• Title/Summary/Keyword: Activity Support

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Activity and Stability of Immobilized Enzyme on Silk Sericin Bead

  • Oh, Hanjin;Lee, Ki Hoon
    • International Journal of Industrial Entomology and Biomaterials
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    • v.27 no.2
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    • pp.329-332
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    • 2013
  • In present preliminary report, we showed the possibility of silk sericin (SS) in enzyme immobilization. SS beads were prepared and enzymes were immobilized on it. The specific activity of immobilized a-chymotrypsin retained more than 87% compared to the free enzyme. The immobilized a-chymotrypsin has better stability against ethanol especially those immobilized on SS beads coagulated in methanol. Immobilized trypsin and lipase had also comparable apparent activity compared to free enzyme. Our result indicates that SS could be a good candidate for enzyme immobilization support due to its hydrophilicity.

Moderating Effects of Temperament on the Association between Maternal Parenting Stress and Behavioral Problems in Preschool Children (어머니의 양육스트레스와 유아의 문제행동의 관계에서 기질의 조절효과)

  • Yi, Yejin;Shin, Yoolim
    • Human Ecology Research
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    • v.57 no.3
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    • pp.369-381
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    • 2019
  • This research investigated the moderating effects of children's negative emotionality, activity and sociability on the relation between maternal parenting stress and children's behavior problems. Participants consisted of 1,667 preschool children from the fifth wave of the Panel Study on Korean Children. EAS was used to measure the children's temperament. Mothers reported parenting stress and children's behavior problems. The results revealed that the magnitude of association between relation between maternal parenting stress and children's behavior problems was greater for high levels of negative emotionality and activity as well as low levels of sociability. The results support a diathesis-stress model in which high negative emotionality, activity and low sociability confer vulnerability for preschool children in a high stress family.

Immobilization of Lysozyme from Hen Egg by Crosslinking Method onto Chitosan Non-woven (키토산 섬유를 담체로 이용한 라이소자임 효소의 고정화)

  • Lee, So Hee
    • Textile Coloration and Finishing
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    • v.30 no.4
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    • pp.264-274
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    • 2018
  • Immobilization of lysozyme on chitosan non-woven using glutaraldehyde(GA) was investigated. For this, 100 % chitosan non-woven was prepared as novel support for the enzyme immobilization. In addition, free lysozyme activity was examined depending on various pH and temperature by measuring time. Moreover, the optimum immobilization conditions depending on various pH, temperature, immobilization time and lysozyme concentration was evaluated. In addition, thermal stability and storage stability of immobilized lysozyme were measured. The characteristics of immobilized lysozyme was examined by FT-IR, surface morphology, and MTT assay. The results are follows: the optimal immobilization of lysozyme were pH 7.0, $25^{\circ}C$, lysozyme concentration 1.5 mg/ml, immobilization time 240 min. The immobilized lysozyme showed higher thermal stability than the free trypsin. The immobilized lysozyme activity was retained 80 % of its initial activity at $4^{\circ}C$ over 30 days of storage. The lysozyme was immobilized effectively on chitosan non-woven by observation of surface morphology.

AMPK activation and adenine nucleotide content in skeletal muscle following different types of high fat feeding

  • Kim, Hyun-Kook;Lee, Songsam
    • Korean Journal of Exercise Nutrition
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    • v.16 no.1
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    • pp.35-41
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    • 2012
  • We investigated the role of fatty acid availability on skeletal muscle AMPK activity and adenine nucleotide content. To investigate the chronic effects of elevated fatty acid in vivo Sprague-Dawley rats were fed a chow diet (15% fat) or a diet high in saturated (SAFA, 52% fat) or polyunsaturated (PUFA, 52% fat) fat for eight weeks. High fat diets increased (P < 0.05) plasma FFA levels by 25%. AMPK activity was increased in SAFA and PUFA rats and occurred in the absence of changes in ATP, AMP, phosphocreatine and glycogen content. These results suggest that increasing fatty acid availability increases AMPK activity independent of changes in the cellular energy charge, and implicate the regulation of AMPK by a covalent mechanism. These data also support the contention that increasing fatty acid availability can increase subsequent fatty acid oxidation by an AMPK-mediated process.

