• Reproductive and Developmental Biology
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• v.32 no.1
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• pp.15-20
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• 2008

Pronuclear DNA microinjection has been the most universal method in transgenic animal production but its success rate of transgenesis in mammals are extremely low. To address this long-standing problem, we used retrovirus- and lentivirus-based vectors carrying the enhanced green fluorescent protein (EGFP) gene under the control of ubiquitously active cytomegalovirus (CMV) promoter to deliver transgenes to bovine embryos. The rate of transgenesis was evaluated by counting EGFP positive blastocysts after injection of concentrated virus stock into the perivitelline space of the bovine oocytes in metaphase II. Among two different types of lentivirus vectors derived from FIV (feline immunodeficiency virus) and HIV (human immunodeficiency virus), the former scored the higher gene transfer efficiency; almost 100% of the blastocysts developed from the oocytes infected with FIV-based vector were EGFP positive. As for the vectors derived Com HIV lentivirus, the transgenesis rate of the blastocysts was reduced to 39%.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.162-165
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• 2003

In order to study the secretion of the human urokinase-type plasminogen activator, u-PA, from the yeast yarrowia lipolytica, three kinds of integrative expression vector were constructed. These vectors differed only in their secretion control legions, pre-, pre-dip-(dipeptide Stretch) or pre-dip-pro sequences of the alkaline extracellular protease, which were joined inflame to the human u-PA cDNA. The recombinant Y. lipolytica Strains, transformed with the expression vectors, secreted the hyperglycosylated u-PA. A fibrin plate assay of the culture supernatants showed that the hyperglycosylated u-PA proteins could catalyze fibrinolysis, and that the pre-dip sequence was the most efficient secretory signal for the secretion of the u-PA from Y. lipolyica. This result suggests that Y. lipolytica can be developed as a potential host for the production of recombinant human u-PA.

• Microbiology and Biotechnology Letters
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• v.27 no.5
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• pp.364-369
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• 1999

In order to obtain the suitable plasmids for constructing plasmid vectors of Bacillus species, endogeneous plasmid DNAs were screended from thermo-tolerant soil bacteria. Based on agarose gel electrophoresis patterns of the isolated plasmid DNAs, two strains harboring small-size plasmids were selected. The isolated were identified to belong to the genus Bacillus on the basis of their morphological and biochemical properties, and named Bacillus sp. 3-3 and 77-8, respectively. The restriction endonuclease maps were determined for four plasmids including two plasmids from each Bacillus isolates. It is interesting that Bacillus sp. 3-3 and 77-8 have an identical plasmid according to the restriction maps. The three kinds of hybrid plasmids constructed by introducing each plasmid of two isolates into a Escherichia coli plasmid vector. pUCCm18 containing chloramplenicol resistance gene active in Bacillus strains, could be replicated in B. subtilis and B. licheniformis. These plasmids are very stable in B. subtilis, suggesting that the Bacillus plasmids identified in this work would be useful for development of new cloning vectors for Bacillus strains.

• YAKHAK HOEJI
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• v.47 no.3
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• pp.176-179
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• 2003

A library of unlimited number of novel lectins with diverse specificities has been previously generated by randomly mutating the carbohydrate-recognition domain of Maackia amurensis hemagglutinin (MAH). To establish the experimental environment capable of selecting high affinity mutant lectins in E. coli, phage display system was adapted. Carbohydrate binding capacity of two phagemid vectors, pComb3 and pComb8 displaying wild-type MAH lectin was assessed. Specific bindings of pComb3 and pComb8 phages expressing w.t. MAH to affinity-purified polyclonal anti-MAH antibody and to glycophorin was demonstrated. Both phages also showed strong hemagglutinating activity to intact but not sialidase-treated human erythrocytes, which is consistent to the specificity of native MAH. Taken together, two different phage display vectors successfully allowed the expression of active MAH as a fusion protein on the surface of bacteriophage, which will lead to preparation of unique plant lectins with high affinity toward a variety of carbohydrate chains.

• Journal of Power Electronics
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• v.15 no.3
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• pp.796-805
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• 2015

New direct and indirect current control methods for a fault-tolerant active power filter topology are presented in this paper. Since a three-phase four-switch topology has a phase bridge current which cannot be directly controlled, a hysteresis control method in the α-β plane which controls the three-phase current in the two-phase stationary coordinate system is proposed. The improved SVPWM algorithm is able to eliminate the operation of the trigonometric functions in the traditional algorithm by rotating the α-β coordinates and alternating the sequence of the output vectors, which in turn simplifies the algorithm and reduces the switching frequency. The selection of the DC-side reference voltage and DC-side capacitor equalization strategy are also discussed. Simulation and experiments demonstrate that the proposed control method is correct and feasible.

