International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Activity and Stability of Immobilized Enzyme on Silk Sericin Bead

  • Oh, Hanjin (National Instrumentation Center for Environmental Management, Seoul National University) ;
  • Lee, Ki Hoon (Department of Biosystems & Biomaterials Science and Engineering, Seoul National University)
  • Received : 2013.12.10
  • Accepted : 2013.12.17
  • Published : 2013.12.31
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Abstract

In present preliminary report, we showed the possibility of silk sericin (SS) in enzyme immobilization. SS beads were prepared and enzymes were immobilized on it. The specific activity of immobilized a-chymotrypsin retained more than 87% compared to the free enzyme. The immobilized a-chymotrypsin has better stability against ethanol especially those immobilized on SS beads coagulated in methanol. Immobilized trypsin and lipase had also comparable apparent activity compared to free enzyme. Our result indicates that SS could be a good candidate for enzyme immobilization support due to its hydrophilicity.

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References

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Cited by

  1. Preparation and Characterization of Silk Beads for Protein Delivery System vol.28, pp.2, 2014, https://doi.org/10.7852/ijie.2014.28.2.66
  2. Catalytic Activity of Immobilized Chymotrypsin on Hybrid Silica-Magnetic Biocompatible Particles and Its Application in Peptide Synthesis vol.190, pp.4, 2020, https://doi.org/10.1007/s12010-019-03158-z