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http://dx.doi.org/10.5478/MSL.2013.4.4.71

Charge-Directed Peptide Backbone Dissociations of o-TEMPO-Bz-C(O)-Peptides  

Jeon, Aeran (Department of Chemistry, Sogang University)
Lee, Ji Hye (Department of Chemistry, Sogang University)
Kwon, Hyuk Su (Department of Chemistry, Sogang University)
Park, Hyung Soon (Diatech Korea Co. Ltd.)
Moon, Bong Jin (Department of Chemistry, Sogang University)
Oh, Han Bin (Department of Chemistry, Sogang University)
Publication Information
Mass Spectrometry Letters / v.4, no.4, 2013 , pp. 71-74 More about this Journal
Abstract
In the present study, we report that the charge-directed (assisted) peptide dissociation products, such as b- and y-type peptide backbone fragments, were the major products in MS/MS and $MS^3$ applications of some o-TEMPO-Bz-C(O)-peptide ions, while radical-driven dissociation products, such as a/x and c/z-type fragments, were previously shown to be the major products in the free radical initiated peptide sequencing mass spectrometry (FRIPS MS). Those o-TEMPO-Bz-C(O)-peptides share a common feature in their sequences, that is, the peptides do not include an arginine residue that has the highest proton affinity among free amino acids. The appearance of b- and y-type fragments as major products in FRIPS MS can be understood in terms of the so-called "mobile-proton model". When the proton is highly mobilized by the absence of arginine, the chare-directed peptide dissociation pathways appear to be more competitive than the radical-driven dissociation pathways, in our FRIPS experiments.
Keywords
Charge-directed dissociations; Peptide; Free radical initiated peptide sequencing; Mobile proton model; Radical chemistry;
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