Acknowledgement
This work was supported by the National Research Foundation of Korea Grant funded by the Korean Government [NRF-2009-351-G00151]
DOI QR Code
AMPK activation and adenine nucleotide content in skeletal muscle following different types of high fat feeding
- Kim, Hyun-Kook (Department of Physical Education, Daegu University) ;
- Lee, Songsam (Department of Physical Education, Daegu University)
- Received : 2012.02.17
- Published : 2012.03.31
Abstract
We investigated the role of fatty acid availability on skeletal muscle AMPK activity and adenine nucleotide content. To investigate the chronic effects of elevated fatty acid in vivo Sprague-Dawley rats were fed a chow diet (15% fat) or a diet high in saturated (SAFA, 52% fat) or polyunsaturated (PUFA, 52% fat) fat for eight weeks. High fat diets increased (P < 0.05) plasma FFA levels by 25%. AMPK activity was increased in SAFA and PUFA rats and occurred in the absence of changes in ATP, AMP, phosphocreatine and glycogen content. These results suggest that increasing fatty acid availability increases AMPK activity independent of changes in the cellular energy charge, and implicate the regulation of AMPK by a covalent mechanism. These data also support the contention that increasing fatty acid availability can increase subsequent fatty acid oxidation by an AMPK-mediated process.