Towards More Accurate Space-Use Prediction: A Conceptual Framework of an Agent-Based Space-Use Prediction Simulation System

  • Cha, Seung Hyun;Kim, Tae Wan
    • International conference on construction engineering and project management
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    • 2015.10a
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    • pp.349-352
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    • 2015
  • Size of building has a direct relationship with building cost, energy use and space maintenance cost. Therefore, minimizing building size during a project development is of paramount importance against such wastes. However, incautious reduction of building size may result in crowded space, and therefore harms the functionality despite the fact that building is supposed to satisfactorily support users' activity. A well-balanced design solution is, therefore, needed at an optimum level that minimizes building size in tandem with providing sufficient space to maintain functionality. For such design, architects and engineers need to be informed accurate and reliable space-use information. We present in this paper a conceptual framework of an agent-based space-use prediction simulation system that provides individual level space-use information over time in a building in consideration of project specific user information and activity schedules, space preference, ad beavioural rules. The information will accordingly assist architects and engineers to optimize space of the building as appropriate.

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A Lifestyle-Routine Activity Theory (LRAT) Approach to Cybercrime Victimization: An Empirical Assessment of SNS Lifestyle Exposure Activities

  • Jihae Suh;Jiseon Choe;Jinsoo Park
    • Asia pacific journal of information systems
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    • v.30 no.1
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    • pp.53-71
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    • 2020
  • The Internet and all of its possibilities and applications have changed individuals' lifestyles in relation to socializing, working, and how they spend their leisure time. Social networking sites (SNSs), such as Facebook or Instagram, are ideal settings for interacting with others but, unfortunately, they are also ideal settings for motivated offenders to commit cybercrimes. Thus SNS users may be more vulnerable to cybercrime. The purpose of this study is to investigate the occurrence of cybercrime victimization, specifically cyber-harassment, cyber-impersonation, and hacking. Self-report surveys collected from a sample of 147 respondents were examined using the moderated multiple regression analysis and a logistic regression analysis to determine possible relationships between SNS lifestyle exposure activities and cybercrime victimization. The results indicate moderate support for the application of lifestyle-routine activity theory (LRAT) to cybercrime victimization. Possible educational and managerial implications, as well as suggestions for future research, are discussed.

Factors Affecting Activity-Based Costing Adoption in Autonomous Public Universities in Vietnam

  • HOANG, Thi Bich Ngoc;PHAM, Duc Hieu;NGUYEN, Thi Minh Giang;NGUYEN, Thi Thanh Phuong
    • The Journal of Asian Finance, Economics and Business
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    • v.7 no.12
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    • pp.877-884
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    • 2020
  • The main aim of the present study is to investigate the factors that are considered affecting the adoption of Activity-Based Costing (ABC) in the autonomous public universities in Vietnam. A quantitative research was carried out. The data was collected through an online questionnaire survey between June 2020 and September 2020 addressed to the target respondents comprising 168 managers and accountants from 23 autonomous public universities in Vietnam, which participated in the resolution project No. 77/NQ-CP of the Vietnamese government on the pilot renovation of operational mechanisms of public tertiary education institutions during the period of four years, from 2014 to 2017. The findings resulted from hypothesis testing by a binary regression analysis based on the SPSS 20.0 software show that training, competition, support of top manager, and university size are the four main factors that positively affect the adoption of Activity-Based Costing (ABC) in studied universities. On the other hand, no statistically significant impact was found on two other variables, namely the variety of services provided and the usefulness of cost information factors. From the research results, discussions and policy recommendations have been presented to promote the adoption of Activity-Based Costing (ABC) in Vietnamese autonomous public universities in the coming time.

