• 한국항해항만학회지
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• 제38권4호
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• pp.421-427
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• 2014

In order to protect coastal facilities mainly from wave and current actions, the self-locking eco blocks constituting elements of protecting shore structures against scouring were designed. These blocks are adapted to the sloping bottom, coastal dunes, and submerged coastal pipelines, counteracting the destructive and erosive impulse action. A series of laboratory experiments has been conducted to investigate the reflection of water waves over and against a train of protruded or submerged shore structures and compare the reflecting capabilities of incident waves including wave forces. In this study the hydraulic model experiment was conducted to identify the performance of newly designed water affinity eco blocks to keep the coast slope and bottom mound from scouring by reduction of the wave reflection and to convince stability of the block placement. Revised design of each block element was also tested for field conditions. From the result of experiments, the field applicability of the developed blocks and placement was discussed afterward.

• BMB Reports
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• 제31권4호
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• pp.307-327
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• 1998

Cytochrome c peroxidase (CcP) is a yeast mitochondrial enzyme which catalyzes the reduction of hydrogen peroxide to water using two equivalents of ferrocytochrome c. The CcP/cytochrome c system has many features which make it a very useful model for detailed investigation of heme protein structure/function relationships including activation of hydrogen peroxide, protein-protein interactions, and long-range electron transfer. Both CcP and cytochrome c are single heme, single subunit proteins of modest size. High-resolution crystallographic structures of both proteins, of one-to-one complexes of the two proteins, and a number of active-site mutants are available. Site-directed mutagenesis studies indicate that the distal histidine in CcP is primarily responsible for rapid utilization of hydrogen peroxide implying significantly different properties of the distal histidine in the peroxidases compared to the globins. CcP and cytochrome c bind to form a dynamic one-to-one complex. The binding is largely electrostatic in nature with a small, unfavorable enthalpy of binding and a large positive entropy change upon complex formation. The cytochrome c-binding site on CcP has been mapped in solution by measuring the binding affinities between cytochrome c and a number of CcP surface mutations. The binding site for cytochrome c in solution is consistent with the crystallographic structure of the one-to-one complex. Evidence for the involvement of a second, low-affinity cytochrome c-binding site on CcP in long-range electron transfer between the two proteins is reviewed.

• 대한토목학회논문집
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• 제5권2호
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• pp.59-66
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• 1985

동적(動的) 인장하중(引張荷重) 받는 콘크리트의 거동(擧動) 모델화(化)하기 위한 비선형(非線型) 구성이론(構成理論)이 제안(提案)되었다. 콘크리트의 정적(靜的) 인장거동(引張擧動)은 징세균열면처리론(徵細龜裂面處理論)에 의(依)하여 모델하고, 동적(動的) 인장하중(引張荷重)으로 인한 변형율속도효과(變形率速度效果)를 고려하기 위하여 유사변환을 이용(利用)하였다. 본(本) 모델의 결과(結果)를 현존하는 여러 동적(動的) 인장실험자료(引張實驗資料)와 비교하였다. 또한, 동적(動的) 인장하중(引張荷重)으로 인한 인장강도의 증가현상을 예측하기 위한 공식(公式)이 제안되었다. 본(本) 모델은 콘크리트구조물(構造物)의 좀 더 실제적인 동적(動的) 유한요소해석(有限要素解析)을 가능케 한다.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1191-1197
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• 2007

The detection and kinetics of mucosal pathogenic bacteria binding on polysaccharide ligands were studied using a surface plasmon resonance biosensor. The kinetic model applied curve-fitting to the experimental surface plasmon resonance sensorgrams to evaluate the binding interactions. The kinetic parameters for the mucosal pathogenic bacteria (Pseudomonas aeruginosa, Pseudomonas fluorescens, Serratia marcescens) with the alginate ligand were determined from a kinetic model. In addition, the binding interactions of the mucosal pathogenic bacteria with polysaccharide binding pairs (Pseudomonas aeruginosa/alginate, Streptococcus pneumoniae/pneumococcal polysaccharide, Staphylococcus aureus/pectin) were also compared with their kinetic parameters. The rate constants of association for Pseudomonas aeruginosa with the alginate ligand were higher than those for Pseudomonas fluorescens. Serratia marcescens had no detectable interaction with the alginate ligand. The adhesion affinity of Pseudomonas aeruginosa with alginate was higher than that for the other binding pairs. The binding affinities of the pathogenic bacteria with their own polysaccharide were higher than that of Staphylococcus aureus with pectin. Measuring the contact angle was found to be a feasible method for detecting binding interactions between analytes and ligands.

• 한국조경학회지
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• 제27권3호
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• pp.69-79
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• 1999

Recently, new concept and paradigm of 'Environmentally-Friendless' is taking a growing interest in environmental planning and design. This study is to develop the new approach of sustainable development, and to establish the indicators for environmentally-friendliness of "Tourism Farms" in rural areas. A questionnaire survey was conducted for deputy manager group and expert group. The environmentally-friendliness of tourism farms is composed of three categories, conservation of global environment(Low Impact), friendliness to surrounding nature(High Contact), and environmental health and amenities (Health '||'&'||' Amenity). Four indicators, such as saving of energy and water resource, reduction and reuse of garbage, natural purification of sewage disposal, and utilization of natural energy, were affecting the first category, i.e., conservation of global environment(Low Impact). And, friendliness to surrounding nature (High Contact) is affected by 3 indicators, such as contact to nature and diverse green areas, water intimate '||'&'||' contact areas, and natural ecology observation by biotope. Finally, the dimension of environmental health and amenity is affected by 3 indicators, such as nature affinity by farming experience, environmental-friendliness of soil '||'&'||' crops by organic farming, campaign and education programs of environmentally-friendliness. From the result of Importance-Performance Analysis(IPA) for 10 indicators, environmentally-friendliness was recommended as 'Concentrate Here'. And, the content validity of 10 indicators for 3 categories was examined by factor analysis. The result showed as the same as hypothetical model, which proved the validity of hypothetical model.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2004년도 임시총회 및 추계학술발표회
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• pp.219-222
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• 2004

