• Title/Summary/Keyword: Agri-food

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Growth Potential of Escherichia coli O157:H7 in Lettuce Leaf Extract and on Lettuce Leaf Surface (상추잎 즙액과 표면에서 Escherichia coli O157:H7의 생장 가능성)

  • Kim, Won-Il;Jung, Hyang-Mi;Kim, Se-Ri;Park, Kyeong-Hun;Kim, Byung-Seok;Yun, Hye-Jung;Yun, Jong-Chul;Ryu, Kyoung-Yul
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.296-301
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    • 2011
  • This study was to evaluate the growth potential of E. coli O157:H7 in lettuce leaf extracts and on lettuce leaf surface at various temperatures. The pathogen can survive and multiply in the extracts and leaf surface of lettuce. The population of E. coli O157:H7 in the lettuce extracts reached to 4.79 log CFU/mL at $37^{\circ}C$. The multiplication of pathogen in lettuce extracts initiated within 10 hours of inoculation over $15^{\circ}C$ conditions. And it can survive in the lettuce leaf extracts at $4^{\circ}C$ for 100 hours at least. And this pathogen can multiply on lettuce leaf surface and the population of pathogen on the lettuce leaf surface increased to 1.82 log CFU/g at $25^{\circ}C$. At $37^{\circ}C$, the pathogen density increased to 1.53 CFU/g within 3 days after inoculation. At all temperature, irrespective of the inoculation level, similar trends in growth of E. coli O157:H7 were observed. These results emphasize the growth potential of E. coli O157:H7 in lettuce leaf extract and on lettuce leaf surface. To reduce the risk of outbreak, it is important to maintain the cold chain system during storage before the consumption.

Changes of nutritional constituents and antioxidant activities by the growth periods of produced ginseng sprouts in plant factory (식물공장에서 생산된 새싹인삼의 생육 시기에 따른 영양성분 및 항산화 활성 변화)

  • Seong, Jin A;Lee, Hee Yul;Kim, Su Cheol;Cho, Du Yong;Jung, Jea Gack;Kim, Min Ju;Lee, Ae Ryeon;Jeong, Jong Bin;Son, Ki-Ho;Cho, Kye Man
    • Journal of Applied Biological Chemistry
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    • v.65 no.3
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    • pp.129-142
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    • 2022
  • Ginseng sprouts, which can be eaten from leaves to roots, has the advantage of not having to use pesticides without being affected by the season by using smart farms. The optimal cultivation timing of sprout ginseng was checked and the nutritional content and antioxidant activity were compared and analyzed. The values of total fatty acids and total minerals were no significant changes during the growth periods. The contents of total amino acids were slightly decreased to 45 days and after increased to 65 days. When the growth period was 65 days, arginine had the highest content of 3309.11 mg/100 g. The total phenolic contents were high at 3.73 GAE mg/g on the 45 days, and the total flavonoid contents were also the highest at 9.04 RE mg/g on the 45 days. The contents of total ginsenoside was not noticeable for the growth periods (29.83 on 25 days→32.77 on 45 days→26.02 mg/g on 65 days). The ginsenoside Rg2 (0.62 mg/g), Re (8.69 mg/g), Rb1 (4.75 mg/g) and Rd (3.47 mg/g) had highest contents on 45 days during growth. The values of phenolic acids and flavonols were gradually increased to 45 days (338.6 and 1277.14 ㎍/g) and then decreased to 65 days. The major compounds of phenolic acids and flavonols were confirmed to benzoic acid (99.03-142.33 ㎍/g) and epigallocatechin (416.03-554.64 ㎍/g), respectively. The values of 2,2-diphenyl-1-picrylhydrazyl (44.27%), 2,4,6-azino-bis (3-ethylbenzothiazoline-6-sulphnoic acid) diammonium salt (75.16%), and hydroxyl (63.29%) radical scavenging activities and ferric reducing/antioxidant power (1.573) showed the highest activity on the 45 days as well as results of total phenolic and total flavonoid contents.

Impacts of Different Pentachloronitrobenzene (quintozene) Use Patterns on Severity of Damping-off of Ginseng (Panu quinquefolitrs)

  • Reeleder, R.D;Capell, B
    • Journal of Ginseng Research
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    • v.26 no.1
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    • pp.10-16
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    • 2002
  • In replicated field trials, the efficacy of pentachloronitrobenzene (PCNB; quintozene) for control of damping-off of ginseng seedlings was found to be affected by timing of application and formulation. Application at the time of seeding and prior to placement of straw mulch was found to provide the moat consistent level of disease control. However, decline in plant stand during the four-year production cycle resulted in most treatments providing similar levels of plant populations at harvest. Soil residues of pentachloronitrobenzene were generally highest (1 $\mu\textrm{g}$ PCNB/g soil) in those treatments that exhibited the highest levels of disease control in the seedling year. Straw contained high levels of quintozene after application. Beet seed assays with artificially-infested soils indicated that current use rates provide an amount of product suitable for high levels of disease control.

