were significantly higher in the shoot twist treatment on the third nodes than the control that was topping alone. Combination treatments of shoot twist and topping had an additive effect on increasing cluster weight resulting in higher increase of yield by 12.1 ㎏ per vine. These results indicated that the shoot twist on very vigorous canes of 'Campbell Early' grapevine for well fruitfulness seemed to be very effective.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.4
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• pp.235-244
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• 2004

In this study, we compared the levels of methylotrophic bacterial community diversity in the leaf samples of 19 rice cultivars collected from three regions of Korea. Nineteen pink pigmented isolates showing characteristic growth on methanol were obtained. Physiological and biochemical characters of each isolate were examined according to methods described in Bergey's Manual of Systematic Bacteriology. When phylotypes were defined by performing numerical analysis of 37 characteristics, four distinct clusters were formed. The two reference strains, Methylobacterium extorquens AM1 and Methylobacterium fujisawaense KACC10744 were found to group under cluster IV and cluster III respectively. Cluster I diverged on the basis of nitrate reduction and four isolates showed tolerance upto 0.5 M NaCl concentrations. Two strains in cluster I and III were found to possess methane utilizing properties. Most of the isolates in all the four clusters utilized monosaccharides, disaccharide and polyols as carbon source. When the isolates were subjected for indole-3-acetic acid (IAA) analysis in the presence of L-tryptophan, only 8 isolates exhibited IAA production. In addition, the nitrogen source in the medium was found to influence the IAA production. Addition of $(NH_4)_2SO_4$ in the medium led to a 2 to 30 fold increase in the indole synthesis. However, $KNO_3$, $NH_4NO_3$ and $NH_4Cl$ substitution did not significantly stimulate the synthesis of IAA in the growth medium. Result of gnotobiotic root elongation assay significantly increased roots and shoots lengths, and number of lateral roots, which is mediated by IAA production in the culture medium. The rice seedlings primary roots from seeds treated with methylotrophic isolates were on average 27 to 56% longer than the roots from seeds treated with the uninoculated seeds. In addition, application of different high concentrations of authentic IAA ($400g\;mL^{-1}$) to roots of rice seedlings inhibited root growth. However, the IAA concentration from 10 to $200g\;mL^{-1}$, IAA promoted root growth of rice seedlings. These results suggest that bacterial IAA plays a major role in the development of the host plant root system.

• Microbiology and Biotechnology Letters
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• v.45 no.1
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• pp.71-80
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• 2017

Tea is the most popular beverage in the world. The three main types are green, black, and post-fermented. Post-fermented teas are produced by the microbial fermentation of sun-dried green tea leaves (Camellia sinensis). In this study, the composition of the bacterial communities involved in the production of traditional oriental post-fermented teas (Korean algacha, dancha, and Chinese pu-erh) were investigated using 16S rRNA gene analysis. The dominant microorganisms present in the post-fermented teas included the ${\alpha}$-proteobacteria Rhodobacteraceae and Sphingomonas, and the ${\gamma}$-proteobacteria Pantoea. Cluster analysis confirmed that the microbial populations present in both Korean and Chinese post-fermented teas grouped into the same class. Interestingly, the dominant microorganism present in the Korean post-fermented teas was a bacterium, while for the Chinese post-fermented tea, it was a fungus.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.66.1-66
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• 2003

The aim of this study was to characterize the interactions of rice and Burkholderia glumae, a causal agent of bacterial grain rot of rice, at molecular levels using whole genomic sequences and to identify genes important for pathogenicity and symptom development. To do these, we sequenced whole genome of the bacterium and constructed cosmid clone profiles. We generated pools of mutants using various transposons and determined mutation sites by sequencing rescued plasmids. We focused on studying toxoflavin biosynthetic genes, quorum sensing regulation, and Hrp type III protein secretion systems. We found that two possible operons consisting of five genes are involved in toxoflavin biosynthesis and their expression is regulated by quorum sensing and LysR-type regulator, ToxR. We have isolated the nn PAI of B. glumae and characterized by mutational analyses. The hrp cluster resembled most the putative Type III secretion systems of B. pseudomallei, which is the causative agent of melioidosis, a serious disease of man and animals. The Hrp PAI core region showed high similarity to that of Ralstonia solanacearum and Xanthomonas campestris, however some aspects were dissimilar.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.1
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• pp.7-12
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• 2022

Farming practices that balance environmental friendliness with biodiversity are increasingly valuable. Wild plants on farmlands compete for nutrients with crops and create a crucial microhabitat and resources for animals such as natural enemies. Investigating farmlands and their surrounding plants with limited human and material resources has become an essential aspect of evaluating the agricultural ecosystem services. This study investigated plants in six agricultural long-term ecological research sites in Taiwan from 2017 to 2020 to determine the ideal season for investigation. Cluster analysis was performed to group habitats with similar plant composition, and the species-area curves of the clusters in each season were created. The results indicated that the agricultural ecosystem could be divided into farmlands, banks, orchards, and tea gardens. The habitats were divided into farmland, bank, Chia-Yi orchard, Gu-Keng orchard, and tea garden clusters. Ground plant cover can be investigated all year with at least 18 quadrats. However, if human and material resources are limited, 10 quadrats should be the minimum for farmlands in autumn and for the other microhabitats in spring. The minimum number of quadrats is 10 for banks, 17 for orchards, and 9 for tea gardens.