• Journal of Power Electronics
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• v.15 no.1
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• pp.106-115
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• 2015

This paper proposes a novel direct power control (DPC) strategy for neutral-point-clamped (NPC) three-level rectifiers, to directly control the active power, the reactive power and the neutral point potential of the rectifiers by referring to three pre-calculated vector influence tables and minimizing an objective function. In the three vector influence tables, the influences of different voltage vectors on the active power, the reactive power and the neutral-point potential are shown explicitly. A conceptual description and control algorithm of the proposed controller are presented in this paper. Then, numerical simulations and experiments are carried out to validate the proposed method. Both the simulation and experimental results show that good performances during both the steady-state and transient operating conditions are achieved. As a result, the proposed strategy has been proven to be effective for NPC three-level rectifiers.

• Journal of the Korean Institute of Intelligent Systems
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• v.21 no.4
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• pp.512-517
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• 2011

In this paper, we propose an Active Shape Model (ASM) and Lucas-Kanade (LK) optical flow-based feature extraction and analysis method for analyzing the emotional features from facial images. Considering the facial emotion feature regions are described by Facial Action Coding System, we construct the feature-related shape models based on the combination of landmarks and extract the LK optical flow vectors at each landmarks based on the centre pixels of motion vector window. The facial emotion features are modelled by the combination of the optical flow vectors and the emotional states of facial image can be estimated by the probabilistic estimation technique, such as Bayesian classifier. Also, we extract the optimal emotional features that are considered the high correlation between feature points and emotional states by using common spatial pattern (CSP) analysis in order to improvise the operational efficiency and accuracy of emotional feature extraction process.

• Steel and Composite Structures
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• v.32 no.5
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• pp.671-686
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• 2019

Active control of solar panels with honeycomb core and carbon nanotube reinforced composite (CNTRC) facesheets for smart structures using piezoelectric patch sensor and actuator to reduce the amplitude of vibration is a lack of the previous study and it is the novelty of this research. Of active control elements are piezoelectric patches which act as sensors and actuators in many systems. Their low power consumption is worth mentioning. Thus, deriving a simple and efficient model of piezoelectric patch's elastic, electrical, and elastoelectric properties would be of much significance. In the present study, first, to reduce vibrations in composite plates reinforced by carbon nanotubes, motion equations were obtained by the extended rule of mixture. Second, to simulate the equations of the system, up to 36 mode shape vectors were considered so that the stress strain behavior of the panel and extent of displacement are thoroughly evaluated. Then, to have a more acceptable analysis, the effects of external disturbances (Aerodynamic forces) and lumped mass are investigated on the stability of the system. Finally, elastoelectric effects are examined in piezoelectric patches. The results of the present research can be used for micro-vibration suppression in satellites such as solar panels, space telescopes, and interferometers and also to optimize active control panel for various applications.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.639-647
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• 2008

The heat-inducible expression vectors for Corynebacterium glutamicum and C. ammoniagenes were constructed by using the ${\lambda}O_L1$ and the cryptic promoters, CJ1 and CJ4 that express genes constitutively in C. ammoniagenes. Although the promoters were isolated from C. ammoniagenes, CJ1 and CJ4 were also active in C. glutamicum. To construct vectors, the $O_L1$ from the ${\lambda}P_L$ promoter was isolated and fused to the CJ1 and CJ4 promoters by recombinant PCR. The resulting artificial promoters, CJ1O and CJ4O, which have one ${\lambda}O_L1$, and CJ1OX2, which has two successive ${\lambda}O_L1$, were fused to the green fluorescent protein (GFP) gene followed by subcloning into pCES208. The expression of GFP in the corynebacteria harboring the vectors was regulated successfully by the temperature-sensitive cI857 repressor. Among them, C. ammoniagenes harboring plasmid pCJ1OX2G containing GFP fused to CJ1OX2 showed more GFP than the other ones and the expression was tightly regulated by the repressor. To construct the generally applicable expression vector using the plasmid pCJ1OX2G, the His-tag, enterokinase (EK) moiety, and the MCS were inserted in front of the GFP gene. Using the vector, the expression of pyrR from C. glutamicum was tried by temperature shift-up. The results indicated that the constructed vectors (pCeHEMG) can be successfully used in the expression and regulation of foreign genes in corynebacteria.

• 제어로봇시스템학회:학술대회논문집
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• 2004.08a
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• pp.312-316
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• 2004

The paper presents a technique to identify the boundary of the optic disc in infant retinal digital images using an approach based on active contours (snakes). The technique can be used to be develop a automate system in order to help the ophthalmologist's diagnosis the retinopathy of prematurity (ROP) disease which may occurred on preterm infant,. The optic disc detection is one of the fundamental step which could help to create an automate diagnose system for the doctors we use a new kind of active contour (snake) method has been developed by Chenyang et. al. [1], based on a new type of external force field, called gradient vector flow, or GVF. GVF is computed as a diffusion of the gradient vectors of a gray-level or binary edge map derived from the image. The testing results on a set of infant retinal ROP images verify the effectiveness of the proposed methods. We show that GVF has a large capture range and it's able to move snakes into boundary concavities of optic disc and finally the optic disk boundary was determined.