Microbial Hazard Analysis of Astragalus membranaceus Bunge for the Good Agricultural Practices (농산물우수관리를 위한 황기(Astragalus membranaceus Bunge)의 미생물학적 위해요소 분석)

  • Kim, Yeon Rok;Lee, Kyoung Ah;Kim, Se-Ri;Kim, Won-Il;Ryu, Song Hee;Ryu, Jae-gee;Kim, Hwang-Yong
    • Journal of Food Hygiene and Safety
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    • v.29 no.3
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    • pp.181-188
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    • 2014
  • The objective of this study was to analyze the microbiological hazards of Astragalus membranaceus Bunge on the post-harvest processing. Samples from processing equipments (cleaner, water, cart, table, tray and packaging machine), personal hygiene (hand) and harvested crops (before washing, after washing, after sorting, and after drying) were collected from four farms (A, B, C, and D) located in Chungchengbuk-do, Korea. The samples were analyzed for sanitary indication bacteria and pathogenic bacteria. First, total aerobic bacteria and coliform in processing facilities were detected at the levels of 0.93~4.86 and 0.33~2.28 log CFU/$100cm^2$ and/mL respectively. In particular, microbial contamination in hand (5.43~6.11 and 2.52~4.12 log CFU/Hand) showed higher than processing equipments. Among the pathogenic bacteria, Bacillus cereus was detected at the levels of 0.33~2.41 log CFU/$100cm^2$, 1.48~3.27 log CFU/Hand and 0.67~3.65 log CFU/g in equipments, hands, and plants and Staphylococcus aureus were detected in cleaner, table, hand and harvested crops (before washing and after sorting) by qualitative test. Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella spp. were not detected. These results indicated that personal hygiene and processing equipments should be managed to reduce the microbial contamination of A. membranaceus Bunge. Therefore, management system such as good agricultural practices (GAP) criteria is needed for hygienic agricultural products.

Comprehensive comparison of the primary and secondary metabolites and antioxidant activity of Polygoni multiflori Radix by processing methods (가공 방법에 따른 하수오의 영양성분 및 항산화 활성의 종합적인 비교)

  • Hee Yul Lee;Chung Eun Hwang;Kyung Pan Hwa;Du Yong Cho;Jea Gack Jung;Min Ju Kim;Jong Bin Jeong;Mu Yeun Jang;Kye Man Cho
    • Journal of Applied Biological Chemistry
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    • v.65 no.4
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    • pp.287-298
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    • 2022
  • This study investigated the changes in the physiochemical property, phytochemical content, nutritional content and antioxidant activity of Polygoni multiflori Radix by steam, aging, and fermentation. After processing Polygoni multiflori Radix (PMR), pH slightly decreased, while acidity increased (pH 5.70→4.78, acidity 0.23→0.29%). The reducing sugar content increased after aging and fermentation from 1.19 mg/g (PMR) to 1.40 (fermented PMR, FPMR), 1.30 (red PMR, RPMR), 1.53 (fermented red PMR, FRPMR), 1.99 (black PMR, BPMR), and 2.33 mg/g (fermented black PMR, FBPMR). Total phenolic content was highest in PMR (6.05 mg/g) and total flavonoids and maillard product were increased after aging and fermentation of PMR, and were the highest in BPMR (1.60 mg/g) and FBPMR (2.76 O.D.), respectively. The major phytochemical was 2,3,5,4'-tetrahydroxystilbene-2-0-α-glucoside, which were highest in PMR (64.9 mg/g) with 46.47 mg/g at FPMR, 33.94 mg/g at RPMR, 48.76 mg/g at FRPMR, 36.68 mg/g at BPMR and 34.35 mg/g at FBPMR. The main fatty acids and free amino acids were detected as palmitic acid (C16:0) and proline, respectively. Generally, 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical scavenging activities and FRAP reducing powers were shown high in PMR (39.06%, 98.32%, and 2.61 O.D. in extracts concentration 1.0 mg/mL), then were decreased after aging and fermentation.

Trends of Quality Management Technologies and Applications in Agri-Food Industry in EU (EU의 농식품 품질관리 기술 활용 현황)

  • Kim, Jong-Hoon
    • Bulletin of Food Technology
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    • v.23 no.4
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    • pp.592-597
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    • 2010
  • 본 내용은 2010년 4월 25일부터 2010년 5월 1일까지 이탈리아의 UNITEC, SALVI, SORMA, APOFRUIT, 농식품유통매장을 방문하여, EU의 농식품 선별 및 품질관리 센싱기술의 현장 활용실태와 농식품 이력추적관리 및 농산물산지유통센터의 품질센싱 기술을 조사한 결과이다.

