• 복식문화연구
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• 제3권2호
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• pp.323-346
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• 1995

The increasing number of senior citizens, combined with the power of purchasing due to discretionary income have vaulted the elderly into the position of an attractive future target market. Therefore, it would be crucial for marketers to understand elderly's purchasing behavior. The purpose of this study was to identify clothing purchasing behavior of elderly women. For this study, questionnaires were administered to 600 women over 55 years of age. However, the sample that was analyzed fer statistical analysis was involved 418 elderly women. Statistical analysis were majorly descriptives such as frequencies and percentages. The major results of this study were summarized as follows. 1. In relation to problem recognition in purchasing process, motives of purchasing apparel were identified as a happy event in home such as a wedding and a birthday, a change of seasons and a casual discovery of a suitable clothing in shopping, in orders. 2. In relation to information search, important information on apparel and fashionability were thought as display racks in a store, opinion from friends and family and fashionability from others or streets, in orders. 3. In relation to selecting a store in purchasing process, older consumers assessed that attractive price, design suited to my age, variety in one store and apparel product quality were important store attributes, in orders. In terms of a purchase place, older consumers purchased clothing mainly on department stores, wholesale stores such as Namdaemoon or Dongdaemoon market, mainly retail stores located close to home and discount stores of well known brand, in orders. 4. In relation to alternative evaluation in purchasing process, older consumeres considered that style or appearance suited to me, color, design, comfort and fitness were important selection criteria, in orders. 5. In relation to purchase choice, 61.7% of the respondents paid money by themselves and 68.9% paid on cash in purchasing apparel. 6. In relation to outcomes of purchase, older consumers solved their complaint against a unsatisfactory product mainly by returning the unsatisfactory clothing. Also, there were those who took no action against the unsatisfactory product and who altered the clothing for fitness by themselves.

• BMB Reports
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• 제37권3호
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• pp.292-297
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• 2004

The current model for the mechanism of action of the Bacillus thuringiensis Cry $\delta$-endotoxins involves the penetration of the ${\alpha}4-{\alpha}5$ hairpin into the target midgut epithelial cell membranes, followed by pore formation. In this study, PCR-based mutagenesis was employed to identify a critical residue within the ${\alpha}4-{\alpha}5$ loop of the 130-kDa Cry4A mosquito-larvicidal protein. Alanine-substitutions of two charged (Asp-198 and Asp-200) and four polar (Asn-190, Asn-195, Tyr-201 and Tyr-202) residues in the ${\alpha}4-{\alpha}5$ loop were performed. Like the wild-type, all of the mutant toxins were over-expressed as inclusion bodies in Escherichia coli. When E. coli cells expressing each mutant toxin were bioassayed against Aedes aegypti larvae, larvicidal activity was completely abolished for the substitution of only Tyr-202, while replacements at the other positions still retained a high level of toxicity. Further replacement of Tyr-202 with an aromatic side chain, phenylalanine, did not affect the toxicity. These results revealed a crucial role in toxin activity for the conserved aromatic residue at the 202 position within the ${\alpha}4-{\alpha}5$ loop of the Cry4A toxin.

• 정보학연구
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• 제6권3호
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• pp.151-168
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• 2003

본 연구는 기본적으로 온라인과 오프라인의 통합마케팅을 설명하고 이에 관한 경험적 사례를 개발하기 위한 목적으로 진행되었다. 특히 중소 유통영역의 공생적 기반 위에 전개되는 온-오프라인의 경험적 사례를 개발하고 바람직한 마케팅방향을 방향을 제시하였다. 본 연구는 문헌연구와 사례연구를 병행하여 연구를 진행하였다. 사례는 인터넷 도매유통의 공생협력을 추진한 (주)Barokosa이다. 온-오프라인의 협력적 통합마케팅의 전략을 전개하기 위해서는 무엇보다도 온-오프라인의 뚜렷한 목표를 설정하고 성장방향에 대한 비전을 공유하고 나아가 온-오프라인의 사명을 감당하는 것이다. 실천적으로는 (1)항상 고객(customer)기반의 의사결정을 이루며 (2)철저한 협력적 돕는경쟁(competition) 의식과 (3)구성원 자신의 일에 대한 자신감(confidence)을 지니고 (4)실천을 위한 용기(courage)를 가지고 (5)혁신하여 변화(change)를 선도하는 것이다.