Inhibitory effects of KHG26377 on glutamate dehydrogenase activity in cultured islets

  • Yang, Seung-Ju;Hahn, Hoh-Gyu;Choi, Soo-Young;Cho, Sung-Woo
    • BMB Reports
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    • v.43 no.4
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    • pp.245-249
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    • 2010
  • GDH has been known to be related with hyperinsulinism-hyperammonemia syndrome. We have screened new drugs with a view to developing effective drugs modulating GDH activity. In the present work, we investigated the effects of a new drug, KHG26377 on glutamate formation and GDH activity in cultured rat islets. When KHG26377 was added to the culture medium for 24 h prior to kinetic analysis, the $V_{max}$ of GDH was decreased by 59% whereas $K_m$ is not significantly changed. The concentration of glutamate decreased by 50% and perfusion of islets with KHG26377 reduced insulin release by up to 55%. Our results show that KHG26377 regulates insulin release by inhibiting GDH activity in primary cultured islets and support the previous studies for the connection between GDH activity and insulin release. Further studies are required to determine in vivo effects and pharmacokinetics of the drug.

Factors Affecting Physical Activity of Korean Adults in Some County Areas : A Multilevel analysis (군 지역 성인의 신체활동 실천에 미치는 영향요인에 대한 다수준 분석)

  • Kim, Bongjeong
    • Journal of Korean Public Health Nursing
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    • v.30 no.2
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    • pp.311-325
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    • 2016
  • Purpose: This study was conducted to examine the individual and community level factors associated with physical activity and to identify its relative effects using a multilevel analysis among Korean adults in certain counties. Methods: A cross-sectional data of 39,547 adults (age range of 19~64 years) living in 82 counties from the 2013 Korean Community Health Survey (KCHS) was analyzed. Individual and social correlates from KCHS and physical environmental data from the Korean Statistical Information Service were collected. A multilevel logistic regression was performed using Stata 10.0 IC. Results: Multilevel analyses showed that the effect of social and physical environmental on engaging in moderate or vigorous physical activity (MVPA) was significant in comparison to the influence of individual correlates. The individual factors that were associated with participating in MVPA included gender, marital status, education, job, and household income. In the community level, social environmental factors associated with engagement in MVPA were higher satisfaction with healthcare service (OR=3.410, 95% CI=1.109~11.269), a high level of social support (OR=5.920, 95% CI=1.459~22.657) and social network (OR=1.025, 95% CI= 1.017~1.032). Conclusion: To promote moderate or vigorous physical activity in Korean adults in some counties, social environmental factors should be considered along with individual correlates.

Biofilms and their Activity in Granular Activated Carbons Established in a Drinking Water Treatment Plant (정수장 활성탄 여과지의 생물막과 그 활성도)

  • Lee, Ji-Young;Kim, Se-Jun;Chung, Ik-Sang;Joh, Gyeong-Je
    • Journal of Korean Society of Water and Wastewater
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    • v.24 no.6
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    • pp.661-674
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    • 2010
  • Bacterial biomass and its activity were measured in two kinds of granular activated carbon (GAC), the experimental and existing biofiltration system in a drinking water plant. The bacterial biomass was around 210 to 250 nmol P/g WW with phospholipid concentration at acclimation of ozonation treatment. The phospholipid biomass shows more or less a declining gradient along filter depth and no clear seasonality in its values. On the other hand, the microbial activity of [$^3H$]-thymidine and [$^{14}C$]-acetate incorporation within cells increased significantly along the filter depth, showing the difference of three fold between the upper and bottom layer. These factors support the different microbial composition or metabolic activity along the depth of GAC column. Turnover rates, the rate of bacterial biomass and production of biofilm, ranged from 0.26 /hr to 0.37 /hr, indicating a highly rapid recovery itself at amature state. In the non-ozonation treatment, the bacterial biomass was lower than in the ozonation and biological activity also declined towards the filter depth. The biomass levels during cessation of ozonation in the existing GAC filters were 68% of the actively ozonated state.