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Susceptibility of Spodoptera exigua to UVA Insecticides Using Agricultural Multi-copter on Cabbage Field (농업용 멀티콥터를 활용한 무인항공기용 작물보호제에 대한 배추 파밤나방의 약제감수성)

  • Park, Bueyong;Lee, Sang-Ku;Jeong, In-Hong;Park, Se-Keun;Lee, Sang-Bum;Kim, Gil-Hah
    • Korean journal of applied entomology
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    • v.58 no.4
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    • pp.271-280
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    • 2019
  • We investigated the control efficacy and phytotoxicity of unmanned aerial vehicle-applied pesticides against the insect Spodoptera exigua, a major emerging pest in Chinese cabbage. Phytotoxicity was investigated in cabbage and 7 crops cultivated in the perconducted on 8 surrounding crops including Chinese cabbage at 1 to 2 times the recommended pesticide dosage. We treated cabbage fields with spinetoram suspension concentrate (16×), methoxyfenozide, sulfoxaflor suspension concentrate (16×). Then, we used water-sensitive paper to measure the distribution pattern of falling pesticide particles and the degree of coverage. Two of the pesticides showed 97% control efficacy, however, control efficacy might differ in resistant populations. Phytotoxicity was not observed in Chinese cabbage and the 7 surrounding crops treated with 1 to 2 times the recommended pesticide dosage. Analysis of the distribution pattern of falling pesticide particles revealed that breeze caused particle diffusion. Thus, wind is an important factor affecting the uniform treatment and diffusion of multicopter-applied pesticides. It follows that setting optimal conditions is necessary for effective control and treatment.

Quality characteristics of Tteokbokki (Rice Cake) depending on cultivars and particle sizes of dry-milling rice flour

  • Song, Eun-Ju;Choi, So-Ra;Song, Young-Eun;Lee, In-Sok;Lee, Ki-Kwon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.276-276
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    • 2017
  • This study was examined to establish a conditions for producing Tteokbokki using dry-milling rice flour which can save manpower and labor time. Since the cost of producing rice flour milled in a wet condition is 500 to 700 won/kg, which is more than twice as much as that of 300 won wheat flour, it is necessary to directly make rice flour from raw rice. The dry-milling rice flour used in the experiment was produced by Air mill (Nara machinary co. ltd., Tokyo, Japan) from 5 rice cultivars (Samkwang, Dasan-1, Boramchan, Seolgaeng, Hanareum-2), which were cultivated in A-san in 2015 year. Their particle sizes were 50, 100 and $150{\mu}m$ for each cultivar, respectively. A control was a wet-milled rice flour milled with a roll mill after the rice was soaked in water for 4 hours. The moisture content of dry-milling rice flour based on cultivars was 11 ~ 12%, and added water up to 50~55% of dry-milling rice flour weight. The RVA characteristic of peak viscosity was the highest in Dasan-1 and Hanareum-2, the lowest in Seolgaeng. The setback value used as an indicator of aging was the highest in Dasan-1, therefore Dasan-1 was expected to be quick solidification, resulting in the low tendency of sensory evaluation. The damaged starch was high in Dasan-1 and Boramchan (p<0.05) compared to others. The Hunter color L were no significant among cultivars and b value increased in all cultivars of dry-milling rice flour compared with control. The hardness of dry-milling rice flour was higher than that of the control, especially Dasan-1 and Hanareum-2 were the highest. Based on the sensory evaluation, the best cultivars were Boramchan, Hanareum-2 and Samkwang. The overall preference of dry-milling rice flour was good in particle size of 50~100um.

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Insecticidal activities and repellent effects of methylcinnamate and essential oils from Alpinia galangal against nymphs and adults of Metcalfa pruinosa (양강근(Alpinia galangal Swartz) 정유와 양강근 유래 주요물질인 Methylcinnamate의 미국선녀벌레(Metcalfa pruinosa Say)에 대한 살충 및 기피 효과)

  • Park, Bueyong;Lee, Sang-Ku;Jeong, In-Hong;Park, Se-Keun;Lee, Sang-Bum
    • Journal of Applied Biological Chemistry
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    • v.61 no.3
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    • pp.291-295
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    • 2018
  • Metcalfa pruinosa is a pest causing widespread problems to many crops in Korea. This pest infects fruit crops especially, persimmon and grapes. We tested the possibility of M. pruinosa management using essential oils of Alpina galangal and methylcinnamate which were extracted from A. galangal by steam distillation method. The use of essential oil showed a mortality rate of 10.0 and 23.3% for adults and nymphs, respectively. While the use of methylcinnamate resulted to a mortality rate of 40.0% in adults and 36.6% in nymphs. For its repellent effect, it showed an avoidance rate of 50.0 and 63.3% for adult and nymph respectively. Considering these two results, the extract of A. galangal are shown to have some synergic effect for pest control. The result of this study showed a possibility of M. pruinosa control using essential oil and methylcinnamate from A. galangal.