• 해양환경안전학회지
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• 제24권1호
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• pp.10-17
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• 2018

종합 안전관리체계인 e-navigation을 준비하기 위해 선박의 의도된 경로를 선박 대 선박, 그리고 선박 대 육상 간에 공유하는 경로교환에 대한 연구가 활발히 진행 중이다. 하지만 경로교환을 위한 기술적인 측면에서의 연구 개발은 진행되고 있으나, 그 효과나 문제점 등 도입 필요성에 대한 연구는 부족한 실정이다. 본 연구는 선박조종 시뮬레이션을 통해 경로교환이 항해 안전에 미치는 영향을 정량적으로 분석하고, 시뮬레이션 수행자들의 주관적인 평가를 종합하여 경로교환의 효과를 검증하였다. 선박 간 경로교환을 할 경우 최초 충돌회피 시점은 평균 3.43분 더 빨라졌으며, 충돌회피 방향은 60 %가 변경되었다. 또한 타선 A와의 최근접점은 31 % 더 가까워지고, 평균 타 사용지수는 57 % 줄어들었다. 그리고 설문 응답자의 95 %는 경로교환이 충돌회피 의사결정에 영향, 85 %는 안전운항에 긍정적인 영향, 90 %는 사고예방 효과, 70 %는 항해사의 심리적 부담 경감, 70 %는 경로교환을 실무에 도입해야한다고 답하였다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권4호
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• pp.325-336
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• 2000

Bone morphogenetic protein-2/4 are members of Transforming Growth Factor-$\beta$(TGF-$\beta$) superfamily and they may induce formation of cartilage and bone in vivo. This study was performed to investigate the cellular target and period of action of BMP-2/4 and understanding of actions of BMP-2/4 at cellular level. The appearance of BMP-2/4 during healing of mandibular and periodontal defect in rat was evaluated immunohistochemically. 40 Sprague-Dawley strain white male rats, each weighing about 300gm were used. Bony defect was performed in the mandible and they were sacrificed at the day of 3rd, 10th, 20th, 30th after operation. The specimens were harvested and examined histologically and immunohistochemically by localization of anti-BMP-2/4. The results were as follows: 1. Woven bone was observed at 10th day and perfect healing of defect with compact bone and periodontal ligment space at 30th day. 2. Osteoprogenitor cells, osteoblastic cells and periosteum were positive reaction to immunohistochemical stain at 10th day. 3. Cells of bone marrow space and surface cells of osteocytes and cementoblasts were positive reaction to immunohistochemical stain at 20th day. 4. Newly formed osteocytes and cementocytes were positive reaction to immunohistochemical stain at 30th day. From the above findings, we could conclude that BMP-2/4 acted significant roles as factors of induction, proliferation and differentiation during bone healing process.

• 한국건설순환자원학회논문집
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• 제2권1호
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• pp.46-51
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• 2014

본 연구에서는 단위수량 저감에 따른 하이볼륨 플라이애시 콘크리트의 강도 발현 특성을 평가하였으며, 목표강도 3수준 (40, 30 및 20MPa) 및 단위수량 변화에 따라 콘크리트를 제조하였다. 실험결과, 향상된 유동성은 50%이상의 플라이애시를 사용한 경우 플라이애시 구형 입자들의 볼베어링 효과, 플라이애시의 정전기적 반발력 및 플라이애시의 입도분포의 결과로 얻어졌으며, 이를 통하여 플라이애시 혼합이 콘크리트에 요구되는 물의 양을 감소시키는 것으로 나타났다. 또한 단위수량을 저감한 하이볼륨 플라이애시 콘크리트의 조기 강도는 일반적인 단위수량의 배합보다 약 66% 이상 향상되는 것으로 나타났다.

• Journal of Ginseng Research
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• 제39권2호
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• pp.125-134
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• 2015

Background: Black ginseng (Ginseng Radix nigra, BG) refers to the ginseng steamed for nine times and fine roots (hairy roots) of that is called fine black ginseng (FBG). It is known that the content of saponin of FBG is higher than that of BG. Therefore, in this study, we examined antitumor effects against MCF-7 breast cancer cells to target the FBG extract and its main component, ginsenoside Rg5 (Rg5). Methods: Action mechanism was determined by MTT assay, cell cycle assay and western blot analysis. Results: The results from MTT assay showed that MCF-7 cell proliferation was inhibited by Rg5 treatment for 24, 48 and 72 h in a dose-dependent manner. Rg5 at different concentrations (0, 25, 50 and $100{\mu}M$), induced cell cycle arrest in G0/G1 phase through regulation of cell cycle-related proteins in MCF-7 cells. As shown in the results from western blot analysis, Rg5 increased expression of p53, $p21^{WAF1/CIP1}$ and $p15^{INK4B}$ and decreased expression of Cyclin D1, Cyclin E2 and CDK4. Expression of apoptosiserelated proteins including Bax, PARP and Cytochrome c was also regulated by Rg5. These results indicate that Rg5 stimulated cell apoptosis and cell cycle arrest at G0/G1 phase via regulation of cell cycle-associated proteins in MCF-7 cells. Conclusion: Rg5 promotes breast cancer cell apoptosis in a multi-path manner with higher potency compared to 20(S)-ginsenoside Rg3 (Rg3) in MCF-7 (HER2/ER+) and MDA-MB-453 (HER2+/ER) human breast cancer cell lines, and this suggests that Rg5 might be an effective natural new material in improving breast cancer.