Removal characteristics of Mn and Co was studied from the contaminated solutions via surface reaction with various calcium carbonate (calcite). Synthetic calcium carbonates which has different surface morphology as well as surface areas were prepared by a spontaneous precipitation method and used. Mn and Co removal behavior by the different solid surface demonstrate characteristic sorption behaviors depend on the type of calcite used, such as surface area or surface morphology. Calcium carbonate crystals (mostly calcite) which exhibit complicated surface morphology (c-type) shows strong sorption affinity for Mn and Co removal via sorption than on the a-type or b-type calcite crystals of less complicated surfaces. The applicability of two kinetic models, the pseudo-first-order kinetic equation and the Elovich kinetic model was examined on these sorption behavior. Elovich kinetic model was found more suitable to explain the very early stage adsorption kinetics, while the pseudo-first-order kinetic equation was successfully fitted for the adsorption kinetics after 50 hours.

• Mass Spectrometry Letters
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• 제4권4호
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• pp.71-74
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• 2013

In the present study, we report that the charge-directed (assisted) peptide dissociation products, such as b- and y-type peptide backbone fragments, were the major products in MS/MS and $MS^3$ applications of some o-TEMPO-Bz-C(O)-peptide ions, while radical-driven dissociation products, such as a/x and c/z-type fragments, were previously shown to be the major products in the free radical initiated peptide sequencing mass spectrometry (FRIPS MS). Those o-TEMPO-Bz-C(O)-peptides share a common feature in their sequences, that is, the peptides do not include an arginine residue that has the highest proton affinity among free amino acids. The appearance of b- and y-type fragments as major products in FRIPS MS can be understood in terms of the so-called "mobile-proton model". When the proton is highly mobilized by the absence of arginine, the chare-directed peptide dissociation pathways appear to be more competitive than the radical-driven dissociation pathways, in our FRIPS experiments.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.458-463
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• 2018

The phosphorylation of JNK is known to induce insulin resistance in insulin target tissues. The inhibition of JNK-JIP1 interaction, which interferes JNK phosphorylation, becomes a potential target for drug development of type 2 diabetes. To discover the inhibitors of JNK-JIP1 interaction, we screened out 30 candidates from 4320 compound library with In Cell Interaction Trap method. The candidates were further confirmed and narrowed down to five compounds using the FRET method in a model cell. Among those five compounds, Acebutolol showed notable inhibition of JNK phosphorylation and elevation of glucose uptake in diabetic models of adipocyte and liver cell. Structural computation showed that the binding affinity of Acebutolol on the JNK-JIP1 interaction site was comparable to the known inhibitor, BI-78D3. Our results suggest that Acebutolol, an FDA-approved beta blocker for hypertension therapy, could have a new repurposed effect on type 2 diabetes elevating glucose uptake process by inhibiting JNK-JIP1 interaction.

• Tribology and Lubricants
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• 제30권6호
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• pp.378-383
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• 2014

This study predicts the of the hysteretic friction of a rubber block sliding on an SMA asphalt road. The friction of filled rubber on a rough surface is primarily determined by two elements:the viscoelasticity of the rubber and the multi-scale perspective asperities of the road. The surface asperities of the substrate exert osillating forces on the rubber surface leading to energy dissipation via the internal friction of the rubber when rubber slides on a hard and rough substrate. This study defines the power spectra at different length scales by using a high-resolution surface profilometer, and uses rubber and road surface samples to conduct friction tests. I consider in detail the case when the substrate surface has a self affine fractal structure. The theory developed by Persson is applied to describe these tests through comparison with the hysteretic friction coefficient relevant to the energy dissipation of the viscoelastic rubber attributable to cyclic deformation. The results showed differences in the absolute values of predicted and measured friction, but with high correlation between these values. Hence, the friction prediction model is an appropriate tool for separating the effects of each factor. Therefore, this model will contribute to clearer understanding of the fundamental principles of rubber friction.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2021년도 춘계학술대회
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• pp.597-599
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• 2021

비즈니스에 성공한 디자인 혁신 제품사례가 증가함에 따라 디자인 혁신성에 대한 관심도가 높아지고 있다. 본 연구는 블루투스 스피커 제품의 디자인 혁신성 (기능성, 인간공학, 심미성)이 구매고객의 호감도와 구매 의도에 어떠한 영향을 미치는지 살펴보고자 한다. 실증적 연구를 위한 합리적 행위이론(TRA)과 기술수용모델(TAM)을 통한 연구모형을 제언한다. 설문은 디자인 혁신성, 호감도, 구매 의도의 영향 관계를 파악하기 위한 문항으로 구성되었다. 연구결과 기능성, 인간공학, 심미성은 제품의 호감도와 구매 의도에 영향을 주었다. 블루투스 스피커 디자인 혁신 제품이 초기시장에서 수용되기 위해서는, 기능과 심미성을 중심으로 호감도에 대한 긍정적인 태도를 형성하는 것이 무엇보다 중요하다. 호감도는 디자인 혁신 제품의 구매 의도에 가장 결정적인 영향을 미치는 요인으로서 기업은 호감도에 긍정적인 영향을 미치는 다양한 요인을 발굴, 강화해야 한다.