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Functional Display of Maackia amurensis Hemagglutinin (MAH) on Bacteriophage  

임미정 (숙명여자대학교 약학대학)
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YAKHAK HOEJI / v.47, no.3, 2003 , pp. 176-179 More about this Journal
Abstract
A library of unlimited number of novel lectins with diverse specificities has been previously generated by randomly mutating the carbohydrate-recognition domain of Maackia amurensis hemagglutinin (MAH). To establish the experimental environment capable of selecting high affinity mutant lectins in E. coli, phage display system was adapted. Carbohydrate binding capacity of two phagemid vectors, pComb3 and pComb8 displaying wild-type MAH lectin was assessed. Specific bindings of pComb3 and pComb8 phages expressing w.t. MAH to affinity-purified polyclonal anti-MAH antibody and to glycophorin was demonstrated. Both phages also showed strong hemagglutinating activity to intact but not sialidase-treated human erythrocytes, which is consistent to the specificity of native MAH. Taken together, two different phage display vectors successfully allowed the expression of active MAH as a fusion protein on the surface of bacteriophage, which will lead to preparation of unique plant lectins with high affinity toward a variety of carbohydrate chains.
Keywords
Lectin; Maackia amurensis hemagglutinin (MAH); phage display system;
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  • Reference
1 Lambda foo: a lambda phage vector for the expression of foreign proteins /
[ Maruyama, I. N.;Maruyama, H. I.;Brenner, S. ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
2 Application of a filamentous phage pⅧ fusion protein system suitable for efficient produciton, screening, and mutagenesis of F(ab) antibody fragments /
[ Huse, W. D.;Stinchcombe, T. J.;Glaser, S. M.;Starr, L.;MacLean, M.;Hellstrom, K. E.;Hellstrom, I.;Yelton, D. E. ] / J. Immunol.
3 An unusual carbohydrate binding site revealed by the structures of two Maackia amurensis lectins complexed with sialic acid-containing oligosaccharides /
[ Imberty, A.;Gautier, C.;Lescar, C.;Lescar, J.;Perez, S.;Wyns, L.;Loris, R. ] / J. Biol. Chem.   DOI   ScienceOn
4 Sialic acid-binding motif of Maackia amurensis lectins /
[ Yamamoto, K.;Konami, Y.;Irimura, T. ] / J. Biochem. (Tokyo)   DOI   ScienceOn
5 Diversity in the sialic acids /
[ Varki, A. ] / Glycobiology   DOI   ScienceOn
6 Variations in activity of the human natural anti-Gal antibody in young and elderly populations /
[ Wang, L.;Anaraki, F.;Henion, T. R.;Galili, U. ] / J. Gerontol. A. Biol. Sci. Med. Sci.
7 Elucidation of lectin receptors by quantitative inhibition of lectin binding to human erythrocytes and lymphocytes /
[ Kawaguchi, T.;Osawa, T. ] / Biochemistry   DOI   ScienceOn
8 Glycosylation defining cancer malignancy : new wine in an old bottle /
[ Hakomori, S. ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
9 Assembly of combinatorial antibody libraries on phage surfaces: the gene III site /
[ Barbas, C. F. 3rd;Kang, A. S.;Lerner, R. A.;Benkovic, S. J. ] / Proc. Natl. Acad. Sci. U.S.A.   DOI
10 Introduction to antibody engineering and phage display /
[ Watkins, N. A.;Ouwehand, W. H. ] / Vox Sang   DOI   ScienceOn
11 of Maackia amurensis hemagglutinin (MAH) for sialic acid-containing Ser/Thr-linked carbohydrate chains of N-terminal octapeptides from human glycophorin A /
[ Konami, Y.;Yamamoto, K.;Osawa, T.;Irimura, T. ] / FEBS. Lett.   DOI   ScienceOn
12 Cloning of anti-Gal Fabs from combinatorial phage display libraries: structural analysis and comparison of Fab expression in pComb3H and pComb8 phage /
[ Wang, L.;Radic, M. Z.;Siegel, D.;Chang, T.;Bracy, J.;Galili, U. ] / Mol. Immunol.   DOI   ScienceOn
13 Mutated plant lectin library useful to identify different cells /
[ Yim, M.;Ono, T.;Irimura, T. ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
14 Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries /
[ Kang, A. S.;Jones, T. M.;Burton, D. R. ] / Proc. Natl. Acad. Sci. U.S.A.   DOI   ScienceOn
15 A journey to the world of glycobiology /
[ Kobata, A. ] / Glycoconj. J.   DOI   ScienceOn
16 Efficient epitope mapping by bacteriophage lambda surface display /
[ Kuwabara, I.;Maruyama, H.;Mikawa, Y. G.;Zuberi, R. I.;Liu, F. T.;Maruyama, I. N. ] / Nat. Biotechnol.   DOI   ScienceOn
17 A unique amino acid sequence involved in the putative carbohydrate-binding domain of a legume lectin specific for sialylated carbohydrate chains: primary sequence determination of Maackia amurensis hemagglutinin (MAH) /
[ Konami, Y.;Ishida, C.;Yamamoto, K.;Osawa, T.;Irimura, T. ] / J. Biochem.(Tokyo)   DOI
18 Polysialylation: from bacteria to brains /
[ Troy, F. A. 2nd. ] / Glycobiology   DOI   ScienceOn