• 한국동물생명공학회지
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• 제37권3호
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• pp.155-161
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• 2022

Pregnancy-associated plasma protein-A (PAPP-A) is known as an important biomarker for fetal abnormality during first trimester and has a pivotal role in follicle development and corpus luteum formation. And also, it is being revealed that an expression of PAPP-A in various cells and tissues such as cancer and lesion area. PAPP-A is the major IGF binding protein-4 (IGFBP-4) protease. Cleavage of IGFBP-4 results in loss of binding affinity for IGF, causing increased IGF bioavailability for proliferation, survival, and migration. Additionally, PAPP-A can be used as a promising therapeutic target for healthy longevity. Despite growing interest, almost nothing is known about how PAPP-A expression is regulated in any tissue. This review will focus on what is currently known about the zinc metalloproteinase, PAPP-A, and its role in cells and tissues. PAPP-A is expressed in proliferating cells such as fetus in uterus, granulosa cells in follicle, dermis in wound, cancer cells, and Sertoli cells in testis. They have common characteristics of proliferation faster than normal cells with stimulating IGFs action and inhibiting IGFBPs. The PAPP-A functions and expression studies in livestock have not yet been conducted much. Further studies are needed to use PAPP-A as a marker for healthy longevity in animal science.

• Animal Bioscience
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• 제36권2호
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• pp.175-190
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• 2023

Objective: The study was conducted to screen differentially expressed long noncoding RNA (lncRNA) in chickens by high-throughput sequencing and explore its mechanism of action on intramuscular fat deposition. Methods: Herein, Rose crown and Cbb broiler chicken embryo breast and leg muscle lncRNA and mRNA expression profiles were constructed by RNA sequencing. A total of 96 and 42 differentially expressed lncRNAs were obtained in Rose crown vs Cobb broiler chicken breast and leg muscle, respectively. lncRNA-ENSGALT00000046546, with high interspecific variability and a potential regulatory role in lipid metabolism, and its predicted downstream target gene 1-acylglycerol-3-phosphate-O-acyltransferase 2 (AGPAT2), were selected for further study on the preadipocytes. Results: lncRNA-46546 overexpression in chicken preadipocyte 2 cells significantly increased (p<0.01) the expression levels of AGPAT2 and its downstream genes diacylglycerol acyltransferase 1 and diacylglycerol acyltransferase 2 and those of the fat metabolism-related genes peroxisome proliferator-activated receptor γ, CCAAT/enhancer binding protein α, fatty acid synthase, sterol regulatory element-binding transcription factor 1, and fatty acid binding protein 4. The lipid droplet concentration was higher in the overexpression group than in the control cells, and the triglyceride content in cells and medium was also significantly increased (p<0.01). Conclusion: This study preliminarily concludes that lncRNA-46546 may promote intramuscular fat deposition in chickens, laying a foundation for the study of lncRNAs in chicken early embryonic development and fat deposition.

• 인간행동과 음악연구
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• 제14권2호
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• pp.19-43
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• 2017

본 연구는 만 4-5세의 자폐범주성장애 아동 4명을 대상으로 음악 조건과 비음악 조건의 단계적 상호 주의 중재를 교대로 실시하여 공동주의 기술 향상에 미치는 중재 효과를 비교하고자 하였다. 이를 위해 단일대상연구의 한 가지 방법인 교대중재설계를 사용하였고, 음악 조건과 비음악 조건의 중재를 사전에 결정된 무작위 순서에 따라 회기 내 교대로 실시하였다. 총 23회기의 전체 프로그램은 기초선(3회기), 중재(15회기), 유지(5회기)의 절차로 이루어졌다. 음악 조건에서는 구조화된 공동 악기 연주, 음악적 신호(cue)에 따른 주의전환, 상호적 악기연주가 이루어졌고, 비음악 조건에서는 조립장난감을 이용한 공동 놀이, 언어적 신호에 따른 주의전환, 장난감 주고받기가 이루어졌다. 중재 내 나타난 공동주의 행동 변화는 공동주의 반응행동과 얼굴 쳐다보기, 공동 활동에 참여하기의 발생 빈도로 측정되었고, 중재 사전 사후에는 초기 사회-의사소통 평가를 실시하였다. 연구 결과, 두가지 조건 모두에서 공동주의 행동이 증가하는 추이를 보였으나, 음악 조건이 비음악 조건에 비해 증가하는 폭이 크고 더 높은 수준에서 유지되는 것을 확인할 수 있었다. 본 연구에서 도출된 결과는 구조화된 음악적 환경 안에서, 음악을 통해 사회적 정보를 처리하고 주의를 조절하는 중재가 자폐범주성장애 아동의 공동주의 행동을 향상시키는 데 효과적임을 시사한다.